The MBCF performs the service of Human Identity testing for the purposes of zygosity testing and cell line authentication (CLA). We utilize the STR DNA Profiling Globalfiler PCR Amplification Kit from Thermo Fisher.
The MBCF provides STR profiling for the purposes of CLA for customers that wish to confirm the identity of a cell line. Identity can be ‘authenticated’ by comparison to a known cell line DNA profile or by comparing allelic information and matching/confirming identity with published information, commercial reference information, or a reference sample from the donor. The process of donor origin authentication is a multifaceted process with many elements of data analysis to reach a final conclusion. In an effort to aid users in implementation of the most current accepted methods and best scientific practices, we’ve assembled a list of very informative links below with very detailed information about utilizing your DNA ‘fingerprint’ once you’ve received your report from us. Alternatively, you can request that we perform the authentication analysis and provide you with a concise summary report of your tested sample.
Two STR Testing Services are available-
Cell Line Authentication Testing Guidelines/Recommendations
Authentication testing at key intervals of a project should be performed when you-1. Establish or acquire a new cell line.
3. Are routinely passaging cell lines.
5. Prepare to publish or submit grant application.
6. Freeze cell stocks.
Links for STR Cell Line Authentication Resources and Documentation
Implementing Rigor and Transparency in NIH & AHRQ Research Grant Applications.
NIH Notice regarding CLA.
ICLAC Guide to CLA.
ICLAC Advice to Scientists.
ATCC Cell Line Authentication Test Recommendations.
Guidelines for the Use of Cell Lines in Biomedical Research.
Match criteria for human cell line authentication: Where do we draw the line?
Recommendation of short tandem repeat profiling for authenticating human cell lines, stem cells, and tissues.
Short tandem repeat profiling: part of an overall strategy for reducing the frequency of cell misidentification.
ATCC resources page- scroll down to CLA links.
BioSample- List of published cell line data.
Promega CLA reference List.
Mycoplasma Testing for Biologics from WuXi AppTec.
Molecular Biology contact personnel: