Irradiation Services

The Radiation Sciences Core supports radiation biology studies of cell cultures and small animals receiving moderate-dose radiation. We also enable investigators to study low dose-rate irradiation in cell culture. And we provide irradiation services for investigators who need this tool in support of other efforts, such as using total body irradiation for immunosuppression of animals before stem cell transplantation.

Services We Offer:

  • Project design and consultation, including advice on radiation delivery to in vitro experiments and animal models
  • Design assistance for single or fractionated radiation protocols and study duration
  • Radiation safety training (all users) and radiation operation training (advanced users)
  • Animal tumor and partial-body irradiation at doses comparable to external beam radiotherapy
  • Cell suspension irradiation
  • High-dose irradiation to sterilize implant material
  • Dosimetry calculation
  • Calibration in support of stem cell research
  • Blocking cellular division in in vitro cell cultures
  • Immune function assays
  • Radiation-induced DNA damage repair studies
  • Data analysis an publication preparation assistance

Irradiator:

New Image-Guided Irradiator: Precision X-Ray X-Rad 225Cx Micro IGRT and SmART Systems, installed May 2017.

Contact: Sana Karam, MD, PhD or Ben VanCourt​

Prices:

The Radiation Sciences Core offers a discounted rate for CU Cancer Center members. Non-members are welcome to use our services as well, but we give members scheduling priority. We issue all users a key card that operates the equipment and tracks your time for easy billing.

Image-Guided Monitoring and Radiotherapy

The new established state-of-the art facility houses affordable, multi-modality imaging and precision radiotherapy tools for small animals. Image registration software allows accurate registration of images for longitudinal monitoring studies. Image registration between cone-beam computed tomography (CBCT) and bioluminescence imaging (BLI) provides an anatomical dimension to biological parameters that are luciferase tagged such as tumors. The high precision irradiator is equipped with collimators that provide accurate delivery of radiation to internal structures within 1mm accuracy. The versatile 360o rotation capability of the irradiator allows beam delivery through multiple angles, allowing delivery of high doses of radiation with minimal toxicity to normal tissue. The dose-volume histograms provide detailed information on the dose distribution within the target as well as outside the irradiated region. The high dose rate (6Gy/min) allows rapid delivery of the required dose in a short period of time. Animals can be anesthetized using the on-board isoflurane vaporizer ensuring safe delivery of the radiation and avoiding overdosing or underdosing of animals with injectable anesthetics.

Services Offered: 

  • Cone-beam CT imaging 
  • Image registration (within and between modalities)
  • Accurate and targeted delivery of ionizing radiation to any site 
  • Bioluminescence imaging
  • Radiotherapy experiment design
  • Data analysis

All services will be provided for $2/minute (same as RS2000 irradiator in RC-2​). This includes isoflurane anesthesia and animal setup. Luciferin can be provided for BLI at commercial prices.

Investigators performing complex procedures are encouraged to setup an initial appointment for protocol setup. For more information, or to make an appointment please contact Benjamin Van Court​.