Ph.D., Purdue University, 1998
Phone: (303) 724-3246
The Eisenmesser lab takes a unique approach to understand protein function, and particularly enzyme function, by utilizing molecular engineering methods to control both structural interactions and the underlying movements that underlie their conformational changes. The ultimate goal of the Eisenmesser lab is to fully characterize molecular interactions at both atomic resolution and biological levels with a particular emphasis on medically relevant systems that may be exploited to either block or promote events underlying disease progression. For example, we utilize NMR, X-ray crystallography, and cryo-EM methods together with biochemical and cellular biological studies to fully characterize molecular events that underlie molecular interactions involved in signaling and cellular homeostasis. Our approaches go well beyond static descriptions of molecular complexes to include the role of dynamics both within enzyme active sites and allosterically coupled movements that modulate function. In regard to enzyme movements, our studies have been cited as “the first attempt to rationally engineer enzyme motions” (see Peptide Letters, Doucet, 2011) and provide a critical means of unraveling how enzyme movements have evolved to perform specific tasks.
The current focus of the Eisenmesser lab are three projects described in what follows, which focus on:
Interleukins: Interleukins (ILs) are a major class of cytokines responsible for regulating the innate immune response and their deregulation has been shown to occur during inflammation, cancer, and infection. The Eisenmesser lab is particularly focused on understanding the signaling events utilized by anti-inflammatory interleukins such as IL-37 in order to modulate their structure and biological activities and use as potential therapeutics. Such studies began during the PI’s postdoctoral work on IL-13 but have since been extended to other interleukins that include IL-8 and the anti-inflammatory interleukins that include IL-37 and IL-38.
Toll-Interleukin receptor (TIR) domains: TIR domains are conserved domains found within both interleukin-1 family receptors (IL-1Rs) and Toll-like family receptors, yet despite the 20 years since their discovery, their molecular interactions have eluded direct detection. The Eisenmesser lab is particularly focused on understanding how these TIR domains bridge signaling at the membrane surface with downstream interactions of adaptor TIR domains. While we are interested in determining the molecular interactions that occur at atomic resolution for pro-inflammatory TIRs, such as TLR1/2, we are also interested in understanding how IL-1R family receptors, such as those that include IL-1R8 that work to downregulate inflammation. Finally, we are also interested in understanding how bacteria utilize their own TIR domains to control host cell immunity through host cell TIR mimicry.
Our interest in human infection and developing strategies to specifically block infection has fostered efforts at understanding how bacterial subvert our initial immune response. CryoEM and NMR studies are focused on determining the molecular mechanisms of giant proteases that have multiple domains, such as the Streptococcus pneumonia IgA1 Protease (IgA1P). These IgA1Ps represent a diverse array of virulence factors that are examples of convergent evolution, as their IgA1P regions are metalloproteases, serine proteases, and cysteine proteases. Many IgA1Ps from different pathogenic bacteria comprise additional domains of unknown function beyond their IgA1P regions. For example, we have solved the structures of small G5 domains from an IgA1P through NMR to begin identifying its ligands. However, our central focus is to elucidate the atomic-resolution details of their IgA1P regions using cryoEM, as illustrated here by our recent elucidations of the ~1300 residue Streptococcus pneumonia IgA1P alone and during catalysis of its IgA1 substrate that indicates a “pakman” like mechanism of active site opening (or “gating”).
We have made several major contributions to the field of protein dynamics, which have had direct implications for understanding of protein function, allostery, and have now allowed us to engineer enzyme dynamics in a rational way. The Eisenmesser lab is particularly interested in the interplay between structure and the dynamics that underlie conformational rearrangements of enzymes to direct their function. Our recent studies have focused on a key flavin reductase family of enzymes responsible for modulating cellular redox, called Biliverdin Reductase B (BLVRB), which are so important that they control cellular fate. We have discovered that enzyme dynamics modulate function up to 25 angstroms from the active site in human BLVRB, but we are focused on combining NMR and X-ray crystallography to determine how global motions modulate function in the entire family and how these motions have evolved. Such studies have revealed novel mechanisms of coenzyme regulation, revealing that coenzyme release is the rate-limiting step and that the single hydride modulates the global dynamic and structure.
The collaborative environment within the Department of Biochemistry & Molecular Genetics has fostered many collaborations, which include those with Dr. Hongin Zheng, Dr. Kirk Hansen, Dr. Angelo D’Alessandro, Dr. Rui Zhao, Dr. Jeff Kieft, and Dr. James DeGregori. For example, multiple ongoing projects with the D’Alessandro lab are focused on key metabolic interactions governed by enzymes and we are highly focused on producing these enzymes to identify their atomic resolution details that govern their functions.