The objective of the BDC Molecular Biology Core Laboratory is to facilitate and promote the application of contemporary molecular biological techniques to existing research programs at University of Colorado Anschutz Medical Campus, its affiliated institutions and outside entities. Investigators in our Research Base come from diverse clinical and scientific backgrounds including immunology, epidemiology, genetics, surgery, developmental biology, biochemistry, cellular biology, and molecular biology. The Core provides researchers with up-to-date and innovative molecular biology techniques which include:
• Capillary electrophoresis applications on ABI 3730 and 3500xL instruments
o Automated DNA sequencing
o Automated fragment analysis (Cell line authentication and Zygosity testing)
• Mycoplasma testing
• gDNA extraction and purification from cell pellets and FTA cards
• Interspecies and Cross Contamination Testing (Human, Rat, and Mouse)
• DNA and RNA quality and quantification using an Agilent 2100 Bioanalyzer
The core has multiple dropoff locations around the Anschutz Medical Campus for your convenience. Sample collection occurs a 1PM Monday thru Friday (Holidays excepted).
• RC1-South, 8th floor – near elevator area
• RC1-North, 4th floor – just inside the lab hall near the elevators. On the right, there is a labeled plastic container.
• RC2, 9th floor – just inside the lab hall near the elevators. On the left, there is a labeled plastic container.
• Barbara Davis Center, 4th floor – Executive office area
• Barbara Davis Center, submit them directly in the lab or leave at 4˚C in the molecular core refrigerator (white tube rack labeled “customer sample drop-off, room 4207”)
Automated DNA Sequencing
- Dye-terminator sequencing on ABI 3130x1 Genetic Analyzer capillary instrument
- 800-1000bp Q20 read length
- Online sample submission and results retrieval
- Drop Box Locations at RC1-N, RC1-S, RC2
- Next business day results for samples submitted before 1pm
- Free reruns
- See Sample Submission Guidelines for details and requirements
- List of Primers available provided by the MBCF
- Sanger Sequencing trouble shooting guides can be found here and here.
Human Cell Line Authentication
- Human Identification STR Analysis (Zygosity testing, human cell line authentication)
- For ID testing, 15ul gDNA template at >30ng/ul are required
- Please see our STR Analysis and Cell Line Authentication page
- PCR based detection of various mycoplasma species
- 50-100ng of genomic DNA for Sample Submission
- We utilize the e-Myco PLUS PCR Mycoplasma Detection Kit
- For Mycoplasma testing, 15ul gDNA template at >30ng/ul are required
Interspecies and Cross Contamination Testing
- Multiplex PCR assay that will screen for different species contamination in your cell lines
- Will detect for the presence of human, mouse, rat, rabbit, and Chinese hamster
- gDNA sample submitted for STR testing or mycoplasma testing can be used
- 15ul gDNA template at >30ng/ul are required
- 'Notice Regarding Authentication of Cultured Cell Lines' from NIH.
Barbara Davis Center University of Colorado, Anschutz Medical Campus 1775 Aurora Court, 4th floor, Room 4207 Phone: 303-724-6825Molecular Biology contact personnel: Mark Farrell
, Core Manager