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Project Submission Guidelines
Experimental Design
For pilot experiments, single replicates can be run to assess potential differences.
For publication experiments, 3+ biological replicates required for statistical analysis. 6 replicates is ideal for improved statistical power.
Sample Amount
Tissue Samples
3-5 mg dry weight (15- 20 mg wet weight) is sufficient, but larger samples are preferred if available.
Cell Cultures
1-2 million cells per sample is ideal; 200k is generally sufficient.
Biofluids
A minimum of 50-100 uL is required
Sample Preparation
Tissue Samples
Freeze in pure/untreated form
Cell Cultures
Freeze cell pellets in 1.5-2 mL Eppendorf MS-compatible microcentrifuge tubes devoid of all supernatant
Biofluids
Freeze in pure/untreated form
Sample Submission Process
- Contact core personnel ( Anthony Saviola) to schedule sample drop-off.
- Complete sample submission form.
- Please provide samples frozen in 1.5-1.7 mL labeled microcentrifuge tubes (no screw caps).
- Samples must be in a standard size paper freezer box labeled with submitter name, PI name, submission date, and project identifier from sample confirmation.
- Please email electronic sample list (xlsx) to Anthony Saviola.
- Bring box of samples on dry ice to the Proteomics Core (RC1-S, first floor, room 1303) during the following times:
If you do not have RC1-S access, please call the core (303-724-3339) before leaving your lab so that we can meet you outside.
Sample Shipment Guidelines
- Contact core personnel ( Anthony Saviola) to coordinate sample shipping.
- Complete sample submission form.
- Please provide samples frozen in 1.5-1.7 mL labeled microcentrifuge tubes (no screw caps).
- Please package on dry ice and include a hard copy of the sample list that is labeled with submitter name, PI name, and project identifier from sample confirmation.
- Please email electronic sample list (xlsx) to Anthony Saviola.
- Please overnight on Monday or Tuesday to the below address. If shipping from outside North America, please ship on Friday or Monday.
SOM Proteomics Core
University of Colorado Anschutz Medical Campus
12801 E 17th Ave, Room 1303
Aurora, CO 80045 USA
Phone 1-303-724-3339
What to Expect (Deliverables)
After analysis, you will receive all data in Excel spreadsheet format (.xlsx). This tabular data will contain protein and peptide identifications reported as both peptide spectral matches (PSMs) and AUC intensity. Data can also be provided in Scaffold (Proteome Software) by request.
Additional data analysis, including figure generation and statistical analyses, can be performed for an additional cost of $120 per hour of analysis. Bioinformatics can also be performed through the Biostatistics and Bioinformatics Shared Resource (BBSR).
Contact
For project inquiries and consultations please contact the team at
somproteomics@cuanschutz.edu