Below is a list to the current operational research cores supported by the School of Medicine and the research leadership on the Anschutz Medical Campus. The first group includes highly functional, well-established cores that are well-positioned to provide high quality and cost-efficient services to the research community. The second group encompasses emerging cores that have been developed in response to campus needs.
Admin in Charge: Tim Lockie, CCTSI, Administrative Director
Faculty in Charge: Pamila Allen & Karen Morgenthaler, Laboratory Supervisors
Services Provided: Chemical and Immunological Assays (University of Colorado Hospital, Children's Hospital Colorado), Processing of Sutum, Tracheal Spirates, and BAL fluids (Children's Hospital Colorado), Analysis of Human Inflammatory and Immunologic Response (National Jewish Health)
CTRC Core Laboratory Services support IRB-approved protocols with a wide range of analytical testing including chemical, hormone, metabolite, and immunological assays. CTRC Core Laboratories are CAP- and CLIA-accredited and provide trained personnel, reagents, equipment, and QC capabilities to conduct assays for research studies.
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Location: RC1N-Vivarium; P18-0437, P18-0434; RC2, P15-1109
Admin in Charge: Michaela Montour
Faculty in Charge: Natalie Serkova, PhD, Director
Services Provided: Small Animal Imaging (MRI/PET/CT Services, Optical Imaging Services, Metabolics Services)
Oncologic imaging is one of the fastest developing areas in cancer research because it answers multiple mechanistic and translational questions by noninvasive means and often in real time. The Animal Imaging Shared Resource (AISR) provides CU Cancer Center investigators access to state-of-the-art instrumentation and expertise in small animal imaging systems to facilitate non-invasive imaging based research. We offer MRI, PET, CT and bioluminescence imaging. In addition, NMR-based metabolomics and irradiation services are available. We provide our users with the established Standard Operating Procedures (SOPs) for all imaging-related animal handling and help in submitting to the IACUC.
AISR is the only facility in Colorado that provides comprehensive expertise in small animal imaging. Our goal is to facilitate all aspects of pre-clinical and translational cancer research by providing technical and scientific expertise in the area of cutting-edge imaging. This will facilitate the initiation of high-quality animal cancer research by CU Cancer Center members. AISR is strongly supported by the University of Colorado Cancer Center (UCCC), Colorado CTSA (CCTSI) and the Department of Anesthesiology.
Location: Building 500 6th N6201
Faculty in Charge: Dexiang Gao, PhD, Director (Biostatistics)
Faculty in Charge: James Costello, PhD, Co-Director (Bioinformatics)
Services Provided: Consultation on study design (clinical and basic science, including gene expression and proteomics experiments), Consultation on sample size and power, Development of data collection, storage, and quality control procedures (basic science and clinical studies, protocol review and monitoring), Data analysis, including genomic, metabolomic, and proteomic data. Data analysis pipelining, processing, and visualization. Collaboration on manuscript and oral presentation preparation, and grant proposal development.
Location: Leprino Building, Third Floor, CTRC Clinic
Admin in Charge: Tim Lockie, CCTSI, Administrative Director
Faculty in Charge: Kerrie Moreau, PhD
Services Provided: Vascular Imaging, Cardiac Imaging, Technique Development, Education
The Cardiovascular BioImaging Core at the University of Colorado Anschutz Medical Campus is a dedicated research facility for the acquisition and analysis of state of the science ultrasound imaging of the heart and vasculature. The Core allows researchers the ability to comprehensively assess vascular and cardiac health using the most advanced ultrasound and echo technology available. Investigators will also benefit from a centralized imaging processing center.
