Cell and Tissue Analysis Core


 

Overview

The Cell and Tissue Analysis Core provides diabetes researchers at the University of Colorado with a unified means to access and effectively use state-of-the art microscopy and mass cytometry systems to drive innovative research. This necessarily includes providing access to this equipment; expertise and training to use the equipment effectively for diabetes-related tissues; expertise and validated diabetes- related resources to prepare samples appropriately; guidance to design experiments; resources to analyze data; and assistance with its interpretation.

MIBI

Advanced Microscopy

Advanced Light microscopy core- The ALMC provides a suite of state of the art microscopy systems with broad capabilities and dedicated professional staff.  

-Multi-color, high sensitivity confocal and two-photon microscopy

            Zeiss LSM780 NLO (with 2-photon excitation and FLIM/FCS)

Olympus FV1000 (with CARS/SRS)

            3I Marianas inverted spinning disk microscope

            3I Vivo upright spinning disk microscope (with 2-photon excitation)

-Super-resolution nanoscopy (STED, STORM/PALM)

            Zeiss Elyria TIRF/STORM

            Home-built STED system (2-color)

-Fluorescence lifetime imaging (metabolism, oxidized lipids, FRET molecular interactions)

-Coherent Raman imaging (CARS/SRS lipid imaging)  

-Subsidized services for diabetes researchers

Reserve an ALMC instrument

Intra-vital microscopy-Imaging systems and expertise is available for custom intra-vital imaging for diabetes applications.

-Abdominal window preparations

-Peripheral nerve preparation

-Intravital imaging and tissue explant imaging

-Upright 2-photon systems

Leica SP8 DIVE (700-1300nm)

Olympus FV1000 with DIVER detector (ultrasensitive detection)

-Whole organ imaging with Light sheet microscope and tissue clearing (coming soon)

 

Reserve an ALMC instrument (DIVER and light sheet)

Request custom intra-vital imaging services

 

Consulting and assistance

-Expert assistance is available for diabetes researchers to incorporate new advanced microscopy approaches to enhance their research. This includes advice on experimental design; technical assistance; assistance with image quantification; and advice for sample preparation.

-We carry specialized reagents needed for certain imaging modalities (e.g. super-resolution imaging), as well as standards for system calibration (e.g. test charts, fluorophore standards).

-The ALMC seminar series introduces new advanced systems and capabilities or covers fundamental concepts in microscopy.

 

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