The primary mission of the Anschutz Cryo-EM Facility and its personnel is to enable efficient collection of high-quality EM data to advance molecular EM projects on the Anschutz Medical School campus and beyond. Involvement of facility personnel in areas beyond data collection (e.g., specimen preparation, specimen optimization, image analysis) will be limited by time and resource availability.
Basic training on automated data collection and use of equipment for cryo-EM sample preparation is considered part of the Facility’s services. Any project in which facility personnel enables anything beyond data collection (e.g., prepare samples, optimize sample preparation conditions, any analysis of resulting image data) will be considered a collaboration.
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Data was collected on a 200 kV ThermoFisher/FEI Talos Arctica microscope with a K3 Summit direct electron detector (Gatan) (or on a 120 kV ThermoFisher/FEI Talos L120C microscope with a Ceta CMOS detector). Consult Facility Personnel for relevant additional information specific to individual data collection runs.
Indicate that data was collected at the Anschutz Medical Campus Cryo-EM Core Facility and acknowledge help from the Anschutz Cryo-EM Core Facility staff.
Cryo-EM Core Facility personnel should be listed as co-authors in any publications resulting from collaborative work (as described above). Methods and Acknowledgements as above.
Please send a link to the paper and a representative figure to the director of the Anschutz Cry o-EM Core Facility
All common EM image processing packages (Relion, CryoSPARC, SPARX, Freealign, cisTEM, SPHIRE, EMAN, etc.), as well as map analysis and modeling software (Coot, PHENIX, Chimera, etc.) are installed in the core’s GPU workstations. Access to computing resources is generally limited to Anschutz Medical School researchers and their collaborators.