We study how the expression of genetic information is spatially regulated within a cell. Individual mRNA molecules are often trafficked to specific cellular locations. This facilitates robust, localized protein production where and when it is needed. Although thousands of mRNAs are asymmetrically distributed in cells, the RNA sequences and protein factors that regulate this process are unknown for the vast majority of messages. We use experimental and computational methods to understand mechanisms behind this regulation and how disruption of the process can result in neurological disease.