Patricia Ernst, PhD

Professor


 
Patricia Ernst

Contact Information:

University of Colorado Denver
Anschutz Medical Campus
12800 E. 19th Ave
Research Complex 1 
Aurora, CO  80045

Phone: (303) 724-8804
E-mail: patricia.ernst@cuanschutz.edu
Office: RC1-North, P18-4117

Ernst Lab​

Global and unique functions of H3K4 methyltransferases in hematopoiesis and leukemia

The mechanisms that control the balance between self-renewing and differentiating hematopoietic stem cell (HSC) divisions are one major interest of our group. Since these processes are often deregulated in leukemia, we study molecular pathways in both normal and leukemic cells to better understand how proliferation, self-renewal and differentiation are disrupted by leukemia-associated fusion oncogenes.

Our lab initially focused on determining functions of the Mixed Lineage Leukemia (MLL/MLL1/KMT2A) gene in normal hematopoiesis due to its discovery as a leukemia proto-oncogene. The human MLL1 gene, located on human chromosome 11q23, is altered by chromosomal translocation frequently in infant acute lymphocytic leukemia (ALL) and therapy-induced acute leukemia, as well as approximately 10% of adult myeloid leukemia. Chromosomal translocations disrupt the MLL1 gene, generally producing chimeric fusion oncoproteins with altered properties relative to the wild-type MLL1. The poor response of patients with MLL translocations to existing therapy motivates us to more deeply understand the role of MLL1 in hematopoiesis and leukemia.

Our research projects extend from the following areas:

Unique roles for the H3K4-methyltransferase MLL1 in hematpoietic development. Using diverse loss- and gain-of-function murine models, we have established that Mll1 is essential for the development of HSCs in the aorta-gonad-mesonephros (AGM) region (Ernst et al. Developmental Cell 2004). Using pluripotent cells, we have shown that MLL1 levels are critical for the emergence of multipotent hematopoietic progenitors from hemogenic endothelium (Ernst et al. Current Biology 2004, Yang et al. Stem Cell Reports 2020). Unique vascular defects observed in germline Mll1 knockout embryos also may contribute to early hematopoietic phenotypes. Hematopoietic-targeted Mll1 deletion illustrated that fetal liver HSCs develop and expand throughout gestation, but fail to acquire functional properties of adult HSCs (Gan et al. Leukmemia 2010). Using these model systems, we are defining MLL1-dependent transcriptional networks, how they support hematopoietic development, how they differ from leukemogenic programs, and whether they can be exploited to alter stem cell functions.

Mll1 function in adult hematopoiesis and mechanisms of gene regulation. Conditional knockout approaches identified HSCs and pre-B cells as cell types in which Mll1 plays an important homeostasis function (Jude et al. Cell Stem Cell 2007, Gan et al. Journal of Immunology 2018). We have employed ChIP-seq/PCR and transcriptome analyses to identify direct target genes of MLL1 and have also determined the role of Menin as a partner in regulating target genes. Our work showed that Menin and MLL1 regulate largely distinct gene sets (Li et al. Blood 2013, Chen et al. Experimental Hematology 2018). Mechanistic studies illustrate that the MLL1 protein complex likely regulates important HSC genes through its association with a histone acetyltransferases, rather than through its own histone methyltransferase activity (Mishra et al. Cell Reports 2014).

Histone methyltransferases as drug targets in leukemia. Although MLL1 does not play a role in initiating or sustaining MLL-fusion oncoprotein initiated leukemia (Chen et al. Cancer Cell 2017, Chen et al. Cell Cycle 2018) its loss can rate-limit MN-1 or Nup98-Hoxa9 initiated AML (Reidel et al. Journal of Clinical Investigation 2016; Xu et al. Cancer Cell 2016), or BCR-ABL initiated B-ALL (Gan et al. Journal of Immunology 2018). These observations suggest that therapeutic targeting of MLL1 may have value in particular settings.  In contrast, we found that the closely related MLL2 (KMT2B) controls several key survival pathways in AML that have nothing to do with MLL-fusion oncoprotein deregulated pathways, making it an attractive drug target in AML generally (Chen et al. Cancer Cell 2017, Chen et al. Experimental Hematology 2018). In addition to understanding specific versus general roles of H3K4me3 modification by particular enzymes, we are interested in the impact of these enzymes/modifications on transcriptional plasticity and evasion from therapy.

Current Lab Members

First NameLast NameMiddle InitialDegreePosition
AmyHoang  Undergraduate
RitsukoIwanaga PhDInstructor
MatthiasLeon BSResearch Assistant
WenjuanLiao PhDPostdoctoral Fellow
JosephSoukup  Undergraduate
IraTandon  Undergraduate
Weiwei Yang BSGraduate Student

 

Academic Alumni

First NameMiddle InitialLast NameDegreePosition
ErikaArtingerJD, PhDSenior Staff Attorney, University of Oklahoma, OK
YufeiChenPhDStaff Scientist at Seattle Genetics, WA
KellyFaulkMDExperimental Theraputics, Children’s Hospital Colorado, CO
TaoGanPhDAssociate Professor, Gannan Medical University, PRC
Craig D.JudePhDVisiting Assistant Professor, Bard College, NY
YJKimMDWriter and Medical Resident at Stanford University, CA
Jasmine (Yoo)LeeBSGraduate Student at Emory, GA
BinE.LiPhDPostdoctoral Fellow, Children’s Hospital Boston, MA
BibhuMishraPhDSenior Scientist, Vor Pharma, Boston, MA
FlorenceRabianMD, MSPhysician, Blood Disease Unit, St. Louis Hospital, Paris, France
TatsuroWatanabePhDAssociate Professor Saga University, Japan
DiyongXuMS, MBAPrincipal, Orbimed Healthcare Fund Management
WeiweiYangPhDPostdoctoral Fellow, Research Division, New England Biolabs, MA