Location: Building B406 12477 E. 19th Ave., First Floor, 101
Admin in Charge: John Neal, Program Manager
Faculty in Charge: Nichole Carlson, PhD, Director
Services Provided: Bistatistics Consulting, Grant Collaboration
We provide statistical collaboration and resources to CCTSI investigators and trainees. We facilitate clinical and translational research through:
-Statistical consultation and collaboration
-Improved statistical literacy through seminars and short courses
-Providing access to advanced statistical tools
The BERD is collaboratively administered by CCTSI, Colorado Biostatistics Consortium (CBC), and the Children's Research Institute (RI). The CBC is home to 18 PhD, MS and graduate student biostatistics, bioinformatics, and health outcomes scientists. The CBC will collaborate on any aspect of your medical or health research project/grant. We develop collaborative agreements with groups for FTE, and assist with grant submission and budgeting of analytic support in grants. Data analysis for short term projects is done on a cost basis. The expertise is broad, including RNA seq, methylation, proteomics, metabolomics (broadly 'omics analysis), microbiome, survival analysis, clinical trials, Bayesian analysis, spatial modeling, longitudinal, electronic/administrative health records data, among many others. To request an initial consult, register at cbc.ucdenver.edu (prior to our name change - coming soon).
Faculty in Charge: Janine Higgins, PhD, Nutrition Research Director
Services Provided: Dietary intake assessment – habitual and during protocol interventions, Hunger and satiety assessment and/or measured ad libitum feeding, Meal preparation, including meal design, preparation, measurement, and dispensation, Growth, body composition, and resting metabolic rate (RMR) measurements in children, Consultation on study design, American dietary norms, and ways to achieve specific dietary intervention targets, Protocol-specific dietary counseling and instruction
Provides nutrition research expertise and assistance for clinical research protocols. We have two groups of professionals: Scientists and nutritionists with extensive experience in nutrition and metabolism research; Food service staff trained to prepare and distribute meals from our commercial kitchen.
Only CTRC-approved protocols can request Nutrition Services. All services are provided as fee-for-service resources.
Location: RC1-N (P18) Fourth Floor, RC 1 Vivarium Rooms 411C, 411D, 411E, 400B, 436, 438
Admin in Charge: Sandra Logue
Faculty in Charge: Amy Brooks-Kayal, MD, and Tim Benke, MD, PhD (Co-Directors); Michael Mesches, PhD, and Yodendra Raol, PhD (Supervisors)
Services Provided: The EEG core facility provides continuous rodent behavioral (video) and neurophysiological/EEG monitoring services (including cerebral electrical patterns during waking and sleep) to researchers. This type of monitoring will permit investigators to more thoroughly phenotype the rodents they use in their research and specifically address issues of whether they have abnormalities of neurophysiological functioning such as interictal epileptiform discharges, sleep disturbances and seizures. This can be important for the characterization of translational models of nervous system disorders, including stroke, epilepsy, head trauma, neurodegenerative, psychiatric, genetic and developmental disorders. The EEG Core, located in the RC1 Laboratory Animal Facility, has dedicated procedure room for stereotaxy and space for video-EEG monitoring. The core has the capability to video-EEG monitor 32 rats and 20 mice simultaneously using Stellate (Natus Medical Incorporated, San Carlos, CA) Pinnacle (Pinnacle Technology Inc., Lawrence, KS) and Triangle (Triangle BioSystems International, Durham, NC) equipment. The core is equipped with inhalation anesthesia systems, and digital, motorized, computer controlled stereotactic apparatus for electrode placement and cerebral injections in rat and mouse brain. The EEG core users will have free access to Review Stations located on the 3rd floor of the School of Pharmacy to review and analyze data. The review computers are loaded with Stellate, Pinnacle and Lab Chart software.
Location: RC1-S (L18) Seventh Floor, 7212
Admin in Charge: Uma Pugazhenthi; Audrey Wen
Faculty in Charge: Bryan Haugen, MD, Director
Services Provided: RNA isolation from tissues, cultured cells, blood, fine needle aspiration biopsies and LCM samples, Primer design and optimization, Quantitation of gene expression at the mRNA level, DNA copy number assay, Micro RNA quantitation, SNP genotyping, Pathway-specific gene expression profiling, ChIP assay end points, Plate run (Use of PCR machine), Measurement of absolute telomere length, qPCR genotyping assay for Down Syndrome mouse model Ts65Dn
The quantitative PCR core facility in the division of Endocrinology at UCDenver has been in operation since 1998 as a service facility to more than 100 investigators on and off campus. The core facility uses a laser based ABI 7900 Real time PCR instrument. Acknowledged as the gold standard in q-PCR, the 7900 system combined with the TaqMan genomic assays provides high throughput and flexibility, allowing users to pursue projects beyond the scope of any other Real time instrument. In collaboration with the Animal facility on campus, highly experienced core personnel have designed and optimized assays to detect sensitive levels of Corynebacterium bovis. The dedicated core lab space utilizes sterile equipment and aseptic techniques to provide quality data with a level of certainty that is useful for diagnostic research. Furthermore, an efficient turnaround time of 3-4 business days avoids potential delays in data progression.
