The Cittelly laboratory studies the mechanisms that allow breast cancer cells to metastasize to the brain, with long-term goal of identifying alternative treatment options for patients with this -often terminal- diagnosis. Brain metastases develop in a unique organ comprised by specialized groups of cells that control brain function in a tightly regulated manner. Intrinsic tumor genetics as well as specific signals within the brain niche contribute to the outgrowth of disseminated cancer cells. Our group uses in vitro and in vivo approaches to elucidate the role of glial cells (reactive astrocytes and microglia) and neurons to brain metastatic progression, defining how hormones alter the neuro inflammatory milieu through metastatic colonization, and how the brain niche contributes to failure of standard of care for brain metastasis (i.e Radiation, targeted therapies).
Young women with breast cancer have increased risk of developing brain metastases independent on the primary tumor subtype. We have shown that ovarian hormones, particularly estradiol, promotes brain metastatic colonization and progression by acting on estrogen-responsive cells within the brain niche. We have identified at least two of these mechanisms (upregulation of BDNF and EGF in astrocytes, which in turn activate TRKB and EGFR in cancer cells), leading to increased invasion and tumor-initiating ability. Current studies aim to: 1) Define whether FDA-approved drugs targeting TRK/EGFR can prevent or treat brain metastases; 2) Define the transcription factors and molecular mechanisms driving the adaptation of tumors cells to the brain niche in response to E2;
;3) Define the role of ERα and ERb in reactive astrocytes in mediating estrogen signaling during brain metastatic progression (using genetically-engineered mouse models and pharmacologic approaches);
While it is well-known that metastatic colonization requires cells to change between highly invasive phenotypes (for extravasation) to proliferative phenotypes (for outgrowth); the mechanisms driving this switch in the brain remain unclear. We have shown that IL13RA2 promotes this phenotypic switch trough repression of Eph-EphrinB axonal guidance system (EFNB1), in a ligand-independent manner. Current studies in our group aim to: 1. Determine how IL13RA2 and Eph/ephrinB1 drives proliferation-invasion switch in cancer cells, 2. Define interaction of IL13RA2 with ligands in the TME contribute to brain metastatic progression
The majority of patients with brain metastasis are treated with radiation (stereotactic radiosurgery-SRS) as part of their standard of care. However, radiation induced toxicity (necrosis and edema) has emerged as critical side effect, particularly for patients receiving certain combinations of targeted therapies for their tumors. We have identified dysregulation of water-channels APQ4 in astrocytes, as one mediator of cytotoxic edema in the brain following SRS. Current projects further define how radiation alone or in combination with standard of care for breast cancer influence the development of radiation-necrosis and edema.
In collaboration with breast oncologists and neurosurgeons at University of Colorado AMC, we have developed xenograft models derived from breast cancer brain metastases from all breast cancer subtypes. We have engineered a protocol for manipulation of gene expression in these PDXs, including expression of optical labels for in vivo tracking, which allow us to model spontaneous and experimental metastasis of these PDXs in vivo. We are combining these with our spontaneous and experimental metastasis models in transgenic mice, to mechanistically evaluate the interactions between cancer cells and specific cells within the brain microenvironment during metastatic progression.
1. Sartorius CA, Hanna CP, Gril B, Cruz H, Kabos P, Serkova NJ, Huber KM, Schedin TB, Borges VF, Steeg PS, Cittelly DM. Estrogen promotes the brain metastatic colonization of triple negative breast cancer via an astrocyte-mediated paracrine mechanism. Oncogene. 2016; 35(22):2881-92. PMID: 26411365
2. Hanna CT, Kwok L, Finlay-Schultz J, Sartorius CA, Cittelly DM. Labeling of breast cancer patient-derived xenografts with traceable reporters for tumor growth and metastasis studies. 2016. Jove. 2016; (117). PMID: 27929464.
3. Contreras-Zárate MJ, Ormond DR, Gillen AE, Hanna C, Day NL, Serkova NJ, Jacobsen BM, Edgerton SM, Thor AD, Borges VF, Lillehei KO, Graner MW, Kabos P, Cittelly DM. Development of Novel Patient-Derived Xenografts from breast cancer Brain metastases. Front Oncol. 2017 Nov 2; 7:252. doi: 10.3389 /fonc.2017.00252. eCollection 2017. PMCID: PMC5673842
4. Contreras-Zárate MJ, Day NL, Ormond DR, Borges VF, Tobet S, Gril B, Steeg P, Cittelly DM. Estradiol induces BDNF/TrkB signaling in triple-negative breast cancer to promote brain metastases. Oncogene. 2019 https://doi.org/10.1038/s41388-019-0756
5. Stumpf PK*, Cittelly DM*, Robin TP, Carlson JA, Sthr KA, Contreras-Zárate MJ, Lai S, Ormond DR, Rusthoven CG, Gaspar LE, Rabinovithc R, Kavanagh B, Liu A, Diamond JR, Kabos PK, Fisher CM. Combination of trastuzumab emtansine and stereotactic radiosurgery results in high rates of clinically significant radionecrosis and dysregulation of Aquaporin-4. Clin Cancer Res. 2019 Apr 2. pii: clincanres.2851.2018. doi: 10.1158/1078-0432.CCR-18-2851. *Co-first authors.
6. Marquez-Ortiz RA, Contreras-Zárate MJ, Tesic V, Alvarez-Eraso KL, Kwak G, Littrell Z, Costello JC, Sreekanth V, Ormond DR, Karam SD, Kabos P, Cittelly DM*. IL13Rα2 promotes proliferation and outgrowth of breast cancer brain metastases. Clin Cancer Res. 2021 Sep 20:clincanres.0361.2021. doi: 10.1158/1078-0432.CCR-21-0361.
The Cittelly Lab is looking for creative thinkers, innovators, and problem solvers. People who can see the “big picture” while being meticulous about details. People who makes good use of their time and lab resources and is committed to the success of the lab as it is their own. People who are respectful, professional and enjoy working in the lab and with others. Brain metastasis remain a critical need in basic and translational research and we are looking to build the next generation of brain metastases scientists! Please contact Diana.Cittelly@cuanschutz.edu for more info.
-BS, Biology, Universidad de Columbia (1998)
-MS, Biochemistry, Universidad Nacional de Colombia (2001)
-PhD, Cell Biology, University of Texes Medical Branch (2007
Honors and special recognition (selected):
-2014 - Keystone Symposia on Molecular and Cellular Biology, 2014-2015 Fellows program
-2019- Invited faculty expert to Annual Biomedical Research Conference for Minority Students (ABRCMS), Anaheim, CA Nov 13-16th
-2018- Recipient of R37 MERIT Award NCI
-2022- Recipient of DoD BCRP Level II breakthrough Award