The objective of the BDC Molecular Biology Core Laboratory is to facilitate and promote the application of contemporary molecular biological techniques to existing research programs at University of Colorado Anschutz Medical Campus, its affiliated institutions and outside entities. Investigators in our Research Base come from diverse clinical and scientific backgrounds including immunology, epidemiology, genetics, surgery, developmental biology, biochemistry, cellular biology, and molecular biology. The Core provides researchers with up-to-date and innovative molecular biology techniques which include:
• Capillary electrophoresis applications on ABI 3730 and 3500xL instruments
o Automated DNA sequencing
o Automated fragment analysis (Cell line authentication and Zygosity testing)
• Mycoplasma testing
• gDNA extraction and purification from cell pellets
• RNA quality and quantification using an Agilent 2100 Bioanalyzer
• Dynamic glucose stimulated insulin secretion via perifusion
The core has multiple dropoff locations around the Anschutz Medical Campus for your convenience. Sample collection occurs at 11AM Monday thru Friday (Holidays excepted).
• RC1 South 8th floor- in the box near the elevators
• Barbara Davis Center 1st floor - on the windowsill, just inside the first set of doors of the Northwest entrance, near the screening table
• Barbara Davis Center, submit them directly in the lab or leave at 4˚C in the molecular core refrigerator (white tube rack labeled “customer sample drop-off, room 4207”)
Automated DNA Sequencing
Mycoplasma Testing
Interspecies and Cross Contamination Testing
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