Policies and Procedures
Prior to submitting samples, please refer to the CU Proteomics Core Sample Submission Guidelines and fill out the Proteomics Sample Submission Form.
Submitting samples to this core implies acceptance of the terms of service stated below in the Authorship and Grant Assistance sections.
Once submitted, you will receive a confirmation email with instructions for scheduling sample dropoff. The Proteomics Core Facility accepts samples from all CU Anschutz researchers and from external users, as capacity permits.
Please contact the facility before you send any samples to discuss your needs and to determine the best approach to analyze your samples. The most important goal is for us to get a solid understanding of your aims and expectations. Don't hesitate to contact us with any questions.
Please note that it is very difficult to estimate the total cost of a custom service until a number of factors are known. This includes, but is not limited to:
- How much method development and validation is required?
- The complexity of the assay (number of manipulations, instrument time and supplies needed, difficulty of data analysis).
- Core Facility instrumentation used
- Number of analytes measured
- Cost/availability of internal or external standards.
We will clearly communicate cost via email prior to analysis and are glad to provide formal quotes upon request.
Customers internal to CU must provide a speed type for invoice payments. Those outside of CU may pay by check, credit card or wire transfer.
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For questions regarding payment options, please contact Sarah Vinton.
After analysis, you will receive all data in Excel spreadsheet format (.xlsx). This tabular data will contain protein and peptide identifications reported as both peptide spectral matches (PSMs) and AUC intensity. Data can also be provided in Scaffold (Proteome Software) by request.
Additional data analysis, including figure generation and statistical analyses, can be performed for an additional cost of $120 per hour of analysis. Bioinformatics can also be performed through the Biostatistics and Bioinformatics Shared Resource (BBSR).
RAW data will be stored on in-house file servers for and maintained for at least one year from the date of acquisition. If longer-term storage is required, RAW data files can be provided via Dropbox upon request. Alternatively, RAW files can be submitted to the ProteomeXchange Consortium online data repository for a fee (please inquire for details).
Payment of fees and authorship are not mutually exclusive. When authorship guidelines are met it will be necessary for core members who have performed the analysis to be recognized as co-authors.
Co-authorship is generally expected when core personnel have made significant contributions to the research in the form of consultation, experimental design, method development, data analysis and/or data interpretation. Significant is defined as "the project would not have progressed, or progressed at a substantially slower pace, without the guidance of facility personnel." In such cases, core personnel should have the opportunity to review and edit the appropriate sections of a manuscript before submission.
When the conditions of co-authorship are not met, acknowledgement of facility contributions is expected in publications that include data generated in the facility or processed using core instrumentation or software. An example of appropriate acknowledgement is "The authors wish to thank the University of Colorado School of Medicine Biological Mass Spectrometry Proteomics Core Facility for their contributions to this manuscript."
Please inform us when relevant publications are accepted, as we post citations on our website and in our annual reports. This information is vital to the continued support of the facility.
How to Cite RRID Information
University of Colorado Shared Resources (SR) are supported by the National Cancer Institute through the Cancer Center Support Grant (P30CA06934).
Guidelines:
- Any published or presented work accomplished using shared resources is required to acknowledge the shared resource with the following statement in the acknowledgments: “This study was supported in part by the National Institutes of Health P30CA06934 funded [fill in name] Shared Resource [RRID (see list below)].”
- When available during journal submission, identify the Cancer Center Support Grant (P30CA06934) as an NIH source of support for the research.
- Any publications resulting from the use of shared resources MUST be filed with PubMed Central and should be linked to the Cancer Center Support Grant (P30CA046934; PI: Richard Schulick MD) through MyNCBI.
- Instructions for submitting your publication to PubMed Central can be found here: NIH Public Access Policy.
Grant Assistance and Letters of Support
We are very happy to provide letters of support and assistance in grant writing. For letters of support, please complete our LOS request form and allow at least 2 weeks form the time of your request.
Our facility is also dependent on grant support for long-term success and we appreciate your support in keeping the facility funded.
To determine if percent effort by core personnel is required, consider the following:
- Does the proposal include a specific aim(s) that focuses on proteomics?
- Is method development required?
- Is data analysis development required?
- Would the aim or proposal not receive a favorable review without the support of Core personnel?
- Would the project not have progressed, or progressed at a substantially slower pace, without the guidance of facility personnel?