FLOW CYTOMETRY
Quantitate the immune cells and marker expression in your sample
Want to quantify or characterize immune cells in biologic fluids or tissues?
At HIMSR, we offer full service flow cytometry. We can stain with an antibody panel of interest, run, and analyze the results of your samples. We can distinguish cellular phenotypes, assess the level of
activation or inhibitory molecules, and measure intracellular signaling
cascades or production of intracellular cytokines. We can also analyze
apoptosis, cell cycle, and cellular assays such as killing and
proliferation. Our staining procedures include compensation controls, fluorescence minus one controls, and a control human PBMC sample. We have pre-developed standard antibody panels and we can also run your pre-stained samples.
We use the instruments in the CU Cancer Center Flow Cytometry Shared Resource
Flow technology is optimal for analysis of any sample in single cell suspension (peripheral blood, PBMCs, disaggregated tissue). The cells can be live, fixed or viably frozen – there are few limitations as long as the antibodies work for your marker of inquiry and sample type.
We are happy to develop new antibody panels to analyze your cells of interest and have standard antibody panels.
- Basic Phenotyping Panel
- T cell Phenotyping Panel
- Monocyte/DC Phenotyping Panel
- T cell Activation Panel
- Lymphocyte Phospho Panel
- Lymphocyte Cytokine Panel
Log into iLabs for service fees; contact Angie Minic for a quote.