Location: RC1-S (L18) 4th Floor, 4215, 4208, 4204
Admin in Charge: Karen Helm, MT(ASCP), Manager
Faculty in Charge: Eric Clambey, PhD, Director
Services Provided: Flow and Mass Cytometry Analysis, Cell Sorting, Luminex System, Vicell Cell Counter
Using flow or mass cytometric analysis, high-speed cell sorting, and multiplexed fluorescent microsphere assays, the Flow Cytometry Shared Resource (FCSR) can perform a wide range of assays supporting cancer research, including cell cycle, cell proliferation, apoptosis, cell viability, cell signaling, stem cell detection, fluorescent protein analysis and cell phenotyping. These assays are crucial to understand how cancer derails normal cellular processes to cause disease.
Also serves as the Flow Cytometry Core for the Gates Stem Cell Center and the CU Skin Diseases Research Cores. We provide a consultation to help those with little flow cytometry or immunology background to design appropriate experiments.
Location: RC1-N (P18) Sixth Floor 6222, 6223
Admin in Charge: Molishree Joshi, PhD, Manager
Faculty in Charge: Joaquin Espinosa, PhD, Director
Services Provided: shRNA, ORF, CRISPR, Cell Lines
The goal of the Functional Genomics Shared Resource (FGSR) is to promote scientific research in Colorado through the use of Functional Genomics, and catalyze discoveries that will positively impact the quality of human life. The FGSR administers genome-wide libraries of lentiviral vectors driving expression of shRNAs to knockdown gene activity in human and mouse cells, ORFs to overexpress human genes and gRNA constructs to knockout human genes. The facility also offers various custom services to build custom plasmids and plasmid-based libraries compatible with other species, and offers technical support for performing complex genetic screens. The core also provides consultation, and design and cloning support for gene-editing and gene-tagging experiments.
Location: RC1-N (P18) Eighth Floor 8403A
Admin in Charge: Brian A. Ward
Faculty in Charge: Maranke Koster, PhD
Services Provided: Paraffin and OCT embedding, Sectioning of Frozen and Paraffin Blocks, Routine (H&E) and Special Staining for all Types of Tissues
Our mission at the Morphology and Phenotyping Core is to provide investigators and their research teams with the tools, knowledge, and technical expertise needed to process and analyze their tissue samples. We offer a wide array of services including paraffin and OCT embedding, sectioning of frozen and paraffin blocks, as well as routine (H&E) and special staining for all types of tissues. Other histological services are available upon request. Finally, consultations are available to investigators as necessary.
Location: RC2 (P15) Ninth Floor 9400
Admin in Charge: Katrina Diener, Manager
Faculty in Charge: Bifeng Gao, PhD, Director
Services Provided: DNA Next-Gen Sequencing, DNA Microarray Analysis, Single-Cell Genomics, Proteomics, DNA/RNA Quantity and Quality Analysis, DNA/RNA Shearing, Pre-Experimental Design, Technology and Data Analysis Consultation
The Genomics and Microarray Core at the University of Colorado Denver is a genomic and proteomic facility providing access to Next Generation Sequencing, DNA microarray, single-cell genomics, and SomaLogic proteomic technology for the University and surrounding research institutions. We also assist with experimental design consultations and data analysis. An integral part of the core is the formal relationship with the University Bioinformatics Core to offer customers data analysis using novel algorithms.
Faculty in Charge: Dara Aisner, MD, PhD, Director
Services Provided: Pre-Analytical Evaluation and Molecular Testing (PEMT)
The PEMT service offers clinical-grade sample processing and molecular testing on pre-clinical models and clinical specimens. These services are provided to Cancer Center members for research purposes, while technical elements are performed in a clinical laboratory which regularly provides molecular testing services to patients.
