Topic 5


Development of New HPLC and Capillary Electrophoresis Methodology

The development of new separation methodology is critical to research in academia, biotechnology and pharmaceutical sectors. A few examples of new methodology developed in our laboratory are listed below: a new mixed-mode hydrophilic/cation-exchange chromatographic method for the separation of peptides that rivals reversed-phase chromatography (RPC) where selectivity is completely different; a new capillary electrophoresis method called “Ion-interaction capillary zone electrophoresis” which separates not only by charge/ mass ratio but by subtle differences in hydrophobicity as well; a novel method to measure self-association of small amphipathic molecules referred to as temperature profiling in RPC; a one-step purification of recombinant proteins from a whole cell extract by RPC; and we have determined the intrinsic hydrophilicity/hydrophobicity of amino acid side-chains in peptides in the absence of nearest-neighbor or conformational affects and showed that this scale was the most accurate determination of side-chain hydrophilicity/hydrophobicity and of general value to all fields of biomedical science.

Selected publications are listed.

Mant, C.T. and R.S. Hodges. Separation of peptides on HALO 2-micron particles. Current Protocols in Protein Science 85: 11.6.1-11.6.16 (2016). doi:10.1002/cpps.12 (Wileyonlinelibrary.com, John Wiley & Sons, Inc.).

Mant, C.T., Z. Jiang, B. E. Boyes and R.S. Hodges. An improved approach to hydrophilic interaction chromatography of peptides: Salt gradients in the presence of high isocratic acetonitrile concentrations. J. Chromatography A 1277: 15-25 (2013). PMC3639484 PMID23332786

Mant, C.T. and R.S. Hodges. Design of peptide standards with the same composition and minimal sequence variation to monitor performance/selectivity of reversed-phase matrices. J. Chromatography A , 1230: 30-40 (2012). PMC3294100 PMID22326185

Mant, C.T., D. Cepeniene and R.S. Hodges. Reversed-phase HPLC of Peptides: Assessing column and solvent selectivity on standard, polar embedded and polar end-capped columns. J. Separation Science 33:3005-3021 (2010). No PMC PMID21038458

Mant, C.T., J.M. Kovacs, H.M. Kim, D.D. Pollock and R.S. Hodges. Intrinsic amino acid side-chain hydrophilicity/hydrophobicity coefficients determined by reversed-phase high-performance liquid chromatography of model peptides: comparison with other hydrophilicity/hydrophobicity scales. Biopolymers (Peptide Science) 92: 573-595 (2009). PMC2792893 PMID19795449​

Publications

2015-2019

  1. Mant, C.T. and R.S. Hodges. Separation of peptides on HALO 2-micron particles. Current Protocols in Protein Science 85: 11.6.1-11.6.16 (2016). doi:10.1002/cpps.12 (Wileyonlinelibrary.com, John Wiley & Sons, Inc.).

2010-2014

  1. Huang, Y.B., L. Pan, L.J. Zhao, C.T. Mant, R.S. Hodges and Y. Chen. Structure-guided RP-HPLC chromatography of diastereomeric α-helical peptide analogs substituted with single amino acid stereoisomers. Biomed. Chromatogr. 28: 511-517 (2014). No PMC PMID24127254 DOI 10.1002/bmc.3061
  2. Zajdowicz, S., J.C. Haller, A.E. Krafft, S.W. Hunsucker, C.T. Mant, M.W. Duncan, R.S. Hodges, D.N.M. Jones, and R.K.Holmes. Purification and Structural Characterization of Siderophore (Corynebactin) from Corynebacterium diphtheriae. PLoS one 2012; 7(4) e34591. PMC3326035 PMID22514641
  3. Mant, C.T. and R.S. Hodges. Design of peptide standards with the same composition and minimal sequence variation to monitor performance/selectivity of reversed-phase matrices. J. Chromatography A , 1230: 30-40 (2012). PMC3294100 PMID22326185
  4. Mant, C.T., D. Cepeniene and R.S. Hodges. Reversed-phase HPLC of Peptides: Assessing column and solvent selectivity on standard, polar embedded and polar end-capped columns. J. Separation Science 33:3005-3021 (2010).

