Organoid and Tissue Modeling Shared Resource
iPSC-Derived Organoid Services
Human induced pluripotent stem cells (hPSCs) have the intrinsic potential to form any cell type and have proven to be an important tool for basic and translational research. The OTMSR provides access to high-quality hPSCs for experimental use. Additionally, the OTMSR can perform directed differentiations to select endodermal lineages and provide 3D organoids for further research.
Human Induced Pluripotent Stem Cells (hiPSCs)
The OTMSR can supply fully validated control hiPSCs for your experimental needs.
hiPSC Line | Sex | Patient Age | Race | Primary Cell Source | Reprogramming Method | Notes |
iCT1 | male | neonatal | Caucasian | dermal fibroblast | mRNA | |
| iCT2 | male | 66y | Caucasian | dermal fibroblast | mRNA | |
| iCT3 | female | 36y | Caucasian | dermal fibroblast | mRNA | |
| iCT4 | female | 45y | Black | dermal fibroblast | mRNA | Available HLS typing results |
*Quality control data available upon request (pluripotency marker expression, hPSC morphology, karyotype analysis).
hiPSC differentiation to endodermal lineages
The OTMSR can provide differentiated human intestinal organoids and pancreatic beta-like cells for investigator use.
Gene Modification
The OTMSR offers comprehensive hiPSC genome modification using CRISPR/Cas9.
- Targeted gene knock-outs
- Fluorescent knock-ins
- Point mutations
hiPSC Reprogramming Services
Comprehensive reprogramming services are provided by the Stem Cell Biobank and Disease Modeling Core for the derivation of hiPSCs from primary patient samples. Reprogrammed hiPSCs can then be received by the OTMSR for further genetic modification and/or directed differentiation.