HTS/HCS Discovery

HTS/HCS Drug Discovery and Hit-to-Lead Drug Development

This site is specific for researchers interested in utilizing the D3SR Arm, which specializes in HTS/HCS probe and drug discovery using cellular and biochemical systems. We also specialize in three-dimensional (3D) HTS/HCS of complex cell-based models, organoids and tumor organoids (cell line or patient derived). This facility is newly renovated and features a new state-of-the-art custom robotic automation platform (the Explorer G3) that integrates new HTS/HCS instrumentation and equipment from PerkinElmer, Tek-Matic, and other leading vendors.

The Explorer G3 Work Station (PerkinElmer) is a custom designed automation platform that is fully enclosed, maintaining a clean sterile environment through a HEPA filtration system. The Explorer G3 has three levels, the main deck, and a middle and lower deck used to house instrumentation and equipment. The Explorer G3 has a track system robotic arm that integrates equipment on both the main and middle decks.

  • Janus G3 Automated Liquid Handling platform (96-and 384-channel MDT heads and pintool) 
  • Opera Phenix Plus HCS system (4 sCMOS cameras with live cell chamber)
  • Envision multi-mode plate reader (hybrid system of filters and quad monochromators) The
  • BioSpot (custom liquid handling platform for precise droplet delivery)
  • Liconic STX220 Cell Culture Incubator
  • Liconic STX500 Drug Library Storage Freezer.  •Liconic LPX220 Plate Hotel 
  • ELx405 Plate Washer (BioTek)
  • Centrifuge with Loader (Agilent)
  • 4titude 4AS Automated heat sealer/peeler (Books)
  • MultiFlo Bulk Liquid Dispenser (BioTek)

Other Equipment Utilized for Assay Development Not Integrated with The Explorer G3

  • Generation 1 Opera Phenix HCS system
  • Janus Automated Liquid Handling platform (96- channel MDT heads and 8-channel Varispan)
  • Envision multi-mode plate reader (Filter based)
  • Columbus server
  • 4-foot bio-safety cabinet (safety level II)

Compound Libraries 

Kinase Inhibitor Library (SelleckChem): 875 compounds including clinically relevant or FDA approved kinase inhibitors.

Spectrum Collection (Discovery Systems): 2,320 compounds approved drugs (50%), diverse natural products (30%), and other bioactive compounds (20%).

Custom Drug-Like Diversity Sets (120,000 compounds): These libraries are intended for discovering drug-like molecules that can be translated into novel intellectual property. Inherent to these libraries are derivatives of each chemical scaffold, which can yield SAR (structure-activity relationship) that can guide downstream medicinal chemistry efforts. These libraries have been filtered to remove undesirable compounds (PAINS)

  • Diversity Library 1 has 20,000 compounds (Life Chemicals).
  • Diversity Library 2 has 50,000 compounds (ChemBridge)
  • Diversity Library 3 has 50,000 CNS specific compounds (ChemBridge)

For more information on equipment, instrumentation and pricing, or to obtain consultation or project cost estimates please click here to visit the D3SR Arm 1 iLabs Website.

Contact Us

Dan LaBarbera, PhD

Dan LaBarbera, PhD
Associate Professor
D3SR Arm 1-Director

Qiong Zhou

Qiong Zhou, MS
Senior Professional Research Assistant
D3SR Arm 1-Laboratory Manager


Hours of Operation

Monday-Friday, 9 am -5 pm        

p | (303)724-4116


12850 E Montview Blvd, V20-2211
Skaggs School of Pharmacy and Pharmaceutical Sciences
University of Colorado Anschutz Medical Campus
Aurora, CO 80045

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