2005-2009

  1. Mant, C.T., J.M. Kovacs, H.M. Kim, D.D. Pollock and R.S. Hodges. Intrinsic amino acid side-chain hydrophilicity/hydrophobicity coefficients determined by reversed-phase high-performance liquid chromatography of model peptides: comparison with other hydrophilicity/hydrophobicity scales. Biopolymers (Peptide Science) 92: 573-595 (2009).
  2. Mant, C.T. and R.S. Hodges. Mixed-mode hydrophilic interaction/cation exchange chromatography: separation of complex mixtures of peptides of varying charge and hydrophobicity. J. Separation Science 31: 1573-1584 (2008).
  3. Mant, C.T. and R.S. Hodges. Mixed-mode hydrophilic interaction/cation exchange chromatographic (HILIC/CEX) separation of peptides and proteins. J. Separation Science 31: 2754-2773 (2008).
  4. Tripet, B., D. Cepeniene, J.M. Kovacs, C.T. Mant, O.V. Krokhin and R.S. Hodges. Requirements for prediction of peptide retention time in reversed-phase high-performance liquid chromatography: hydrophobicity/hydrophilicity of side-chains at the N- and C-termini of peptides are dramatically affected by end-groups and location. J. Chromatography A 1141: 212-225 (2007).
  5. Chen, Y., C.T. Mant and R.S. Hodges. Preparative reversed-phase high performance liquid chromatography of an antimicrobial peptide on columns of varying diameters (1 mm to 9.4 mm). J. Chromatography A 1140: 112-120 (2007).
  6. Popa, T., C.T. Mant and R.S. Hodges. Ion-Interaction Capillary Zone Electrophoresis: The presence of high concentrations of ion-pairing reagents demonstrates the complex mechanisms involved in peptide separations. Special issue on Capillary Electrophoresis (CE) and Capillary Electrochromatography (CEC) of Amino Acids, Peptides and Proteins. Electrophoresis 28:2181-2190 (2007).
  7. Tripet, B., M. RenukaJayadev, D. Blow, C. Nguyen, R.S. Hodges and K.J. Cios. Proteomic Data Mining Using Predicted Peptide Chromatographic Retention Times. The International Journal of Bioinformatics Research and Applications. IJBRA. 3:431-445 (2007).
  8. Mant, C.T., Y. Chen, Z. Yan, T.V. Popa, J.M. Kovacs, J.B. Mills, B. Tripet and R.S. Hodges. HPLC Analysis and Purification of Peptides. In “Peptide Characterization and Application,” Methods in Molecular Biology Series (Ed. G.B. Fields) Humana Press 386: 3-55 (2007).
  9. Mant, C.T. and R.S. Hodges. Context-dependent effects on the hydrophilicity/hydrophobicity of side-chains during reversed-phase high-performance liquid chromatography: implications for prediction of peptide retention behavior. J. Chromatography A 1125: 211-219 (2006).
  10. Mills, J.B., C.T. Mant, and R.S. Hodges. One-step purification of recombinant proteins from a whole cell extract by reversed-phase high-performance liquid chromatography. J. Chromatogr. A. 1133: 248-253 (2006).
  11. RenukaJayadev, M., D. Blow, B. Tripet, R.S. Hodges and K.J. Cios. Proteomic Data Mining Using Predicted Peptide Chromatographic Retention Times. Proc. of the Biotechnology and Bioinformatics Symposium (BIOT-06), Brigham Young University, Provo, Utah, October 20-21; 84-89 (2006).
  12. Kovacs, J.M., C. T. Mant, D. J. Osguthorpe, S.C. Kwok, R. S. Hodges. Quantitation of the nearest-neighbour effects of amiono acid side-chains that restrict conformational freedom of the polypeptide chain using reversed-phase liquid chromatography of synthetic model peptides with L- and D- amino acid substitutions. J. Chromatography A 1123: 212-224 (2006).
  13. Kovacs, J.M., C.T. Mant and R.S. Hodges. Determination of the intrinsic hydrophilicity/hydrophobicity of amino acid side-chains in peptides in the absence of Nearest-Neighbor or Conformational Effects. Peptide Science (Biopolymers) 84: 298-309 (2006).
  14. Popa, T.V., C.T. Mant and R.S. Hodges. Ion interaction capillary zone electrophoresis (II-CZE) of cationic proteomic peptide standards. J. Chromatography A 1111: 192-199 (2006).
  15. Shibue, M., C.T. Mant and R.S. Hodges. Effect of anionic ion-pairing reagent hydrophobicity on selectivity of peptide separations by reversed-phase chromatography. J. Chromatography A 1080: 68-75 (2005).
  16. Shibue, M., C.T. Mant and R.S. Hodges. Effect of anionic ion-pairing reagent concentration (1 mM – 60 mM) on reversed-phase chromatography elution behavior of peptides. J. Chromatography A1080: 58-67 (2005).
  17. Shibue, M., C.T. Mant and R.S. Hodges. The perchlorate anion is more effective than trifluoroacetate anion as a ion-pairing reagent for reversed-phase chromatography of peptides. J. Chromatography A 1080: 49-57 (2005).

2000-2004

  1. Hodges, R.S., Y. Chen, E. Kopecky, and C.T. Mant. Monitoring the hydrophilicity/hydrophobicity of amino acid side-chains in the non-polar and polar faces of amphipathic a-helices by reversed-phase and mixed-mode hydrophilic interaction/cation-exchange chromatography. J. Chromatography A 1053: 161-172 (2004).
  2. Popa, T.V., C.T. Mant, Y. Chen and R.S. Hodges, Capillary zone electrophoresis (CZE) of a-helical diastereomeric peptide pairs using anionic ion-pairing reagents. J. Chromatography A 1043: 113-122 (2004).
  3. Chen, Y., A.R. Mehok, C.T. Mant and R.S. Hodges. Optimum concentration of trifluoroacetic acid (TFA) for reversed-phase chromatography of peptides revisited. J. Chromatography A 1043: 9-18 (2004).
  4. Popa, T.V., C.T. Mant and R.S. Hodges. Capillary electrophoresis of cationic random coil peptide standards: effect of anionic ion-pairing reagents and comparison with reversed-phase chromatography. Electrophoresis 25: 1219-1229 (2004).
  5. Chen, Y., C.T. Mant and R.S. Hodges. Selectivity differences in the separation of amphipathic a-helical peptides during reversed-phase chromatography at pH 2.0 and pH 7.0: effects of different packings, mobile phase conditions and temperature. J. Chromatography A 1043: 99-111 (2004).
  6. Popa, T.V., C.T. Mant and R.S. Hodges. Capillary electrophoresis of amphipathic a-helical peptide diastereomers. Electrophoresis 25: 94-107 (2004).
  7. Hartmann, E., Y. Chen, C.T. Mant, A. Jungbauer and R.S. Hodges. Comparison of reversed-phase liquid chromatography and hydrophilic interaction/cation-exchange chromatography for the separation of amphipathic a-helical peptides with L- and D-amino acid substitutions in the hydrophilic face. J. Chromatography A. 1009:61-71 (2003).
  8. Mant, C.T., Y. Chen and R.S. Hodges. Temperature profiling of polypeptides in reversed-phase liquid chromatography: I. Monitoring of dimerization and unfolding of amphipathic a-helical peptides. J. Chromatography A. 1009:29-43 (2003).
  9. Mant, C.T., B. Tripet and R.S. Hodges. Temperature profiling of polypeptides in reversed-phase liquid chromatography: II. Monitoring of folding and stability of two-stranded a-helical coiled-coils. J. Chromatography A. 1009:45-59 (2003).
  10. Lee, D.L., C.T. Mant and R.S. Hodges. A novel method to measure self-association of small amphipathic molecules: Temperature profiling in reversed-phase chromatography. J. Biol. Chem.278: 22918-22927 (2003).
  11. Chen, Y., C.T. Mant and R.S. Hodges. Temperature selectivity effects in reversed-phase liquid chromatography due to conformation differences between helical and non-helical peptides. J. Chromatogr. A. 1010:45-61 (2003).
  12. Popa, T.V., C.T. Mant and R.S. Hodges. Capillary electrophoresis of synthetic peptide standards varying in charge and hydrophobicity. Electrophoresis 24:4197-4208 (2003).
  13. Mant, C.T. and R.S. Hodges. Reversed-phase liquid chromatography as a tool in the determination of the hydrophilicity/hydrophobicity of amino acid side-chains at a ligand-receptor interface in the presence of different aqueous environments: I. Effect of varying receptor hydrophobicity. J. Chromatogr. A 972: 45-60 (2002).
  14. Mant, C.T. and R.S. Hodges.Reversed-phase liquid chromatography as a tool in the determination of the hydrophilicity/hydrophobicity of amino acid side-chains at a ligand-receptor interface in the presence of different aqueous environments: II. Effect of varying peptide ligand hydrophobicity. J. Chromatogr. A 972: 61-75 (2002).
  15. Mant, C.T. and R.S. Hodges. Preparative reversed-phase chromatography of proteins from a multi-protein complex: rabbit skeletal troponin. J. Chromatogr. A 972: 101-114 (2002).
  16. Mehok, A.R., C.T. Mant, L. Gera, J. Stewart and R.S. Hodges. Preparative reversed-phase chromatography of peptides: isocratic two-step elution system for high loads on analytical columns. J. Chromatogr. A 972: 87-99 (2002).
  17. Mant, C.T. and R.S. Hodges. Analytical HPLC of Peptides. In "HPLC of Biological Macromolecules: Methods and Applications" (Second Edition) (K. Gooding and F. Regnier, Eds). Marcel Dekker, Inc. New York, N.Y. pp 433-511 (2002).
  18. Mant, C.T. and R.S. Hodges. Liquid Chromatography [Hydrophilic interaction/cation-exchange chromatography of peptides]. In Encyclopedia of Separation Science (Academic Press), Editors I.D. Wilson, T.R. Adland, C.F. Poole and M. Cook. pp. 3615-3626 (2000).
  19. Husband, D., C.T. Mant and R.S. Hodges. Development of simultaneous purification methodology for multiple synthetic peptides by reversed-phase sample displacement chromatography. J. Chromatogr. A 893, 81-94 (2000).

