Service | Description | Pricing |
Initial Project Support | Discussion of research project needs, a high-level view of experimental design and timeline, and associated analyses and services | Free |
Experimental Design Support | Design of experiment including number of mice, treatments, time-course and flow cytometry panel design for analysis of Human Immune System mouse tissue | $406 |
Service | Description | Pricing |
BRGS/NBSGW | BRGS (BALB/c Rag2-/- IL2Rγ-/- SIRPαNOD mice) or NBSGW (NOD.CgKitW41J Tyr+ Prkdcscid Il2rgtm1Wjl/ThomJ) | $17 + cage cost |
HIS-CB-BRGS | Human Immune System mice generated from CD34+ HSC’s from cord blood at birth verified by two bleeds to be >25% human, >14 weeks | $364/mouse |
• With full flow immune analysis (tissue harvest and Stains A-D, see below) | $1,394/mouse | |
• With customized flow immune analysis | Contact for pricing | |
HIS-PBMC BRGS | Human Immune System mice generated from peripheral blood mononuclear cells (PBMC) from adult blood or cord blood and injected into adult BRGS immunodeficient mice. Human chimerism verified at 5-14 days post injection. Mice develop GVHD and should be used between 2-4 weeks and typically reconstitute with a predominant activated T cell population and approximately 10% B cells. Model is validated for Cytokine Release Syndrome assays testing inflammatory markers in blood following treatments. | $261/mouse |
HIS-FLT-BRGS | Human Immune System mice using CD34+ fetal liver (FL) hematopoietic stem cells (HSC) and co-implantation of matched thymus. Human Immune System mice are verified for >25% human chimerism in blood. | Contact for pricing |
HIS-BM-BRGS | Human Immune System mice using CD34+ from bone marrow (BM) hematopoietic stem cells (HSC) at birth and verified for >5% human chimerism in blood | Contact for pricing |
Service | Pricing |
Flow Time | $150/hour |
Antibody cost | $4/sample or list price |
Panel design and staining | $133/hour + antibodies + flow time |
Stain A (EC only) (human immune subsets) | $261/mouse |
Stain B (EC only) (HLA, inhibitor receptor expression on tumor and immune cells) | $173/mouse |
Stain C (IC) Tregs, Tem/cm, Granzyme B | $202/mouse |
Stain D (IC) Cytokies (IFNγ, TNFα) | $196/mouse |
Service | Pricing |
HIS Mouse lymph node and spleen harvest | $126/mouse |
HIS Mouse Tissue (eg tumor, lung, liver) harvest | $152/mouse |
Tumor injections | $12/mouse |
Tumor measurement | $12/mouse |
Drug Dosing | $6/mouse |
CD34+ isolation from cord blood | $1,029 per CB |
Julie Lang, PhD
Assistant Director, Human Immune System Mouse Core
303-724-8705
Julie.Lang@cuanschutz.edu