Molecular and Cellular Analytic Core
About the MCA Core
The overall aim of the
Molecular and Cellular Analytical (MCA) core is to support the investigation of
human and non-human metabolism from a cellular/molecular perspective. The core
focuses on investigation in the following areas:
Lipidomics and Mass Spectrometry |
Lipidomics and Mass Spectometry
Offering analytical services, as well as expertise in interpretation of lipidomic data, the Lipidomics/Mass Spectrometry services can quantitatively analyze the following lipids from plasma as well as cells and tissues (muscle, heart, liver, bone):
- Triacylglycerols (TAGs)
- DAGs/Ceramides/Dihydroceramides – 1,3- and 1,2-diacylglycerols (DAGs), ceramides (Cer), dihydroceramides (dHCer)
- Sphingolipids and Acyl Carnitines – sphingosine (SPH), acyl carnitines (AC), sphingomyelin (SPM), ), glucosyl ceramides (GluCer), and lactosyl ceramides (LacCer)
- Glycerophospholipids – includes phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), phosphatidylinositol (PI), phosphatidic acid (PA), phosphatidylglycerol (PG), and all lyso derivatives of these compounds
- Cardiolipin (CL)
- Eicosanoids – HETEs, HODEs, diHOMEs, diHETs (diHETrEs), leukotrienes, prostaglandins, thromboxane
- Oxidized PCs – POVPC, PGPC, PONPC, and PAzPC
- Steroids
Lipidomic Analysis Equipment
ABSciex 3200 Acylcarnitines Sphingomyelins
ABSciex 4000 for TAGs and CL
DAG/Sphingolipids and Oxidized PC
ABSciex 7500 - steroids and eicosanoids
GC/MS Analysis
The Bergman Lab has been performing GC/MS analysis for ongoing research as well as collaborative projects since 2004. The lab analyzes plasma samples for concentration and enrichment of
- Glucose
- Glycerol
- Free fatty acids (FFA)
The methods used include:
- Glucose and glycerol: Penta-acetate derivatization in EI mode
- FFAs: PFB derivatives in CI mode
GCMS for glucose/glycerol and FFA
ThermoScientific Trace 1310
Bioinformatic Analysis of RNAseq Data
Next-Gen Sequencing
Our purpose is to provide existing members the NORC and researchers at the University of Colorado Anschutz Campus with a consolidated workflow for the management, processing, and biological interpretation of unbiased RNA sequencing gene expression data.
Basics of the Service
We process high resolution RNA sequencing data (FastQ) and implement state-of-the-art computational and bioinformatics methods to supply the end user with statistically significant genes, annotated gene lists, canonical pathways, putative upstream regulators, disease states, gene interaction networks, and biological meaning specific to their research question.
Mitochondrial Function Sub Core - Mitochondrial Respiration
The Mitochondrial Function Sub Core of the MCA Core facilitates the comprehensive measurement of mitochondrial function and cellular bioenergetics for both academic and industry users by providing equipment for end users. Respirometry measurements may be performed on a host of biological samples including cultured/primary cells, permeabilized fibers or cells, and or isolated mitochondria.
Training and professional development in the use and understanding of the various respiration methodologies is provided. Basic training/consultation is routinely provided for use of the Seahorse XF96 Extracellular Flux Analyzer, and the BioTek Cytation instruments. More in depth training/consultation is provided for the Oroboros O2k, and Strathkelvin systems.
Mitochondrial Sub Core Equipment & Pricing
| Current Pricing: | |
| XFe96 Seahorse | $119.76/run |
| Cytation-1 | $25/run |
| Oroboros O2k | $142.50/O2k/day |
| Protein Simple Jess | $30/run |
In order to schedule/reserve the equipment above, you will need an iLabs account which can be set up here: https://cu.corefacilities.org/account/login.
Once the iLabs account is obtained, please contact Matthew Jackman for access to the MCA core website reservations.
The following equipment is also available for study use on mitochondrial function if needed:
High speed refrigerated floor centrifuge | 2 bench top centrifuges | Microfuges | Mettler semi-micro balances | Dissecting microscope
MCA Core Contacts
Director: Bryan Bergman, PhD
Associate Director: Kartik Shankar, PhD
Mitochondrial Sub Core: Matthew Jackman, PhD
Lipidomics and GS/MS Analysis: Karin Zemski-Berry, PhD