CoPARC Research Biological Samples

Various types of biologic samples will be obtained from participants enrolled in consortium investigations. Please see table below for complete details.

Ambulatory participants with alcohol use disorders or chronic cannabis use and healthy controls. Enrolled individuals will have sampling conducted in a clinical translational research center (CTRC) or similarly controlled setting. Bronchoscopy with bronchoalveolar lavage (BAL) and brushings will be performed by the principal investigator or her co-investigators, all of whom are pulmonologists.

Critically ill patients with respiratory failure (with and without COVID-19) or with burn injury. Enrolled patients will have data collection and sampling performed in an ICU setting, including information regarding alcohol history and drug use using validated questionnaires. Historical information will generally be collected from a proxy or surrogate of the patient, and will be confirmed by patients once capacity is regained. Data collection, as well as biologic sampling will be performed by trained personnel. Bronchoscopy will be performed by clinical personnel who have experience in performing this procedure.

Description of participant or patient types, and planned sample collection, after screening and informed consent process.

Single bronchoscopy protocolLongitudinal bronchoscopy protocolBurn patient protocolRespiratory failure patient protocol
Participant type(s)AUDs or Chronic Cannabis & Pair-matched controlsAUD participantsBurn ICU patients, ± AUDsRespiratory failure patients in ICU, ± AUDs
Demographic, AUD, smoking (cigarettes and /or cannabis) data collectionYes, from participantYes, from participantYes, from health care proxy, confirmed by patientYes, from health care proxy, confirmed by patient
Length of protocol1 (controls) to 3 (AUDs) days (inpatient)9 days (inpatient)
14 or 21 days (outpatient)
Varies (inpatient)Varies (inpatient)
a Blood (plasma, serum, buffy coat, peripheral blood mononuclear cells)On Day 1On Days 1, 4, and 7Within 24 hours of injury, @Days 3-5, and Days 7-10Within 24 hours of mechanical ventilation, @Days 3-5, and Days 7-10
b Bronchoscopic samplesBAL, cytologic brushings x 3 on Day 1BAL, cytologic brushings x 3 on Day 1; repeat on day 7, 14, or 21BAL, mini-BAL, or tracheal aspirate <48 hours post-intubationBAL, mini-BAL, or tracheal aspirate <48 hours post-intubation
Other sample typesOral wash and nasal epithelial brushing on Day 1Oral wash and nasal epithelial brushing on Days 1 & 7Nasal epithelial brushingNasal epithelial brushing
a Blood volume at each time point=35cc. b BAL yield for ambulatory participants=60cc with 10-20 million WBCs; for critically ill patients=10-20 cc with 10-20 million WBCs. Mini BAL yield=1-4 cc with 1-10 million WBCs.


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