1995-1999

  1. Litowski, J.R., P.D. Semchuk, C.T. Mant and R.S. Hodges. Hydrophilic interaction/cation-exchange chromatography for the purification of synthetic peptides from closely related impurities: Serine side-chain acetylated peptides. J. Peptide Research 54:1-11 (1999).
  2. D'Agostino, P.A., J.R. Hancock, L.R. Provost, P.D. Semchuk and R.S. Hodges. Liquid chromatographic high resolution mass spectrometric and tandem mass spectrometric identification of synthetic peptides using electrospray ionization. J. Chromatography A 800: 89-100 (1998).
  3. Mant, C.T., L.H. Kondejewski and R.S. Hodges. Hydrophilic interaction/cation-exchange chromatography for separation of cyclic peptides. J. Chromatography A 816: 79-88 (1998).
  4. Mant, C.T., J.R. Litowski and R.S. Hodges. Hydrophilic interaction/cation exchange chromatography for separation of amphipathic a-helical peptides. J. Chromatography A 816: 65-78 (1998).
  5. Mant, C.T., H. Chao and R.S. Hodges. Effect of mobile phase on the oligomerization state of a-helical coiled-coil peptides during high-performance size-exclusion chromatography. J. Chromatography A 791: 85-98 (1997).
  6. Holm, A., S. Ostergaard, R.S. Hodges, D. Husband and S. Buus. Multiple Peptide Synthesis on Resin Immunol. Methods Manual 2: 847-851 (1997).
  7. Sereda, T.J., C.T. Mant and R.S. Hodges. Use of sodium perchlorate at low pH for peptide separations by reversed-phase chromatography. Influence of perchlorate ion on apparent hydrophilicity of positively charged side-chains. J. Chromatography A 776: 153-165 (1997).
  8. Mant, C.T., L.H. Kondejewski, P.J. Cachia, O.D. Monera and R.S. Hodges. Analysis of Synthetic Peptides by High-Performance Liquid Chromatography. In "Solid-Phase Peptide Synthesis" a volume of Methods in Enzymology, Vol. 289, pp. 426-469 (1997).
  9. Hanson, M., K.K. Unger, C.T. Mant and R.S. Hodges. Optimization strategies in ultrafast reversed-phase chromatography of proteins. Trends Anal. Chem. 15: 102-110 (1996).
  10. Godel, J.C., T.K. Basu, H.F. Pabst, R.S. Hodges, P.E. Hodges and M.L. Ng. Perinatal vitamin A (retinal) status of Northern Canadian mothers and their infants: Effects of age and race. Biol. Neonate 69: 133-139 (1996).
  11. Mant, C.T. and R.S. Hodges. Analysis of peptides by HPLC. In "High Resolution Separation of Biological Macromolecules Part B Applications" (B.L. Karger and W.S. Hancock, Eds.), a volume of Methods of Enzymology, Academic Press, San Diego, CA, 271: 3-50 (1996).
  12. Wang, J., R.S. Hodges and B.D. Sykes. Generating multiple conformations of flexible peptides in solution based on NMR nuclear overhauser effect data: Application to desmopressin. J. Am. Chem. Soc. 117: 8627-8634 (1995).
  13. Wang, J., R.S. Hodges and B.D. Sykes. Effect of trifluoroethanol on the solution structure and flexibility of desmopressin: A two-dimensional NMR study. Int. J. Peptide Protein Res. 45: 471-481 (1995).
  14. D'Agostino, P.A., J.R. Hancock, L.R. Provost, P.D. Semchuk and R.S. Hodges. High resolution electrospray mass spectrometry with a magnetic sector instrument: Accurate mass measurement and peptide sequencing. Rapid Commun. in MS 9: 597-603 (1995).
  15. Sereda, T.J., C.T. Mant and R.S. Hodges. Selectivity due to conformational differences between helical and non-helical peptides in reversed-phase chromatography. J. Chromatog. A. 695: 205-221 (1995).
  16. Wishart, D.S., C.G. Bigam, A. Holm, R.S. Hodges and B.D. Sykes. 1H, 13C and 15N random coil NMR chemical shifts of the common amino acids: I. Investigations of nearest neighbor effects. J. Biomolecular NMR Today 5: 67-81 (1995).

