Typically ~4 weeks from the time we receive the samples. For multiomics submissions, turnaround times may be closer to 5-6 weeks. Please feel free to inquire by email at any time during this timeframe for a more precise estimate specific to your project.

Relative quantification yields unitless peak area measurements. When plotting these, the most common units to include are “au” or “arb” for arbitrary.

These are injections of a pooled QC sample (single vial) performed at the beginning, end, and periodically throughout the sequence to assess instrument performance and signal stability.

These are injections of our metabolite extraction solution at the beginning and end of the sequence to measure the background signal at each m/z.

This is the coefficient of variation (i.e. relative standard deviation) for the tech mix injections across the sequence. A lower value indicates less peak area variation, therefore greater signal stability.

Not directly. Peak area is a function of a compound’s concentration and its ionizability, an inherent chemical property that describes how well the structure stabilizes a positive or negative charge. Ionizability is influenced by chemical structure and the complexity of the sample matrix/background. As a result, two metabolites with the same concentration will yield different peak areas. Raw peak areas can be compared for a metabolite across the sample set but not across metabolites.

No, this process requires internal or external standards with data acquired in the same batch as your experimental samples.

MS acquisition is subject to significant batch effects occurring with day to day variation of signal background. Thus we recommend that all samples that are to be directly (quantitatively) compared be analyzed in the same batch.

MetaboAnalyst: data normalization, PCA/PLS-DA, p-values, volcano plots, heat maps, biomarker analysis, pathway analysis, correlations

Morpheus: heat maps