By Lisa Marshall
Research by CU scientists published last summer uses
The findings, published in the journal Genes and Development, also shed new light on just how epigenetic changes—the switching on or off of genes—happen inside the cell.
“Many cancers make use of epigenetic gene silencing to promote their own growth. Medical scientists want to inhibit this cancer-causing process, but they first need to know exactly how it works,” said Thomas Cech,
For the study, Cech and lead author Daniel Youmans, who is pursuing a
Then, they used a high-tech microscope to observe what happens to the proteins inside living human cells, both under normal conditions and when exposed to a cancer drug called A-395, which is a PRC2 inhibitor.
“Our work helps define the mechanism of these cancer drugs and changes what we thought about the way PRC2 functions inside cells,” Youmans said.
PRC2 is made of a cluster of four core proteins, which interact with other proteins circulating in the cell. When they click together just right, like puzzle pieces, it signals the complex to make its way to certain genes in the cell, silencing them, said Youmans.
In a healthy cell, that silencing can influence whether a stem cell becomes a neuron or heart cell and shut off genes which could promote disease. But in many cancers, including lymphoma, the complex is hijacked to silence tumor suppressors.
Scientists previously thought that PRC2 cancer therapeutics worked by preventing the complex from binding to genes. But when watching it swirl around the cell, the researchers discovered that in the presence of the drug, the molecular silencing machine made it to its locations. Once there, it just failed to fully do its job.
They also identified specific auxiliary proteins which PRC2 must click to in order to be recruited to its target genes.
“We showed that when you disrupt the interaction between PRC2 and these other puzzle pieces, it completely disrupts its ability to bind to specific places,” said Youmans.
The findings suggest that existing PRC2 inhibitors, while effective, may be working in a different way than previously believed. They also open the door for development of new drugs, which could get at the same end goal by preventing PRC2 from binding to certain locations on genes altogether.
“Cancer is always mutating so it’s important to have a broad toolbox of therapeutic options,” said Youmans.
As a student in CU’s Medical Scientist Training Program, which enables medical students to simultaneously pursue a
“I’m heartened by the idea that the work I do in the laboratory could ultimately help the patients I see in the hospital,” he said.