 | Imaging Techniques |
Features
The Zeiss LSM980 is an inverted laser scanning confocal and two photon microscope. It is equipped with a 32-Channel GaAsP QUASAR detector for tunable spectral detection, spectral FCS, and hyperspectral imaging. It also features an Airyscan 2 detector for super resolution imaging, multi-line scanning, and spatial FCS. The tunable femtosecond laser allows for SHG, TEP, FLIM, and FLIM based FRET imaging. Two GaAsP BiG.2 non-descanned detectors are available for sensitive SHG/TPE imaging. The CMOS camera allows large area overviews with epi-fluorescence in the DAPI/FITC/TRITC channels. Paired with the AI Sample Finder, this option offers efficient imaging workflows. The microscope is equipped with a live cell stage insert and a focus stabilization system.
Software
- Zen Blue 3.11
- SymPhoTime 64
- SVI Huygens Essentials
Light Sources
- Laser scanning confocal:
- 405nm, 445nm, 488nm, 514nm, 561nm, and 639nm CW lasers
- SHG/TPE/FLIM:
- SpectraPhysics Mai Tai, pulsed femtosecond laser with tuning range from 690nm – 1040nm
- Epi-fluorescence:
- Excelitas X-Cite XYLIS LED with excitation from 380nm – 770nm
Detection
- Laser Scanning Confocal/TPE/FCS:
- Freely tunable imaging channels from 371nm – 759nm for imaging in up to 3 confocal channels simultaneously (unlimited channels in sequential mode)
- Hyperspectral imaging with QUASAR detection unit with 32 channels in 410nm – 696nm range (possible binwidths: 3nm, 4.4nm, 8.9nm,17.8nm, 26.7nm, 35.5nm, GaAsP detectors), plus 2 PMT detectors in the UV and far-red to extend the spectrum
- Super-resolution imaging with Airyscan 2 detection unit with 8Y Multiplexing for 2x or 8x parallelization. Spatially resolved FCS is possible with the Dynamics Profiler software package
- SHG/2p Fluorescence:
- 2 non-descanned BiG.2 GaAsP detectors with filter cubes for NADH/FAD, SHG/TPE, FITC/TRITC
- Airyscan 2 can be used with 2p fluorescence for super-resolution imaging
- Laser scanning confocal channels are utilizable in same acquisition
- FLIM:
- 2 descanned single photon counting PMA Hybrid 40 mod detectors with a MultiHarp 150 TCSPC card from PicoQuant. Channels: 464nm – 500nm and 524nm – 576nm
- Brightfield/Epi-fluorescence:
- 1 transmitted light PMT channel
- Axiocam 305 mono R2
Objectives
- 2.5x / NA 0.085, Air
- 10x / NA 0.30, Air
- 20x / NA 0.80, Air
- 40x / NA 1.20, Water
- 63x / NA 1.40, Oil
Location: 7th floor RC2, room P15-7244
Acknowledgements
"We would like to thank the personnel from the University of Colorado Anschutz Medical Campus Advanced Light Microscopy Core for imaging assistance. Imaging was performed in the Advanced Light Microscopy Core facility of the NeuroTechnology Center at the University of Colorado Anschutz Medical Campus, which is supported in part by Diabetes Research Center Grant (P30 DK116073). Usage of the Zeiss LSM 980 was supported by S10 OD036220."