1990-1994

  1. Mant, C.T., T.W.L. Burke and R.S. Hodges. A rapid, simple approach to predicting effects of varying run parameters on reversed-phase gradient elution profiles of peptides. LC.GC 12:396-404 (1994).
  2. Hodges, R.S., B.-Y. Zhu, N.E. Zhou and C.T. Mant. Reversed-phase chromatography as a useful probe of hydrophobic interactions involved in protein folding and protein stability. J. Chromatography A 676: 3-15 (1994).
  3. Sereda, T.J., C.T. Mant, F.D. Sönnichsen and R.S. Hodges. Reversed-phase chromatography of synthetic amphipathic a-helical peptides as a model for ligand-receptor interactions: Effect of changing hydrophobic environment on the relative hydrophilicity/hydrophobicity of amino acid side-chains. J. Chromatography A 676: 139-153 (1994).
  4. Parker, J.M.R. and R.S. Hodges. Homology plot: Searching for homology to a family of proteins using a database of unique conserved patterns. J. Computer-Aided Molecular Design 8: 193-210 (1994).
  5. Sereda, T.J., C.T. Mant, A.M. Quinn and R.S. Hodges. Effect of the a-amino group on peptide retention behavior in reversed-phase chromatography: Determination of the pKa values of the a-amino group of 19 different N-terminal amino acid residues. J. Chromatography 646: 17-30 (1993).
  6. Hodges, R.S., T.W.L. Burke, A.J. Mendonca and C.T. Mant. Preparative reversed-phase sample displacement chromatography of peptides. In "Chromatography in Biotechnology" American Chemical Soc. Symposium Series 529, Chapter 5, pp. 59-76 (C. Horvath and L.S. Ettre, Eds.) (1993).
  7. Wang, J., F.D. Sönnichsen, R. Boyko, R.S. Hodges and B.D. Sykes. Evaluation of NMR based structure determination of flexible peptides: Application to desmopressin. In Techniques in Protein Chemistry IV,pp. 569-575 (1993). Academic Press Inc.
  8. Hanson, M., K.K. Unger, C.T. Mant and R.S. Hodges. Polymer-coated reversed-phase packings with controlled hydrophobic properties. II. Effect on selectivity of peptide separations. J. Chromatography 599: 77-85 (1992).
  9. Mant, C.T., T.W.L. Burke, A.J. Mendonca and R.S. Hodges. Preparative reversed-phase sample displacement chromatography of peptides. Proceedings of the 9th International Symposium on Preparative and Industrial Chromatography (1992) p.274-279.
  10. Hanson, M., K.K. Unger, C.T. Mant and R.S. Hodges. Polymer-coated reversed-phase packings with controlled hydrophobic properties. I. Effect on the selectivity of protein separations. J. Chromatography 599: 65-75 (1992).
  11. Zhu, B.-Y., C.T. Mant and R.S. Hodges. Mixed-mode hydrophilic and ionic interaction chromatography rivals reversed-phase chromatography for the separation of peptides. J. Chromatography 594: 75-86 (1992).
  12. Mant, C.T., N.E. Zhou and R.S. Hodges. Amino acids and peptides. In "Chromatography, 5th Edition Part B: Applications (Ed. E. Heftmann). Journal of Chromatography Library Volume 51B, Elsevier Science Publishers B.V., Amsterdam, The Netherlands, (1992) Chapter 13, pp. B75-B150.
  13. Zhu, B.-Y., C.T. Mant and R.S. Hodges. Hydrophilic-interaction chromatography of peptides on hydrophilic and strong-cation-exchange columns. Journal of Chromatogr. 548: 13-24 (1991).
  14. Hodges, R.S., T.W.L. Burke and C.T. Mant. Multi-column reversed-phase sample displacement chromatography of peptides. Journal of Chromatogr., 548: 267-280 (1991).
  15. Zhou, N.E., C.T. Mant, J.J. Kirkland and R.S. Hodges. Comparison of silica-based cyanopropyl- and octyl- reversed-phase packings for the separation of peptides and proteins. Journal of Chromatogr. 548: 179-193 (1991).
  16. Thornton, S., E. San Fabian, S. Fraga, J.M.R. Parker and R.S. Hodges. Prediction of protein secondary structures using a combined method based on the recognition, Lim and Garnier-Osguthorpe-Robson algorithms. J. Mol. Structure (Theochem)., 232: 321-336 (1991).
  17. Hodges, R.S. and C.T. Mant. Properties of peptides/proteins and practical implications. In "High Performance Liquid Chromatography of Peptides and Proteins: Separation, Analysis and Conformation" (C.T. Mant and R.S. Hodges, eds.) CRC Press, Inc., (1991) pp. 3-9.
  18. Mant, C.T. and R.S. Hodges. HPLC terminology: Practical and theoretical. In "High Performance Liquid Chromatography of Peptides and Proteins: Separation, Analysis and Conformation" (C.T. Mant and R.S. Hodges, eds.) CRC Press, Inc., (1991) pp. 69-94.
  19. Mant, C.T. and R.S. Hodges. The use of peptide standards to monitor non-ideal behavior due to underivatized silanols in reversed-phase chromatography. In "High Performance Liquid Chromatography of Peptides and Proteins: Separation, Analysis and Conformation" (C.T. Mant and R.S. Hodges, eds.) CRC Press, Inc., (1991) pp. 297-305
  20. Cachia, P.J. and R.S. Hodges. Separation of protein isoforms: An ion-exchange HPLC separation of bovine and cardiac troponin complexes. In "High Performance Liquid Chromatography of Peptides and Proteins: Separation, Analysis and Conformation" (C.T. Mant and R.S. Hodges, eds.) CRC Press, Inc., (1991) 245-253.
  21. Parker, J.M.R. and R.S. Hodges. HPLC hydrophobicity parameters: Prediction of surface and interior regions in proteins. In "High Performance Liquid Chromatography of Peptides and Proteins: Separation, Analysis and Conformation" (C.T. Mant and R.S. Hodges, eds.) CRC Press, Inc., (1991) pp. 737-749.
  22. Hodges, R.S. and C.T. Mant. Standard chromatographic conditions for size-exclusion, ion-exchange, reversed-phase, and hydrophobic interaction chromatography. In "High Performance Liquid Chromatography of Peptides and Proteins: Separation, Analysis and Conformation" (C.T. Mant and R.S. Hodges, eds.) CRC Press, Inc., (1991) pp. 11-22.
  23. Burke, T.W.L., C.T. Mant and R.S. Hodges. The effect of varying flow-rate, gradient rate and detection wavelength on peptide elution profiles in reversed-phase chromatography. In "High Performance Liquid Chromatography of Peptides and Proteins: Separation, Analysis and Conformation" (C.T. Mant and R.S. Hodges, eds.) CRC Press, Inc., (1991) pp. 307-317.
  24. Mant, C.T. and R.S. Hodges. Effects of HPLC solvents and hydrophobic matrices on denaturation of proteins. In "High Performance Liquid Chromatography of Peptides and Proteins: Separation, Analysis and Conformation" (C.T. Mant and R.S. Hodges, eds.) CRC Press, Inc., (1991) pp. 437-450.
