Jayne Martin Carli, PhD, CLC

Education

• Doctor of Philosophy (Mar 2012 - Aug 2017) Department of Biochemistry & Molecular Biophysics; Naomi Berrie Diabetes Center Laboratory of Rudolph Leibel, MD, Columbia University Medical Center, New York, NY
  • "RPGRIP1L and FTO - genes implicated in the effects of FTO intronic sequence variants on food intake - also affect adipogenesis and adipocyte biology."
• Master of Philosophy (Aug 2009 - Feb 2012) Department of Biochemistry & Molecular Biophysics; Naomi Berrie Diabetes Center Laboratory of Rudolph Leibel, MD, Columbia University Medical Center, New York, NY
• Master of Science (Sep 2005 - May 2007) Department of Biology, Laboratory of Laura Schramm, PhD
• "TFIIIB-regulated transcription by RNA polymerase III in cancer cells"
• Bachelor of Science (Sep 2002 - May 2006) Magna cum Laude, Department of Biology, Laboratory of Laura Schramm, PhD, St. John's University, Queens, NY
  • "TFIIIB-regulated transcription by RNA polymerase III in cancer cells"

Contact
Research Complex 2
12700 East 19th Avenue
Room 3101, MS 8613
Aurora, CO 80045

Phone: (303) 724-3504
Fax: (303) 724-3512

Research Interests
My work focuses on the molecular biology of lactation and its relationship to metabolic disease. I conducted my graduate work at Columbia University Medical Center under the guidance of Rudolph Leibel, MD. There, I investigated genetic determinants of adipocyte development, which impact body weight regulation and metabolic health. After the birth of my daughter in 2016, I became acutely aware of the links between maternal metabolic health and lactation success. I sought a postdoctoral fellowship at CU-AMC to address important knowledge gaps regarding the mechanisms controlling human lactation, which have important implications for the long-term metabolic health of neonates and their mothers. I received mentorship and training in the physiology and molecular biology of lactation from Drs. James McManaman & Jenifer Monks and clinical research training and mentorship on diabetes in pregnancy from Drs. Linda Barbour, Teri Hernandez and Jacob Friedman. With support from the Colorado Nutrition Obesity Research Center, I worked to identify how to best utilize human milk samples as “liquid breast biopsies” to interrogate human mammary function non-invasively. I published the first profile of cells from human milk by single cell RNA sequencing and I conducted comparative proteomics to illuminate the mechanisms of milk fat globule secretion. I am currently using human milk samples to identify the mechanisms underlying poor milk production in participants with obesity and/or gestational diabetes, with support from the National Institute of Child Health and Human Development and the Ludeman Family Center for Women's Health Research. My future work will continue to expand our experimental capabilities in human lactation and will focus on the mechanisms underlying mammary function in humans. In particular, I am interested in the ability of the mammary epithelium to coordinate with other maternal organs to drive nutrient uptake and transformation into complete infant nutrition.

Full List of Publications

Recent Publications

1. Martin Carli JF, Dzieciatkowska M, Hernandez TL, Monks J, McManaman JL. Comparative proteomic analysis of human milk fat globules and paired membranes and mouse milk fat globules identifies core cellular systems contributing to mammary lipid trafficking and secretion. Front Mol Biosci. 2023;10:1259047. Published 2023 Dec 18. doi:10.3389/fmolb.2023.1259047
2. Neville MC, Demerath EW, Hahn-Holbrook J, et al. Parental factors that impact the ecology of human mammary development, milk secretion, and milk composition-a report from "Breastmilk Ecology: Genesis of Infant Nutrition (BEGIN)" Working Group 1. Am J Clin Nutr. 2023;117 Suppl 1(Suppl 1):S11-S27. doi:10.1016/j.ajcnut.2022.11.026
3. Martin Carli JF, Trahan GD, Rudolph MC. Resolving Human Lactation Heterogeneity Using Single Milk-Derived Cells, a Resource at the Ready. J Mammary Gland Biol Neoplasia. 2021;26(1):3-8. doi:10.1007/s10911-021-09489-0
4. Martin Carli JF, Trahan GD, Jones KL, et al. Single Cell RNA Sequencing of Human Milk-Derived Cells Reveals Sub-Populations of Mammary Epithelial Cells with Molecular Signatures of Progenitor and Mature States: a Novel, Non-invasive Framework for Investigating Human Lactation Physiology. J Mammary Gland Biol Neoplasia. 2020;25(4):367-387. doi:10.1007/s10911-020-09466-z
5. Martin Carli JF, LeDuc CA, Zhang Y, Stratigopoulos G, Leibel RL. FTO mediates cell-autonomous effects on adipogenesis and adipocyte lipid content by regulating gene expression via 6mA DNA modifications. J Lipid Res. 2018;59(8):1446-1460. doi:10.1194/jlr.M085555
6. Carli JFM, LeDuc CA, Zhang Y, Stratigopoulos G, Leibel RL. The role of Rpgrip1l, a component of the primary cilium, in adipocyte development and function. FASEB J. 2018;32(7):3946-3956. doi:10.1096/fj.201701216R
7. Kilpeläinen TO, Carli JF, Skowronski AA, et al. Genome-wide meta-analysis uncovers novel loci influencing circulating leptin levels. Nat Commun. 2016;7:10494. Published 2016 Feb 1. doi:10.1038/ncomms10494