Molecular and Cellular Analytic Core

About the MCA Core 


The overall aim of the Molecular and Cellular Analytical (MCA) core is to support the investigation of human and non-human metabolism from a cellular/molecular perspective. The core focuses on investigation in the following areas:

Lipidomics and Mass Spectrometry

GC/MS Analysis

Mitochondrial Respiration

Bioinformatic Analysis of RNAseq Data

Lipidomics and Mass Spectometry

Offering analytical services, as well as expertise in interpretation of lipidomic data, the Lipidomics/Mass Spectrometry services can quantitatively analyze the following lipids from plasma as well as cells and tissues (muscle, heart, liver, bone):

  • Triacylglycerols (TAGs) 
  • DAGs/Ceramides/Glycosphingolipids – diacylglycerols (DAGs), ceramides (Cer), dihydroceramides (dHCer), glucosyl ceramides (GluCer), galactosyl ceramides (GalCer), and lactosyl ceramides (LacCer)
  • Sphingolipids and Acyl Carnitines – sphingosine (SPH), acyl carnitines (AC), sphingomyelin (SPM)
  • Glycerophospholipids – includes phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), phosphatidylinositol (PI), phosphatidic acid (PA), phosphatidylglycerol (PG), and all lyso derivatives of these compounds
  • Eicosanoids – HETEs, HODEs, diHOMEs, diHETs (diHETrEs), leukotrienes, prostaglandins, thromboxane
  • Oxidized PCs – POVPC, PGPC, PONPC, and PAzPC

GC/MS Anlaysis

The Bergman Lab has been performing GC/MS analysis for ongoing research as well as collaborative projects since 2004. The lab analyzes plasma samples for concentration and enrichment of glucose, glycerol, and free fatty acids (FFA). The methods used include Glucose and glycerol: Penta-acetate derivatization in EI mode and FFAs: PFB derivatives in CI mode. 

Bioinformatic Analysis of RNAseq Data

Next-Gen Sequencing. Our purpose is to provide existing members the NORC and researchers at the University of Colorado Anschutz Campus with a consolidated workflow for the management, processing, and biological interpretation of unbiased RNA sequencing gene expression data.

Basics of the Service. We process high resolution RNA sequencing data (FastQ) and implement state-of-the-art computational and bioinformatics methods to supply the end user with statistically significant genes, annotated gene lists, canonical pathways, putative upstream regulators, disease states, gene interaction networks, and biological meaning specific to their research question.

Mitochondrial Function Sub Core - Mitochondrial Respiration

The Mitochondrial Function Sub Core of the MCA Core facilitates the comprehensive measurement of mitochondrial function and cellular bioenergetics for both academic and industry users by providing equipment for end users. Respirometry measurements may be performed on a host of biological samples including cultured/primary cells, permeabilized fibers or cells, and or isolated mitochondria.

Training and professional development in the use and understanding of the various respiration methodologies is provided. Basic training/consultation is routinely provided for use of the Seahorse XF96 Extracellular Flux Analyzer, and the BioTek Cytation instruments. More in depth training/consultation is provided for the Oroboros O2k, and Strathkelvin systems.

Mitochondrial Sub Core Equipment & Pricing

The following equipment is also available for study use on mitochondrial function if needed:

High speed refrigerated floor centrifuge

2 bench top centrifuges

Microfuges

Mettler semi-micro balances

Dissecting microscope


MCA Core Contacts


Director: Bryan Bergman, PhD

Associate Director: Kartik Shankar, PhD

Mitochondrial Sub Core: Matthew Jackman, PhD

Lipidomics and GS/MS Analysis: Karin Zemski-Berry, PhD

View the MCA Team

Colorado Nutrition Obesity Research Center (NORC)

CU Anschutz Health and Wellness Center

12348 East Montview Boulevard

Aurora, CO 80045


norc@cuanschutz.edu

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