Organoid and Tissue Modeling Shared Resource

Overview

The Organoid and Tissue Modeling Shared Resource (OTMSR) is an institutional, fee-for-service core facility supporting advanced organoid technologies on campus. The OTMSR provides researchers with access to state-of-the art organoid and gene editing technologies and expert technical support to utilize novel in vitro 3D organoid model systems for basic and translational research.

What are Organoids?

Organoids are defined as “self-organizing three dimensional (3D) structures grown from stem cells which mimic the in vivo architecture and multi-lineage differentiation of the original tissue.” Organoids can be derived from two types of stem cells: (i) pluripotent stem cells (PSCs) or (ii) tissue-derived somatic stem cells.

PSC-derived organoids recapitulate developmental programming and have the potential to form tissue/organ structures through processes that occur during development.

Tissue-derived somatic organoids recapitulate adult tissue repair mechanisms rather than development and in general, only tissues that exhibit some regenerative capacity are amenable to this approach.

Mission

Contact Us


Organoid and Tissue Modeling Shared Resource Core


Barbara Davis Center
1775 Aurora Court
M20 - 3202N
Aurora, CO 80045

Hours | 9:00 am - 5:00 pm

Lab Phone | 303-724-8235

The overall mission of the OTMSR core is to facilitate access, generation and usage of novel in vitro mouse and human organoid systems to promote innovative basic and translational research and disease modeling. To help overcome the obstacles faced by basic scientists and clinical researchers who wish to establish these approaches in their labs, we will provide expertise, resources and training in these start-of-the-art technologies together with standardization for protocols, subsidized reagents and validated organoid lines. This will be achieved in four ways: 

  1. Providing access and training in current and emerging organoid technologies for in vitro disease modeling
  2. Providing access, training and implementation of genome editing technology and gene expression systems
  3. Performing critical key quality control and assurance measures to assure the validation and authenticity of core-derived resources to rigorously meet and exceed emerging NIH guidelines
  4. Provide data sharing of all OTMSR-related resources, protocols and technologies to research community
  • Project Consultation
  • Experimental Design and Planning
  • Training
  • Protocols
  • Growth Factor Conditioned Media
  • Complete Organoid Media
  • Matrigel
  • Media Supplements
  • Recombinant Growth Factors
  • Reprogramming
  • Endodermal Organoid Programming
  • Gene modification
  • Validation and Authentication
  • Mouse Organoid Generation
  • Human Organoid Generation
  • Established Organoid Lines & Organoid Libraries
  • Gene Modification
  • Validation and Authentication Services
  • Gene editing
  • Gene expression
  • 3D Live and Fluorescent Imaging Microscopy
  • Morphometric and Image Analysis Work Station
  • Matrigel liquid handling
  • STR Analysis
  • Mycoplasma Testing

Core Staff

Name E-mail Phone
Peter Dempsey, PhD peter.dempsey@cuanschutz.edu 303-724-5602
Sean McGrath, PhD patrick.mcgrath@cuanschutz.edu 303-724-8235
Monica Brown monica.e.brown@cuanschutz.edu 303-724-8235 

iLabs (coming soon)

Useful Resources