  25. Van Eyk, J.E., C.T. Mant and R.S. Hodges. High-performance affinity chromatography of peptides and proteins. In "High Performance Liquid Chromatography of Peptides and Proteins: Separation, Analysis and Conformation" (C.T. Mant and R.S. Hodges, eds.) CRC Press, Inc., (1991) pp. 479-491.
  26. Mant, C.T. and R.S. Hodges. Mobile-phase preparation and column maintenance. In "High Performance Liquid Chromatography of Peptides and Proteins: Separation, Analysis and Conformation" (C.T. Mant and R.S. Hodges, eds.) CRC Press, Inc., (1991) pp. 37-45.
  27. Burke, T.W.L., C.T. Mant and R.S. Hodges. Comparison of peptide resolution on conventional, narrow bore and microbore reversed-phase columns. In "High Performance Liquid Chromatography of Peptides and Proteins: Separation, Analysis and Conformation" (C.T. Mant and R.S. Hodges, eds.) CRC Press, Inc., (1991) pp. 679-686.
  28. Mant, C.T. and R.S. Hodges. Development and application of a computer simulation program for method development and teaching in HPLC: ProDigest-LC. In "High Performance Liquid Chromatography of Peptides and Proteins: Separation, Analysis and Conformation" (C.T. Mant and R.S. Hodges, eds.) CRC Press, Inc., (1991) pp. 705-723.
  29. Hodges, R.S., Burke, T.W.L., C.T. Mant and S.-M. Ngai. Preparative reversed-phase gradient elution chromatography on analytical columns. In "High Performance Liquid Chromatography of Peptides and Proteins: Separation, Analysis and Conformation" (C.T. Mant and R.S. Hodges, eds.) CRC Press, Inc., (1991) pp. 773-782.
  30. Lee, K.K., J.A. Black and R.S. Hodges. Separation of intrachain disulfide bridged peptides from their reduced forms by reversed-phase chromatography. In "High Performance Liquid Chromatography of Peptides and Proteins: Separation, Analysis and Conformation" (C.T. Mant and R.S. Hodges, eds.) CRC Press, Inc., (1991) pp. 389-398.
  31. Mant, C.T. and R.S. Hodges. Requirement for peptide standards to monitor ideal and non-ideal behavior in size-exclusion chromatography. In "High Performance Liquid Chromatography of Peptides and Proteins: Separation, Analysis and Conformation" (C.T. Mant and R.S. Hodges, eds.) CRC Press, Inc., (1991)pp. 125-134 .
  32. Mant, C.T. and R.S. Hodges. The use of peptide standards for monitoring ideal and non-ideal behavior in cation-exchange chromatography. In "High Performance Liquid Chromatography of Peptides and Proteins: Separation, Analysis and Conformation" (C.T. Mant and R.S. Hodges, eds.) CRC Press, Inc., (1991) pp. 171-185.
  33. Mant, C.T. and R.S. Hodges. Requirement for peptide standards to monitor column performance and the effect of column dimensions, organic modifiers and temperature in reversed-phase chromatography. In "High Performance Liquid Chromatography of Peptides and Proteins: Separation, Analysis and Conformation" (C.T. Mant and R.S. Hodges, eds.) CRC Press, Inc., (1991) pp. 289-295.
  34. Mant, C.T. and R.S. Hodges. The effects of anionic ion-pairing reagents on peptide retention in reversed-phase chromatography. In "High Performance Liquid Chromatography of Peptides and Proteins: Separation, Analysis and Conformation" (C.T. Mant and R.S. Hodges, eds.) CRC Press, Inc., (1991) pp. 327-341.
  35. Mant, C.T. and R.S. Hodges. On-line derivatization of silica supports for regeneration of reversed-phase columns. In "High Performance Liquid Chromatography of Peptides and Proteins: Separation, Analysis and Conformation" (C.T. Mant and R.S. Hodges, eds.) CRC Press, Inc., (1991) pp. 57-65.
  36. Black, J.A. and R.S. Hodges. Peak Identification in HPLC of peptides using spectral analysis and second order spectroscopy of aromatic amino acid residues. In "High Performance Liquid Chromatography of Peptides and Proteins: Separation, Analysis and Conformation" (C.T. Mant and R.S. Hodges, eds.) CRC Press, Inc., (1991) pp. 563-570.
  37. Zhou, N.E., P.D. Semchuk, C.M. Kay and R.S. Hodges. The effect of hydrophobic residue distribution in a-helical peptides on peptide retention behavior in reversed-phase HPLC. In "High Performance Liquid Chromatography of Peptides and Proteins: Separation, Analysis and Conformation" (C.T. Mant and R.S. Hodges, eds.) CRC Press, Inc., (1991) pp. 643-658.
  38. Burke, T.W.L., J.A. Black, C.T. Mant and R.S. Hodges. Preparative reversed-phase shallow gradient approach to the purification of closely related peptide analogs on analytical instrumentation. In "High Performance Liquid Chromatography of Peptides and Proteins: Separation, Analysis and Conformation" (C.T. Mant and R.S. Hodges, eds.) CRC Press, Inc., (1991) pp. 783-791.
  39. Mant, C.T. and R.S. Hodges. Preparative reversed-phase sample displacement chromatography of synthetic peptides. In "High Performance Liquid Chromatography of Peptides and Proteins: Separation, Analysis and Conformation" (C.T. Mant and R.S. Hodges, eds.) CRC Press, Inc., (1991) pp. 793-807.
  40. Mant, C.T., T.W.L. Burke, N.E. Zhou, J.M.R. Parker and R.S. Hodges. Reversed-phase chromatographic method development for peptide separations using a computer simulation program: ProDigest LC. In "Computer-Assisted Method Development for High-Performance Liquid Chromatography" (J.L. Glajch and L.R. Snyder, Eds.) Elsevier (1990) pp. 365-382. (reprinted from J. Chromatogr. 485 (1989) 365-382).
  41. Thornton, S., E. San Fabian, S. Fraga, J.M.R. Parker and R.S. Hodges. Theoretical prediction of secondary structures of proteins using recognition factors. Journal of Molecular Structure 226: 87-97 (1990).
  42. Mant, C.T. and R.S. Hodges. Optimization and prediction of peptide retention behavior in reversed-phase chromatography. In "HPLC of proteins, peptides and polynucleotides: Contemporary topics and applications" (M.T.W. Hearn, editor). VCH Publishers, Inc., U.S.A., 277-305 (1991).
  43. Zhou, N.E., C.T. Mant and R.S. Hodges. Effect of preferred binding domains on peptide retention behavior in reversed-phase chromatography: Amphipathic a-Helices. Peptide Research 3: 8-20 (1990). Reprinted in Biochromatography 5: 127-139 (1990).
  44. Mant, C.T. and R.S. Hodges. HPLC of Peptides. In "HPLC of biological macromolecules: Methods and applications" (K. Gooding and F. Regnier, Eds.) Marcel Dekker, Inc., New York, N.Y. (1990) pp. 301-332.

