Metabolomics

NMR-Based Services

Metabolomics, one of the “omic” sciences in systems biology, is the global assessment and validation of endogenous small-molecule biochemicals (metabolites) within a biologic system.

The Animal Imaging Shared Resources Core (AISR) provides all NMR-related metabolomics services on human and animal cells, biopsies, and body fluids. 

The facility is fully equipped for all aspects of nuclear magnetic resonance spectroscopy (1H-,
13C-, and 31P-NMR and two-dimensional NMR). We perform expanded quantitative metabolic analysis on:

• Cells
• Cell extracts
• Human and animal tissues and biopsy extracts
• Body fluids (including blood, plasma, urine, cerebral and prostatic fluids etc).
  

Our Services

• Project design and consultation
• Sample Handling and Extraction for Metabolic NMR
• NMR based metabolic protocols
• Data set collection is available.
• Data analysis and publication preparation/ assistance.
• Data handling and storage

Sample Handling & Protocols:

We will provide all users with carefully designed and validated protocols for sample handling and sample extraction.

Please contact Jenna Steiner, AAS, CVT, to schedule services and obtain the following protocols:

• Dual methanol/chloroform blood extraction protocol
• Dual methanol/chloroform tissue extraction protocol 
• Acid Tissue Extraction protocol 
• Acid Cell Extraction 
• Quantitative 1H-, 31P- and 13C-MRS