The service ensures the use of adequate and accurately classified tumor and ancillary specimens for molecular testing. Sample processing including macro- and micro-dissection followed by nucleic acid extraction is provided. Molecular tests performed include several single-gene assays (Sanger sequencing, allele-specific qPCR, sizing), micro-satellite instability (MSI) testing, and targeted next-generation sequencing (NGS) assays for both gene mutations and gene fusions. All services are performed in a CLIA-certified environment and all molecular assays are accompanied with full data analysis (including bioinformatic analysis for NGS) and a complete report.
Location: National Jewish Health, 1400 Jackson St. D202, Denver, CO 80206
Admin in Charge: Shenee Taylor
Faculty in Charge: Jennifer Matsuda, PhD
The Mouse Genetics Core Facility supports investigators in all aspects related to the creation, maintenance and rederivation of novel mouse models. In essence, the facility has expertise in the following areas:
• construct generation: BAC recombineering, markerless recombineering, and cloning for BAC transgenics, classic trasngenics, targeting constructs, CRISPR/Cas9 HDR templates, expression and viral plasmids
• ES cell targeting and screening by LOA
• Pronuclear injections
• Blastocyst injections
• Embryo and sperm cryopreservation
• IVF and rederivation
• Design and generation of mice using engineered nucleases such as CRISPR/Cas9, including sequence verification of founders and screening for off target events
• Breeding to establish germline transmission
Our services have recently been further improved upon by our capacity to make mouse models directly on a variety of backgrounds, including but not limited to: C57BL/6, FVB, NOD, and NSGS backgrounds as well genetically modified strains of mice.
Locations: RC1-N (P18) Seventh Floor 7402H
Admin in Charge: Robby Rigby
Faculty in Charge: Abby Person/Gidon Felsen/Angie Ribera
Services Provided: Design and fabrication of light sources and delivery devices for in vivo/vitro optical stimulation, Consultation on adopting/optimizing the use of optogenetic methods, Design and production of custom hardware and software components, supply equipment to assist optogenetic experimentation, data analysis of neuronal experiments, engineering support for research realization, including use of 3D Printer.
The goal of the Optogenetics and Neural Engineering (ONE) Core is to facilitate the incorporation of optogenetics methods and custom engineering into existing research programs at the University of Colorado School of Medicine.
Location: Colorado State University - Flint Animal Cancer Center
Faculty in Charge: Dan Gustafson, PhD, director
Services Provided: Analyte Quantitation, Compound or Metabolite Identification, Pharmacology Study Support, Pharmacokinetic and Pharmacodynamic Modeling, Physiological Based Pharmacokinetic Modeling, Population Pharmacokinetic Modeling, Metabolism Studies, Drug Transporter Studies
The mission of PharmSR is to assist in the prospective design of studies to assess drug exposure in biological systems, to measure drug levels using validated analytical assays, and to analyze, model and interpret the results. Additionally, the PharmSR is part of the newly formed Colorado Center for Drug Discovery, a multi-institutional and multi-disciplinary drug development institute funded by the State of Colorado to facilitate drug development and discovery research programs at Colorado's academic institutions. The PharmSR serves as a core laboratory for this program as well as for the Colorado Clinical and Translational Sciences Institute (CCTSI)
Locations: RC2 (P15) Eighth Floor 8252, Vivarium
Admin in Charge: Kelley Brodsky
Faculty in Charge: Tim McKinsey, PhD, and Maria Cavasin, PhD
Services Provided: Small Animal Ultrasound, Small Animal Surgery, Small Animal Catheterization and Small Animal Organ Fibrosis Models
The core offers a wide variety of pre-clinical models in rats, mice and guinea pigs, from development to analysis. Our main therapeutic areas are cardiovascular, renal and pulmonary as well as the development of multiple organ fibrosis models . We utilize state-of-the-art data acquisition platforms, and offer a comprehensive suite of research services ranging from pharmacokinetics, target-based functional screens through model validation, and complex proof-of-concept studies.
