The development of new separation methodology is critical to research in academia, biotechnology and pharmaceutical sectors. A few examples of new methodology developed in our laboratory are listed below: a new mixed-mode hydrophilic/cation-exchange chromatographic method for the separation of peptides that rivals reversed-phase chromatography (RPC) where selectivity is completely different; a new capillary electrophoresis method called “Ion-interaction capillary zone electrophoresis” which separates not only by charge/ mass ratio but by subtle differences in hydrophobicity as well; a novel method to measure self-association of small amphipathic molecules referred to as temperature profiling in RPC; a one-step purification of recombinant proteins from a whole cell extract by RPC; and we have determined the intrinsic hydrophilicity/hydrophobicity of amino acid side-chains in peptides in the absence of nearest-neighbor or conformational affects and showed that this scale was the most accurate determination of side-chain hydrophilicity/hydrophobicity and of general value to all fields of biomedical science.
Selected publications are listed.
Mant, C.T. and R.S. Hodges. Separation of peptides on HALO 2-micron particles. Current Protocols in Protein Science 85: 11.6.1-11.6.16 (2016). doi:10.1002/cpps.12 (Wileyonlinelibrary.com, John Wiley & Sons, Inc.).
Mant, C.T., Z. Jiang, B. E. Boyes and R.S. Hodges. An improved approach to hydrophilic interaction chromatography of peptides: Salt gradients in the presence of high isocratic acetonitrile concentrations. J. Chromatography A 1277: 15-25 (2013). PMC3639484 PMID23332786
Mant, C.T. and R.S. Hodges. Design of peptide standards with the same composition and minimal sequence variation to monitor performance/selectivity of reversed-phase matrices. J. Chromatography A , 1230: 30-40 (2012). PMC3294100 PMID22326185
Mant, C.T., D. Cepeniene and R.S. Hodges. Reversed-phase HPLC of Peptides: Assessing column and solvent selectivity on standard, polar embedded and polar end-capped columns. J. Separation Science 33:3005-3021 (2010). No PMC PMID21038458
Mant, C.T., J.M. Kovacs, H.M. Kim, D.D. Pollock and R.S. Hodges. Intrinsic amino acid side-chain hydrophilicity/hydrophobicity coefficients determined by reversed-phase high-performance liquid chromatography of model peptides: comparison with other hydrophilicity/hydrophobicity scales. Biopolymers (Peptide Science) 92: 573-595 (2009). PMC2792893 PMID19795449