1985-1989

  1. Mant, C. T., T.W.L. Burke, N.E. Zhou, J.M.R. Parker and R.S. Hodges. Reversed-phase chromatographic method development for peptide separations using a computer simulation program: ProDigest-LC. J. Chromatogr. 485: 365-382 (1989).
  2. Mant, C.T. and R.S. Hodges. Optimization of peptide separations in high performance liquid chromatography. J. Liquid Chromatogr. 12: 139-172 (1989).
  3. Mant, C.T., N.E. Zhou and R.S. Hodges. Correlation of protein retention times in reversed-phase chromatography with polypeptide chain length and hydrophobicity. J. Chromatography 476:363-375 (1989).
  4. Burke, T.W.L., C.T. Mant, J.A. Black and R.S. Hodges. Strong cation-exchange HPLC of peptides: Effect of non-specific hydrophobic interactions and linearization of peptide retention behavior. J. Chromatography 476: 377-389 (1989).
  5. Hodges, R.S., J.M.R. Parker, C.T. Mant and R.R. Sharma. Computer simulation of HPLC separations of peptide and protein digests for development of size-exclusion, ion-exchange and reversed-phase chromatographic methods. J. Chromatogr. 458: 147-167 (1988).
  6. Mant, C.T., T.W.L. Burke, J.A. Black and R.S. Hodges. The effect of peptide chain length on peptide retention behavior in reversed-phase chromatography. J. Chromatogr. 458: 193-205 (1988).
  7. Fraga, S., R.S. Hodges, J.M.R. Parker, E. San-Fabian and A.V. Bunge. Computer modelling of protein structures. J. Molecular Structure (Theochem) 166: 129-134 (1988).
  8. Burke, T.W.L., C.T. Mant and R.S. Hodges. A novel approach to reversed-phase preparative high-performance liquid chromatography of peptides. J. Liquid Chromatography 11: 1229-1247 (1988).
  9. Hearn, M.T.W., M.I. Aguilar, C.T. Mant and R.S. Hodges. Evaluation of the use of hydrophobicity coefficients for the prediction of peptide elution profiles. J. Chromatogr. 438: 197-210 (1988).
  10. Hodges, R.S., T.W.L. Burke and C.T. Mant. Preparative purification of peptides by reversed-phase chromatography: Sample displacement mode versus gradient elution mode. J. Chromatogr. 444: 349-362 (1988).
  11. Parker, J.M.R., C.T. Mant and R.S. Hodges. A practical approach to the preparative purification of peptides using analytical instrumentation with analytical and semipreparative columns. Chromatographia 24: 832-838 (1987).
  12. Mant, C.T., T.W.L. Burke and R.S. Hodges. Optimization of peptide separations in reversed-phase HPLC: Isocratic versus gradient elution. Chromatographia 24: 565-572 (1987).
  13. Mant, C.T. and R.S. Hodges. Monitoring free silanols on reversed-phase supports with peptide standards. Chromatographia 24: 805-814 (1987).
  14. Mant, C.T. and R.S. Hodges. On-line derivatization of silica columns for regeneration and preparation of reversed-phase supports. J. Chromatogr. 409: 155-173 (1987).
  15. Guo, D., C.T. Mant and R.S. Hodges. Effects of ion-pairing reagents on the prediction of peptide retention in reversed-phase high-performance liquid chromatography. J. Chromatogr. 386: 205-222 (1987).
  16. Mant, C.T., J.M.R. Parker and R.S. Hodges. Size-exclusion HPLC of peptides: Requirement for peptide standards to monitor non-ideal behavior. J. Chromatogr. 397: 99-112 (1987).
  17. Mant, C.T., J.M.R. Parker and R.S. Hodges. On-line derivatization to restore reversed-phase column performance in peptide separations. LC.GC Magazine of Liquid and Gas Chromatography 4: 1004-1009 (1986).
  18. Mant, C.T. and R.S. Hodges. Requirement for peptide standards in reversed-phase high performance liquid chromatography. LC Magazine, Liquid Chromatography and HPLC 4: 250-254 (1986).
  19. Parker, J.M.R., D. Guo and R.S. Hodges. A new hydrophilicity scale derived from HPLC peptide retention data: Correlation of predicted surface residues with antigenicity and X-ray derived accessible sites. Biochemistry 25: 5425-5432 (1986).
  20. Guo, D., C.T. Mant, A.K. Taneja, J.M.R. Parker and R.S. Hodges. Prediction of peptide retention times in reversed-phase high-performance liquid chromatography. I. Determination of retention coefficients of amino acid residues using model synthetic peptides. J. Chromatogr. 359: 499-518 (1986).
  21. Guo, D., C.T. Mant, A.K. Taneja and R.S. Hodges. Prediction of peptide retention times in reversed-phase high-performance liquid chromatography. II. Correlation of observed and predicted peptide retention times and factors influencing retention times of peptides. J. Chromatogr. 359: 519-532 (1986).
  22. Mant, C.T. and R.S. Hodges. A general method for the separation of cyanogen bromide digests of proteins by high performance liquid chromatography: Rabbit skeletal troponin I. J.Chromatogr. 326: 349-356 (1985).
  23. Ingraham, R.H., S.Y.M. Lau, A.K. Taneja and R.S. Hodges. Denaturation and the effects of temperature on hydrophobic interaction and reversed-phase high performance liquid chromatography of proteins: Bio-Gel TSK-Phenyl-5-PW support. J. Chromatogr. 327: 77-92 (1985).
  24. Mant, C.T. and R.S. Hodges. Separation of peptides by strong cation-exchange high performance liquid chromatography. J. Chromatogr. 327: 147-155 (1985).
  25. Cachia, P.J., J. Van Eyk, W.D. McCubbin, C.M. Kay and R.S. Hodges. High performance ion-exchange liquid chromatographic purification of bovine cardiac and rabbit skeletal muscle troponin subunits. J. Chromatogr. 343: 315-328 (1985).

