The overall mission of the Tissue Procurement and Processing (TPP) Core is to facilitate access and usage of tissues and services to meet the specialized needs of the diabetes research community to expedite and catalyze innovative diabetes research at UC AMC. We provide resources to assist DRC investigators with the acquisition and processing of rodent and human tissue, and specialized cell lines that are broadly required for diabetes research.
The Tissue procurement and processing core consists of two service sections – (1) Tissue & cell distribution services, and (2) Histology analysis services.
Tissue & Cell distribution
1. Isolation of islets and other tissues from rodents
The following rodent islet isolation services are available.
-Rodent islet & tissue isolation service
-Training for rodent islet isolation
-Reagents for rodent islet isolation – Optimized reagents for islet --isolation such as collagenase are available.
2. Human islets
Human islets are available from the University of Alberta Edmonton Islet Isolation and Distribution Program. The core arranges distribution of human islet samples for registered DRC investigators.
3. Other human tissues
The core arranges access to fresh and viable human tissue specimens with de-identified clinical data (partner with the University of Colorado Biorepository Core Facility in the Department of Pathology).
4. Cell line distribution
1. Embedding, Sectioning & Staining services
We provide tissue fixation, embedding, and sectioning services on request. Standard staining services listed below are available as well.
-Sectioning (paraffin & frozen sections)
-Immunohistochemical Staining (upon request)
2. Access to Instruments
The following instruments are available. The core provides training for usage of instruments upon request.
-Tissue processor (Miles Scientific Tissue-tek VIP)
-Automated embedding system (Leica)
-Cryostats (Microm 325)
-High-throughput quantitative pathological & immunohistochemical -analysis (Perkin Elmer Vectra 3.0) (partner with The University of Colorado Human Immune Monitoring Shared Resource)
3. Technical assistance and training/consultation
Antibodies and other histological reagents that are commonly used for diabetes research such as insulin antibodies are available along with optimized staining protocols.
Maki Nakayama, PhD
Lori Sussel, PhD
For more information about islet islet isolation, islet transplantation and islet procurement services contact:
Barbara Davis Center Rm. 4209
For information on cell line distribution, contact:
For information on histology services, contact: