Electron Microscopy Core Facility


The Electron Microscopy Core Facility at the University of Colorado School of Medicine is a multi-user/service facility that provides biological-biomedical researchers with instrumentation and image analysis on a recharge basis. We welcome investigators from all academic institutions and private sectors.

We offer comprehensive specimen preparation and imaging services, including negative staining, plastic embedding, ultrathin sectioning, and immunogold labeling for electron microscopy studies. For more information or if you need assistance with anything not included here, simply email the Core Director, Anza Darehshouri, at [email protected].


Our Team

Anza Darehshouri, PhD

Director:

Anza Darehshouri, PhD

Research Assistant Professor

Electron Microscopists:

Connon Thomas, PhD
Senior Research Associate

Email Contact: [email protected]

Mei Li image

 

Mei Li
Professional Research Assistant 

Email Contact: [email protected]


Equipment/Resources

  • JEOL JEM-120i is a transmission electron microscope (TEM) with a maximum accelerating voltage of 120kV, equipped with a low-mount 15-megapixel NanoSprint15LMarkII AMT camera. 
  • Leica Ultracut EM UC7 equipped with a Leica EM FC7 Cryo Chamber
  • Leica UC Enuity - Advanced Instrument M80
  • Pelco Glow Discharge Unit
  • Pelco UV Cryochamber

How to Start a Project

You can find us at https://cu.corefacilities.org/service_center/5859?tab=about.

To begin a new project, select the Request Services tab, click on “initiate request” button, enter necessary information, and hit "submit." As soon as we have your request, we will contact you to schedule a time to meet and discuss your project.

If you do not have an iLab account, you could complete the registration form on the sign-up page and receive a Welcome Email from iLab with login credentials.

Policies and Procedures


User Fees

The Electron Microscopy Core Facility is a research instrumentation and service facility open to state, university, and external users. Instrument use and technician services are available on a recharge fee basis. Charges help defray the costs of the service contracts, maintenance, salaries, and supplies.

 InternalExternal
Staff Assistance $101 / hour $111 / hour
EM Beam Time $89 / hour $98 / hour
Thick and Thin Sectioning & Post Staining $250 / sample $275 / sample
Sample Preparation >4 samples $160 / sample $176 / sample

 


Acknowledgement

Acknowledgment Requirement:
NIH grant 1S10OD036258-01 must be acknowledged in all publications that include data collected using the JEOL JEM-120i TEM.

Recommended acknowledgment
"This study was supported by the Electron Microscopy Core Facility at CU Anschutz. Data were collected using the JEOL JEM-120i, 120 kV TEM, supported by NIH grant 1S10OD036258-01."
This acknowledgment should also be included in all grant applications that use preliminary data obtained on the new TEM—including training grants—within the Facilities and Other Resources section.

As Needed for Grant Applications

 

Contact

Anza Darehshouri, PhD
[email protected]

Office: 303-724-3098

Lab: 303-724-3450

Department of Cell and Developmental Biology
University of Colorado School of Medicine
12801 E. 17th Ave., RC-1 South
Office: L18-11123
Lab: L18-1207
Aurora, CO 80045

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