IMMUNOASSAYS

Measure proteins and biomarkers in liquid samples

Are you interested in determining the concentration of cytokines, chemokines, or other biomarkers in your human samples? Let us know if you are interested in help with these assays or developing others.

Multiplex Electrochemoluminescent Assays
We offer immunoassays on the MesoScale Discovery (MSD) platform, in which up to 10 different proteins can be measured from one 50 ul sample. MSD has 55 human analytes available on their “V-PLEX” format. All MSD plates are customizable to your analytes of interest. Since you only pay for the analytes you are ordering, you can select just those that reflect your scientific questions. We will run any assay on the MSD platform: mouse, human, rat, non-human primate, you name it!

Running your own MSD assays?
We have a calendar on our iLab page to sign up for time to read your own MSD plates on the SQ120. Our calendar is set up in 15 min increments and we typically read ~3 plates in a 15 minute window. To minimize time between reading and addition of read buffer, users are welcome to perform the final wash step and addition of read buffer in our laboratory. 

ELISpot
We offer ELISpot assays to evaluate the cytokine production of your T cells after stimulation with specific peptides. This assay will determine what antigens your T cells respond or do not respond to.

Image Credit:  https://www.rndsystems.com/products/elispot-assay-principle