Location: RC1-S (L18) Fifth Floor, 5404B
Admin in Charge: Lori Sherman, Manager
Faculty in Charge: Steven M. Anderson, PhD, Director
Services Provided: Protein Services (Mammalian System, Baculovirus System) and Monoclonal Antibody Services (Custom Hybridoma Services/Monoclonal Antibody Production), Cell Line Repository, General Cell Culture Services, Mycoplasma testing, cell line authentication service, on-site Corning Media Supply Center, and on-site pruchase of fetal calf, calf and other serum products. IncuCyte live cell imaging system available for investigators.
The Protein Production, Monoclonal Antibody, Tissue Culture Shared Resource (PPSR) supports basic and translational research by providing an on-campus repository of validated cell lines, cultivation of cells and by-products for investigators, preparation of hybridomas, production of monoclonal antibodies, isolation and titering of baculovirus expressing recombinant proteins, and large scale preparation of recombinant proteins and monoclonal antibodies. PPSR provides centralized services to produce proteins for biomedical researchers.
Location: Building 500, Ground Floor NG003
Admin in Charge: Robby Rigby
Faculty in Charge: Diego Restrepo, PhD, Michael Hall, Director
Services Provided: Machinery
The Neuroscience core machine shop is located in room NG003 on the ground floor of Building 500 (northwest corner) at the Anschutz Medical Campus (View Map). The shop's equipment and staff are there to meet the custom design and machining needs of all the faculty, staff and students of the UC Denver-Anschutz Medical Campus.
Locations: RC1-S (L18) 11th Floor 11402G
Faculty in Charge: Wallace Chick, PhD, Director
Services Provided: Transgenic (mouse), Viral (lenti, adenivirus, AAV, retro), Knock-Out/Knock-In
The core specializes in generating gene targeting and transgenic mouse models. The core has been serving the University of Colorado community since 2005. In that time, we have made over seventy vectors including knock-in, knock-out, conditional knock-out, traditional transgenic, and BAC transgenic. We have also developed specialized vector models for knock-in at the ROSA26 locus. For gene targeting models, our success rate for correct ES cell targeting is 95% and for germline transmission is 100%. The core also offers additional support services such as ES cell screening, BAC purification and mouse genotyping.
Location: RC1-N (P18) Seventh Floor, 7402C, 7402A1, 7402A2, 7206;
RC2 (P15) Seventh Floor, 7251, 7244; RC1-S (L18) 11th Floor, 11216
Admin in Charge: Radu Moldovan, PhD, Manager
Faculty in Charge: Mark Dell'Acqua, PhD, Director
Services Provided: Confocal Imaging, TPE/SHG Imaging, TIRF Imaging, Real-time Confocal Imaging, CARS Imaging, FCS/FCCS, RICS/N&B, Spectral Detection Imaging, FLIM, FRET Imaging, STED Super-Resolution Imaging, 3D STORM/PALM Super-resolution Imaging
The mission of the Advanced Light Microscopy Core (ALMC) is to provide CU-Anschutz investigators access to advanced fluorescence imaging research instrumentation at a reasonable price. The ALMC can assist you with fluorescent and label-free imaging of various cellular structures or tissues in several ways.
Location: RC1-N (P18) Fifth Floor 5208A, B, 5402L
Admin in Charge: Matthew Jackman, PhD, Assistant Director
Faculty in Charge: Paul MacLean, PhD, Director
Services Provided: Intake Monitoring, Indirect Calorimetry, Body Compostion (QMR, DEXA), Radiolabelled Dietary Tracer Studies, Mitochondrial/Cellular Respiration, Exercise Training, Consultation on Study Design and Analysis.
The Energy Metabolism Core and animal facilities provide support for PIs who want to measure energy intake, energy expenditure, physical activity, and body composition in small animals. This is the only metabolic phenotyping core on campus and the specialized equipment has been uniquely modified so that measures of energy balance can be acquired while assessing nutrient trafficking and metabolism with radioactive tracers. Although most of the work involves unique strains of rats and genetically modified mice, the equipment has also been used to study ground squirrels, pythons, and flies.
All services are provided as fee-for-service resources.
