Documents & Resources
Case report forms have been designed to collect clinical data consistently across sites. All are available directly on the REDCap site if user access has been granted. Individual .pdf documents of unique forms are provided below.
Patients with Burn Injury in a Designated Burn ICU
Subjects with Alcohol Use Disorders (AUDs) and Non-AUD Control Subjects
Patients with Respiratory Failure on Mechanical Ventilation
Active CoPARC Lab Processing and Experimental Methods
Current methods for the processing of CoPARC samples along with methods for the experiments performed in the CoPARC laboratory.
Previous and Specialized CoPARC Lab Methods
Former methods for CoPARC sample processing that are not currently in use but may be relevant to previously collected samples, as well as methods for specialized assays.
The CoPARC Steering Committee directs and distributes all CoPARC data and samples. The committee will prioritize distribution of samples and information based on the following criteria:
- Availability of needed samples and its ease of acquisition
- Quantity and type of samples needed
- Timeframe that investigator requests to receive samples
- Quantity and type of samples previously given to the site
- Study design of proposed experiments with samples
- Funding available to requesting investigator(s) to conduct the project
Necessary regulatory (IRB) approval will be required for proposed projects prior to sites receiving samples or data. The CoPARC Steering Committee may request modifications or clarifications of the proposed protocol prior to its approval based on their review. The committee will make every effort to honor all requests. All data generated through the resources of this consortium will be uploaded to the consortium's main data set in a timely fashion, but may not be accessed without approval from the CoPARC Steering Committee.
In order to help determine priority order of sample distribution, please email the following to coparc@ucdenver.edu:
- Up-to-date biosketch of principal investigator(s)
- CoPARC Sample and Data Request Form
CoPARC Collected Sample Types
| Blood | Bronchoalveolar Lavage (BAL) | Brushings | Other |
|---|---|---|---|
| Serum | Cell Free Upper BAL (Bronchial) | *Microbiome Bronchial Cytobrush | Oral Wash |
| EDTA Plasma (Purple Top) | Cell Free Lower BAL (Alveolar) | Bronchial Cytobrush Cells for RNA | *Microbiome Oral Wash |
| Buffy Coat from EDTA Plasma | Cultured and Uncultured BAL Cell Pellets for RNA/Protein | Bronchial Cytobrush Cell Cytospins, Paraformaldehyde and Methanol Fixed | Urine |
| Heparin Plasma (Green Top) | Cryopreserved Uncultured BAL Cell Pellets | Cryopreserved Cytobrush Cells |
Stool |
| Red Blood Cells from Plasma Tube | Cultured BAL Cell Media | Nasal Brushings Preserved for RNA | |
| Whole Blood from Plasma Tube | Cytospins, Paraformaldehyde or Methanol Fixed | Cryopreserved Nasal Brushings | |
| Cultured and Uncultured Peripheral Blood Mononuclear Cell (PBMC) Pellets | *Microbiome BAL | Nasal Brush Cell Cytospins, Paraformaldehyde and Methanol Fixed |
|
| Cultured PBMC Cell Media | |||
| Cryopreserved PBMCs | *Microbiome = Collected in DNA free conditions. |
Samples Available from Other Consortium Members
Consortium sites that have samples available for sharing. Those sites will update CoPARC with the number and type of samples quarterly so that CoPARC team members can facilitate the shipment of samples from those sites to the requesting investigator(s). The following consortium members are participating:
| Ashish Mehta | Emory University | Longitudinal BAL and Blood from Subjects with AUDs |
| Majid Afshar | Loyola University | BAL and Blood from Patients with Burn Injury |