1980-1984

  1. Taneja, A.K., S.Y.M. Lau and R.S. Hodges. Separation of hydrophobic peptide polymers by size-exclusion and reversed-phase high performance liquid chromatography. J. Chromatogr. 317: 1-10 (1984).
  2. Lau, S.Y.M., A.K. Taneja and R.S. Hodges. Effects of HPLC solvents and hydrophobic supports on the secondary and quaternary structure of a model protein: Reversed-phase and size-exclusion high performance liquid chromatography. J. Chromatogr. 317: 129-140 (1984).
  3. Cachia, P.J., J. Van Eyk, P.C.S. Chong, A. Taneja and R.S. Hodges. Separation of basic peptides by cation-exchange high pressure liquid chromatography. J. Chrom. 266: 651-659 (1983).
  4. Hofmann, T. and R.S. Hodges. A new chromophoric substrate for penicillopepsin and other fungal aspartyl proteinases. Biochem. J. 203: 603-610 (1982).
  5. Chong, P.C.S. and R.S. Hodges. A new heterobifunctional crosslinking reagent for the study of biological interactions between proteins. I. Design, synthesis and characterization. J. Biol. Chem. 256: 5064-5070 (1981).
  6. Chong, P.C.S. and R.S. Hodges. A new heterobifunctional crosslinking reagent for the study of biological interactions between proteins. II. Application to the troponin C-troponin I interaction. J. Biol. Chem. 256: 5071-5076 (1981).

1975-1979

  1. Tjoeng, F.-S., E.K. Tong and R.S. Hodges. Liquid-phase synthesis of protected peptides on the new 3-nitro-4-bromomethylbenzoyl polyethylene glycol support. J. Org. Chem. 43: 4190-4194 (1978).
  2. Tjoeng, F.-S. and R.S. Hodges. Cleavage of protected amino acids and peptides from the new o-nitrobenzoyl polyethylene glycol support by catalytic hydrogenolysis. Tetrahedron Letters 15: 1273-1276 (1979).
  3. Mitchell, A.R., B.W. Erickson, M.N. Ryabtsev, R.S. Hodges and R.B. Merrifield. Boc-amino-acyl-4-(oxymethyl)phenyl-acetamidomethyl-resin, a more acid-resistant support for solid-phase peptide synthesis. J. Am. Chem. Soc. 98: 7357-7362 (1976).
  4. Tjoeng, F.-S., W. Staines, S.A. St.-Pierre and R.S. Hodges. Liquid-phase method for peptide synthesis utilizing photolytic cleavage from a new o-nitrobenzoyl polyethylene glycol support. Biochim. Biophys. Acta (Protein Structure) 490: 489-496 (1977).
  5. Hodges, R.S. and R.B. Merrifield. Monitoring of solid-phase peptide synthesis by an auto-mated spectrophotometric picrate method. Anal. Biochem. 65: 241-272 (1975).

1970-1974

  1. Hodges, R.S. and R.B. Merrifield. Synthesis of O-methyl-L-serine and Na-tert-butyloxy-carbonyl-0-methyl-L-serine. J. Org. Chem. 39: 1870-1872 (1974).

