Molecular Pathology (RRID: SCR_021995)

The Molecular Pathology Section offers clinical-grade sample processing and molecular testing for research cases.  All services are performed in a CLIA-certified environment and all molecular assays are accompanied by full data analysis (including bioinformatics analysis for NGS data)

Services

• Pathologist examination of tissue samples
• Sample microdissection for internal testing 
• Sample microdissection to release extract for external testing
• 69-gene next-generation sequencing (NGS) mutation assay
• 53 gene NGS gene fusion assay  
• Single gene mutation/sequence analysis (Sanger, qPCR, sizing)
• 24 locus STR analysis for identity confirmation and mixed sample analysis
• Microsatellite instability testing 
• MGMT & MLH1 promoter hyper-methylation testing
• Custom assay design and evaluation
• Testing for common polymorphisms in Factor 2, 5, HFE
• Huntington Disease testing
• Cystic fibrosis gene sequence by NGS
• Digital droplet PCR (ddPCR) analysis of select targets

All services include

• ​Experimental design consultation
• A full analysis of all data (including bioinformatics analysis for NGS data)
• An easy to interpret final report

Equipment

• Nanodrop and Qubit nucleic acid quantitation systems  
• Agilent Bioanalyzer and TapeStation nucleic acid analysis systems  
• Pipetting robot  
• Thermocyclers  
• Roche Lightcycler for quantitative PCR and RT-PCR analysis  
• Qiagen Rotor-Gene Q instrument for qPCR  
• Olympic microscopes, photomicroscopic equipment, and Leica microdissection scopes  
• ABI 3500xl DNA analyzer  
• Three Illumina MiSeq NGS sequencers  
• Illumina NextSeq (for upcoming expanded NGS assays)
• Biocartis Idylla
• BioRad QX200 Droplet Digital PCR System

Fees are available upon request. Please contact Kurtis Davies, PhD for more information.