  1. Yang W, Trahan GD, Howell ED, Speck NA, Jones KL, Gillen AE, Riemondy K, Hesselberth J, Bryder D and P Ernst. Enhancing hematopoiesis from murine embryonic stem cells through MLL1-induced activation of a Rac/Rho/integrin signaling axis. Stem Cell Reports, 2020 in press.
  2. Addicks GC, Brun CE, Sincennes M-C, Saber J, Porter CJ, Stewart AF, Ernst P and MA Rudnicki. MLL1 is required for PAX7 expression and satellite cell self-renewal in mice. Nature Communications, 2019. Sep 18;10(1):4256
  3. Hurtz C, Chan LN, Geng H, Ballabio E, Xiao G, Khoury H, Chen CW, Armstrong SA, Chen J, Ernst P, Melnick AM, Milne TZ and M Muschen. Rationale for targeting BCL6 in MLL-rearranged acute lymphoblastic leukemia. Genes Dev, 2019 Sep 1;33(17-18):1265-1279.
  4. Chen Y and P Ernst. Hematopoietic transformation in the absence of MLL1/KMT2A: distinctions in target gene reactivation. Cell cycle, 2019 Jun 4:1-7 
  5. Chen Y, Jones KL , Anastassiadis K, Kranz A, Stewart AF, Arndt K, Grembecka J, Meyerson M and P Ernst. Distinct Pathways Affected by Menin versus MLL1/MLL2 in MLL-rearranged Acute Myeloid Leukemia. Experimental Hematology, 2019 Jan;69:37-42.
  6. Gan T, Li BE, Mishra BP, Jones, KL and P Ernst.  Lymphocyte-specific loss of MLL reveals preBCR checkpoint defects. Journal of Immunology, 2018 Mar 1;200(5):1682-1691
  7. Chen Y, Anastassiadis K, Kranz A, Stewart AF, Arndt K, Waskow C, Yokoyama A, Jones KL, Neff T, Lee Y and P Ernst. MLL2, not MLL1, plays a major role in sustaining MLL-rearranged Acute Myeloid Leukemia. Cancer Cell, 2017 Jun 12;31(6):755-760
  8. Xu H, Valerio DG, Eisold ME, Sinha A, Koche RP, Hu W, Chen CW, Chu SH, Brien GL, Hsieh JJ, Ernst P and SA Armstrong. NUP98-Fusion proteins interact with the NSL and MLL1 complexes to drive leukemogenesis. Cancer Cell, 2016 Dec 12;30(6):863-878
  9. Lin W, Francis JM, Li H, Gao X, Pedamallu CS, Ernst P and Meyerson M. Kmt2a cooperates with menin to suppress tumorigenesis in mouse pancreatic islets. Cancer Biol Ther. 2016 Dec;17(12):1274-1281
  10. Shen EY, Jiang Y, Javidfar B, Kassim B, Loh YH, Ma Q, Mitchell AC, Pothula V, Stewart AF, Ernst P, Yao WD, Martin G, Shen L, Jakovcevski M, Akbarian S. Neuronal Deletion of Kmt2a/Mll1 Histone Methyltransferase in Ventral Striatum is Associated with Defective Spike-Timing Dependent Striatal Synaptic Plasticity, Altered Response to Dopaminergic Drugs and Increased Anxiety. Neuropsychopharmacology. 2016 Dec;41(13):3103-3113.
  11. Riedel SS, Haladyna JN, Bezzant M, Stevens B, Pollyea DA, Sinha AU, Armstrong SA, Wei Q, Pollock RM, Daigle SR, Jordan CT, Ernst P, Neff T, Bernt KM. MLL1 and DOT1L cooperate with meningioma-1 to induced acute myeloid leukemia. Journal of Clinical Investigation, 2016 126(4):1438-50.
  12. Jakovcevski M, Ruan H, Shen EY, Dincer A, Ma Q, Peter C, Jiang Y, Cheung I, Mitchell AC, Ernst P, Yao W-D, Akbarian S, Stewart F, Pothula V and B. Javidfar. "Neuronal Kmt2a/Mll1 Histone Methyltransferase is essential for Prefrontal Synaptic Plasticity and Working Memory" Journal of Neuroscience, 2015 Apr 1;35(13):5097-6108
  13. R Bharadwaj, C J Peter, Y Jiang, P Roussos, A Vogel-Cierna, E Shen, A Mitchell, W Mao, C Whittle, A Dincer, M Jakovcevski, V Pothula, T Rasmussen, S G Giakoumaki, P Bitsios, P D Gardner, P Ernst, S Ghose, P Sklar, V Haroutunian, C Tamminga, R H Myers, K Futai, M A Wood and S Akbarian, Conserved higher order chromatin regulates NMDA receptor gene expression and cognition. Neuron, 2014 Dec 3;84(5):997-1008
  14. Mishra BP, Zaffuto KM, Artinger EL, Org T, Mikkola HKA, Cheng C, Djabali M and P Ernst. The histone methyltransferase activity of MLL1 is dispensable for hematopoiesis and leukemogenesis. Cell Reports, 2014 May 22;7(4):1239-47