Proceedings Publications

2015-2019

N/A

2010-2014

  1. Cepeniene, D., R. Irvin, R.S. Hodges and L. Gera. Selective labeling of lysine-containing biologically active peptides at the N-terminus with alexa fluor dye. In Peptides: Building Bridges (M. Lebl, Ed.), Proceedings of the 22nd American Peptide Symposium, San Diego, CA, pp. 76-77 (2011).

2005-2009

  1. Kovacs, J.M., C.T. Mant and R.S. Hodges. Determination of Intrinsic Hydrophilicity /Hydrophobicity Coefficients of Amino Acid Side-Chains using Synthetic Model Peptides. In Understanding Biology Using Peptides: Proceedings of the 19th American Peptide Symposium. San Diego, CA (2005) (S.E. Blondelle, Ed.) Springer Science, New York, NY pp 679-682 (2006).

2000-2004

  1. Mehok, A.R., C.T. Mant, B. Tripet and R.S. Hodges. Isocratic reversed-phase chromatography separation and purification of peptides. In Peptide Revolution: Genomics, Proteomics and Therapeutics, Proceedings of the Eighteenth American Peptide Symposium Boston, MA (2003) (M. Chorev and T. K. Sawyer, Eds.) American Peptide Society pp. 145-146 (2004).
  2. Popa, T.V., C.T. Mant and R.S. Hodges. Comparison of effect of anionic ion-pairing reagents on capillary electrophoresis and reversed-phase chromatography of cationic random coil peptides. In Peptide Revolution: Genomics, Proteomics and Therapeutics, Proceedings of the Eighteenth American Peptide Symposium Boston, MA (2003) (M. Chorev and T. K. Sawyer, Eds.) American Peptide Society pp. 123-124 (2004).
  3. Lee, D.L., C.T. Mant and R.S. Hodges. Monitoring self-association of small amphipathic molecules by temperature profiling in reversed-phase chromatography. In Peptide Revolution: Genomics, Proteomics and Therapeutics, Proceedings of the Eighteenth American Peptide Symposium Boston, MA (2003) (M. Chorev and T. K. Sawyer, Eds.) American Peptide Society pp. 149-150 (2004).
  4. Chen, Y. and R.S. Hodges. Effect of secondary structure on selectivity of reversed-phase chromatography for peptide separations at varying temperatures. In Peptide Revolution: Genomics, Proteomics and Therapeutics, Proceedings of the Eighteenth American Peptide Symposium Boston, MA (2003) (M. Chorev and T. K. Sawyer, Eds.) American Peptide Society pp. 151-152) (2004).
  5. Merrifield, B., G. Barany, C.M. Deber, M. Goodman, R.S. Hodges, V.J. Hruby, T.W. Muir, R. Offord, A.F. Spatola, D.F. Veber and G.B. Fields. Perspectives for the new peptide millennium. InPeptides for the New Millennium”, Proceedings of the Sixteenth American Peptide Symposium (G.B. Fields, J.P. Tam and G. Barany, Eds.), Kluwer Academic Publishers, pp. 797-804 (2000).

1995-1999

  1. Mant, C.T. and R.S. Hodges. Reversed-phase chromatography as a mimic of ligand-receptor interactions. In: Peptides: Chemistry, Structure and Biology, Proceedings of the Fourteenth American Peptide Symposium (P. Kaumaya and R.S. Hodges, Eds.) Mayflower Scientific Ltd., England, pp. 46-47 (1996).
  2. Semchuk, P.D., O.D. Monera, L.H. Kondejewski, C. Gannon, L. Daniels, I. Wilson and R.S. Hodges. Preferential disulfide-bridge formation for hetero-dimers by derivatization with 2,2'-dithiodipyridine. In: Peptides: Chemistry, Structure and Biology, Proceedings of the Fourteenth American Peptide Symposium (P. Kaumaya and R.S. Hodges, Eds.) Mayflower Scientific Ltd., England, pp. 73-74 (1996).

1990-1994

  1. Wang, J., R.S. Hodges and B.D. Sykes. Elucidation of multiple conformations for flexible peptides from average NOE data: Application to desmopressin. In: Peptides: Chemistry, Structure and Biology, Proceedings of the Thirteenth American Peptide Symposium (R.S. Hodges and J.A. Smith, Eds.), Escom Science Publishers, Leiden, The Netherlands, pp.914-917 (1994).
  2. Zhu, B.-Y., C.T. Mant and R.S. Hodges. Mixed-mode hydrophilic and ionic interaction chromatography rivals reversed-phase chromatography for the separation of peptides. In "Peptides: Chemistry and Biology", Proceedings of the 12th American Peptide Symposium (J. Smith and J.E. Rivier, Eds.) Escom Science Publishers, The Netherlands (1992) 546-548.
  3. Lee, K.K., J.A. Black and R.S. Hodges. Air oxidation of peptides and separation of their reduced and intrachain disulfide bridged forms by RPHPLC. In "Peptides: Chemistry, Structure and Biology". Proceedings of the Eleventh American Peptide Symposium (J.E. Rivier and G.R. Marshall, Eds.), Escom Science Publishers, The Netherlands (1990) pp. 999-1002.

​ 1985-1989

  1. Hodges, R.S., T.W.L. Burke and C.T. Mant. A novel approach to the purification of peptides by reversed-phase HPLC: Sample displacement chromatography. In "Peptides: Chemistry and Biology - Proceedings of the Tenth American Peptide Symposium (G. R. Marshall, Editor) Escom Science Publishers, Leiden, The Netherlands, pp. 226-228 (1988).
  2. Guo, D., C.T. Mant, J.M.R. Parker and R.S. Hodges. Hydrophilicity parameters in peptides.1. Prediction of peptide retention in reversed-phase HPLC (RPC). 2. Prediction of hydrophilic regions on the surface of proteins. In "Peptides: Structure and Function-Proceedings of the Ninth American Peptide Symposium" (C.M. Deber, V.J. Hruby and K.D. Kopple, eds.) Pierce Chemical Co. pp. 23-26 (1985).​

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