Pancreatic Islet Preparation The DRC Tissue Procurement Core isolates functional pancreatic islets from mice. Other organs of interest (spleen, pancreatic lymph nodes, thymus) may be collected at the same time if desired. While services are requested through iLabs (Barbara Davis Center Bioresource Core), actual scheduling must be done by personally contacting the Core (phone or email).
Islet Harvesting Donor mice (client provides) are humanely euthanized, a collagenase/neutral protease cocktail is injected via the common bile duct, and the enzyme-inflated pancreas is removed. After incubation at 37°C the islets are density-gradient purified from the digested pancreas, and ultimately the clean islets are hand-picked under the microscope. The client may request the end product to be reported as either a simple islet count or scored as islet equivalents (IEQs; 1 IEQ = an islet roughly 150mm square).
Islet / Tissue Transplantation Harvested islets are scored as IEQs and transplanted under the kidney capsule of anaesthetized recipient(s). Recipient mice are also provided by the client; arrangements may be made to house transplanted animals in the BDC Animal Facility. This (kidney capsule) transplantation service is also available for other tissue types (client provides).
Animal Protocol Requirement Researchers requesting pancreatic islets from the Core must first possess a current IACUC-approved animal protocol. A protocol amendment is generally required; I need to be added to your protocol and you will need to include a section on our islet harvest / transplantation procedure(s). I will provide you with my PQF and the appropriate protocol narrative(s).
Human Islet Program The islet core has a Master Material Transfer Agreement (MTA) in place with the Alberta Diabetes Institute. By providing a copy of their Institutional Biosafety Committee (IBC) approval, a brief description of their work, and signing on to the MTA, interested researchers have access to human islets at a cost of $0.10 CAD / IEQ (currently $75 (U.S.)/1000 IEQ plus a $50 (U.S.) shipping charge). Islets are typically offered every week or two. Orders are placed through the Core and are delivered here for distribution.
Scott Beard, Islet isolation manager firstname.lastname@example.org Barbara Davis Center Rm. 4209 303-724-6832 (office) 303-319-8447 (cell)
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