Molecular and Cytogenetics
Cytogenetics Service (RRID: SCR_021991)
The University of Colorado Cancer Center Molecular Pathology Shared Resource, in collaboration with Colorado Genetics Lab, provides cytogenetic and cytogenomic research services. Services include tissue culture, chromosome analysis, fluorescence in situ hybridization (FISH), and chromosomal microarray. We also offer the design and development of customized FISH probes. We are available for consultation on study design and data analysis.
Services
- Cell line characterization by FISH or karyotype analysis.
- Chromosome and karyotype analysis for human and murine specimens.
- Fluorescence in situ hybridization (FISH) studies for gene mapping, structural rearrangement, and copy number changes.
- For a full list of available probes click here.
- Customized FISH probe development.
- Chromosomal microarray (CMA) analysis of human cells or tissues for genomic copy number changes.
- Consultation on study design and data analysis.
Fees are available upon request. Please contact Hala Nijmeh, PhD for more information.
Molecular Pathology (RRID: SCR_021995)
The Molecular Pathology Section offers clinical-grade sample processing and molecular testing for research cases. All services are performed in a CLIA-certified environment and all molecular assays are accompanied by full data analysis (including bioinformatics analysis for NGS data)
Services
- Pathologist examination of tissue samples
- Sample microdissection for internal testing
- Sample microdissection to release extract for external testing
- 69-gene next-generation sequencing (NGS) mutation assay
- 53 gene NGS gene fusion assay
- Single gene mutation/sequence analysis (Sanger, qPCR, sizing)
- 24 locus STR analysis for identity confirmation and mixed sample analysis
- Microsatellite instability testing
- MGMT & MLH1 promoter hyper-methylation testing
- Custom assay design and evaluation
- Testing for common polymorphisms in Factor 2, 5, HFE
- Huntington Disease testing
- Cystic fibrosis gene sequence by NGS
- Digital droplet PCR (ddPCR) analysis of select targets
All services include
- Experimental design consultation
- A full analysis of all data (including bioinformatics analysis for NGS data)
- An easy to interpret final report
Equipment
- Nanodrop and Qubit nucleic acid quantitation systems
- Agilent Bioanalyzer and TapeStation nucleic acid analysis systems
- Pipetting robot
- Thermocyclers
- Roche Lightcycler for quantitative PCR and RT-PCR analysis
- Qiagen Rotor-Gene Q instrument for qPCR
- Olympic microscopes, photomicroscopic equipment, and Leica microdissection scopes
- ABI 3500xl DNA analyzer
- Three Illumina MiSeq NGS sequencers
- Illumina NextSeq (for upcoming expanded NGS assays)
- Biocartis Idylla
- BioRad QX200 Droplet Digital PCR System
Fees are available upon request. Please contact Kurtis Davies, PhD for more information.
Contact Us
Cytogenetics
Mary Haag, PhD
Associate Director
Pathology Shared Resource
Cytogenetics Section Leader
Hala Nijmeh, PhD
Manager
Cytogenetics Section
p | 303-724-8063
Molecular Pathology
Dara Aisner, MD, PhD
Associate Director
Pathology Shared Resource
Molecular Pathology Section Leader
Kurtis Davies, PhD
Manager Molecular Pathology
p | 303-724-9257
Main Email
Location
BioScience 2, Suite 400
12705 E. Montview Blvd.
Aurora CO, 80045