Tissue-Derived Organoid Services

The OTMSR offers organoid generation services from primary mouse and human tissues, in addition to providing access to a variety of fully characterized mouse and human organoid libraries.

Generating Human Adult Tissue-Derived Organoids

Requirements: If tissue is to be provided by the interested investigator, IBC approval is required. To meet the appropriate verification requirements, there is a minimum amount of tissue required for all adult tissue-derived organoid generations.

Process: Tissue is processed according to established OTMSR protocols which vary depending on tissue type, disease status, and known mutational profiles. Generation can be tailored to fit the investigator’s needs and skillsets.

New Organoid Models: To establish a new organoid model the OTMSR does not have an established protocol for, an existing published protocol is required and must be provided by the interested investigator.

Generating Mouse Adult Tissue-Derived Organoids

Requirements: Tissue must be collected by the investigator per IACUC approval. Training is available to ensure proper collection per the OTMSR’s protocol requirements.

Process: Tissue is processed according to established OTMSR protocols which can vary depending on tissue type. Generation can be tailored to fit the investigator’s needs and skillsets.

New Organoid Models: To establish a new mouse organoid model the OTMSR does not have an established protocol for, an existing published protocol is required and must be provided by the interested investigator.

Organoid Lines and Libraries

The OTMSR has generated numerous human and mouse organoid lines which are available to investigators. All available lines go through basic authentication and validation and are free of mycoplasma.

 Examples of Organoid Lines:

                Origin organ – include pancreas, colon, region-specific small intestine etc.

                Disease status – include normal, tumor etc.

                Mutational status – KRAS mutant, p53 mutant etc.

*A comprehensive list of available organoid lines is available after initial consultation.

Validation and Authentication Services

All available organoid lines have passed a variety of quality control measures including mycoplasma testing, species identification, and STR analysis.

Species identification, mycoplasma testing, STR analysis, and Sanger sequencing services are offered through the Molecular Biology Core.