Location: RC1-S (L18) First Floor
Faculty in Charge: David Jones, PhD, Director
Services Provided: Structure Determination of Large Macromolecules (Proteins/Nucleic Acids), Crystallization Screening, Preliminary Crystal Screening and Data Collection, Solution Structural Analysis of Small Molecules, Molecular Visualization, Experimental Design and Training, Solution Screening for Molecular Interactions
The Structural Biology Shared Resource (SBSR) provides University of Colorado Cancer Center members access to instrumentation and expertise in the use of X-ray Crystallography and Nuclear Magnetic Resonance Spectroscopy (NMR). It is one of only two NCI-designated cancer centers with X-ray Crystallography, NMR, high field NMR and synchrotron. The SBSR promotes the application of structural studies to the molecular basis of cellular processes that regulate the development and progression of cancer and application of that information to aid in drug design, complementing the strengths of the existing research programs in cell signaling, pharmacology, and cancer.
Location: Biorepository: RC1-S (L18) Fifth Floor 5400A
Histology: RC1-N (P-18) 5th Floor 5404K
Admin in Charge: Managers: Histology: Erin Smith / Biorepository: Andrea Osypu
Faculty in Charge: Scott Lucia, MD. / Adrie van Bokhoven, PhD.
Services Provided: Biorepository
The Biorepository Core Facility supports research by insuring investigators have access to high quality tissue and body fluid specimens annotated with appropriate clinicopathologic and outcomes data. The Biorepository provides services in three main areas 1) procurement, processing, archiving, and distribution of fresh and formalin-fixed tissues and body fluids, 2) specimen characterization, quality control, and linkage with pathological and clinical annotation, 3) special processing and auxiliary services including full service histology lab, immunohistochemistry, tissue microarray construction, laser capture microdissection (LCM), nucleic acid isolation, and computer-assisted imaging/image analysis. The Biorepository Core Facility is fully capable of supporting the biospecimen components of any local or multi-institutional biobanking or clinical trial protocol and provide integration into the OnCore Clinical Trial Management and REDCap systems.
The Cancer Center Tissue Biobanking and Histology Shared Resource (TBHSR) and the CCCTSI Biobanking Consortium are managed as divisions under the University of Colorado Biorepository Core Facility. The Biorepository Core Facility has dedicated space for the freezer archive with 100 freezer bays and separate space for the formalin-fixed paraffin-embedded tissue archive that incorporates the surgical pathology archive containing specimens from diagnostic and surgical procedures. Incorporated Histology, LCM and imaging sub-cores are equipped with two Sakura VIP6 Tissue Processor, mcritomes, crystat, Leica H&E Autostainer, Ventana XT Immunostainer, Arcturus XT LCM instrument with IF and UV capabilities and Aperio Scanscope XT®.
Location: Barbara Davis Center, Fourth Floor 4201E
Admin in Charge: Allison Reeds
Faculty in Charge: Liping Yu, MD, Director
Services Provided: Diabetes autoantibodies, HLA typing
Autoantibody/HLA Service Core is a CLIA cerfied and NIH designated autoantibody/HLA reference laboratory for the North America. The Core provides the highest quality of services for diabetes autoantibody assays including autoantibodies to insulin (IAA), GAD65 (GADA), IA-2 (IA-2A), and ZnT8 (ZnT8A). The Core also provide the HLA typing including HLA class I (HLA-A and HLA-B) and HLA class II (HLA DRB and HLA DQA&B).
Location: RC1 South 11402J
Admin in Charge: Sandra Logue
Faculty in Charge: Wallace Chick, PhD
Services Provided: DNA Purification, RNA Purification, DNAse, Gene Expression, CNV
The Real-Time QPCR core was launched in February 2012 and has processed hundreds of samples for over 14 Principal Investigator;both within and outside of the university. The core provides real-time quantitative PCR services, including gene expression and copy number variation (CNV) analysis. Gene expression analysis is done using the relative standard curve method and an appropriate endogenous control. This ensures the most accurate data possible that will stand up to current publishing recommendations. Copy number analysis can be applied to test for transgene copy number in addition to inherent genomic variations. The core uses Applied Biosystems; 7500Fast instrument to deliver reliable data in a timely manner. The core also offers support services such as RNA and DNA purification and DNAse treatment.