Organoid and Tissue Modeling Shared Resource

Overview

The Organoid and Tissue Modeling Shared Resource (OTMSR) is an institutional, fee-for-service core facility supporting advanced organoid technologies on campus. The OTMSR provides researchers with access to state-of-the art organoid and gene editing technologies and expert technical support to utilize novel in vitro 3D organoid model systems for basic and translational research.

Currently, OTMSR provides organoid services to:

  • Cell Technologies Shared Resource from UC Cancer Center
  • Disease Modeling core from UC Diabetes Research Center
  • Organoid core from the UC Gates Center of Regenerative Medicine
  • Enteroid Stem Cell core, Gastrointestinal and Liver Innate Immunity (Galiip) program
OTMSR logo

What are Organoids?

Organoids are defined as “self-organizing three dimensional (3D) structures grown from stem cells which mimic the in vivo architecture and multi-lineage differentiation of the original tissue.” Organoids can be derived from two types of stem cells: (i) pluripotent stem cells (PSCs) or (ii) adult tissue-derived somatic stem cells.

Contact Us

Organoid and Tissue Modeling Shared Resource Core

Barbara Davis Center

PSC-derived organoids recapitulate developmental programming and have the potential to form tissue/organ structures through processes that occur during development.


Tissue-derived somatic organoids recapitulate adult tissue repair mechanisms rather than development and in general, only tissues that exhibit some regenerative capacity are amenable to this approach.

1775 Aurora Court
M20 - 3202N
Aurora, CO 80045

Hours | 9:00 am - 5:00 pm

Lab Phone | 303-724-8235

Mission

The overall mission of the OTMSR is to facilitate access, generation and usage of novel in vitro mouse and human organoid systems to promote innovative basic and translational research and disease modeling. To help overcome the obstacles faced by basic scientists and clinical researchers who wish to establish these approaches in their labs, we will provide expertise, resources, and training in these start-of-the-art technologies together with standardization for protocols, subsidized reagents and validated organoid lines. This will be achieved in four ways: 

  1. Provide access and training in current and emerging organoid technologies for in vitro disease modeling
  2. Provide access, training and implementation of genome editing technologies and gene expression systems
  3. Maintain and provide access to banked and fully-characterized organoid and PSC lines
  4. Implement critical quality control and assurance measures to ensure the validation and authenticity of core-derived resources rigorously meet and exceed emerging NIH guidelines
  5. Provide data sharing of all OTMSR-related resources, protocols and technologies to the research community
  • Project Consultation
  • Experimental Design and Planning
  • Training
  • Protocol Sharing
  • Growth Factor Conditioned Media
  • Complete Organoid Media
  • Matrigel
  • Media Supplements
  • Recombinant Growth Factors
  • Reprogramming
  • Endodermal Organoid Differentiation
  • Gene Modification
  • Validation and Authentication
  • Human and Mouse Organoid Generation
  • Ready-To-Purchase Organoid Lines & Libraries
  • Gene Modification
  • Validation and Authentication
  • Gene Editing
  • Gene Expression
  • Experimental Design & Optimization
  • 3D Live and Fluorescent Imaging Microscopy
  • Morphometric and Image Analysis Work Station
  • Mantis automated liquid handler medium throughput organoid assay development

Core Staff

Name E-mail Phone
Peter Dempsey, PhD peter.dempsey@cuanschutz.edu 303-724-5602
Sean McGrath, PhD patrick.mcgrath@cuanschutz.edu 303-724-8235
Monica Brown monica.e.brown@cuanschutz.edu 303-724-8235 

iLabs (coming soon)

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