Immune Monitoring Services
As an academic business, our lab integrates a scholarly mission to contribute to the advancement of science and medicine with high quality immune monitoring services. We have assisted numerous academic and industry researchers across the world with consultation, research and development, validation, and data analysis of flow cytometry and immune assays. With access to an array of state of the art instrumentation and staffed by experienced personnel, our lab is able to combine academic expertise with industry standards of efficiency and rigor in our services.
Immune Monitoring Services:
Multicolor Flow Cytometry Assays
- Surface Marker Staining
- Intracellular Cytokine Staining
- Transcription Factor Staining
Ex Vivo Assays
- PMA/Ionomycin, SEB, LPS
- Cell Proliferation - CFSE, Celltrace
- Cell Receptor Blockade, i.e. anti-TLR
Cell Culture
- Cell Lines
- Cell Differentiation
Cytokine Production
- ELISAs, Multiplex MSD
- ELISPOT
In Vitro Assays
- PCR, qPCR
- RNA/DNA Extraction
Consultation
- Flow Panel and Assay Development
- Data Analysis, Reporting, and Interpretation
We offer standard, previously validated flow cytometry panels, as well as custom built panels most relevant to your desease model or field of interest. For inquiries, please contact Brent Palmer at Brent.Palmer@cuanschutz.edu
Mouse Tissue Processing:
- Spleen
- Lymph Nodes
- Ileum and Colon Tissue
- Lung
- Blood
- Kidney
- Bone Marrow
- Paws, Ears
Mouse Models we have previously worked with:
- Collagen-Induced Arthritis
- Adoptive Transfer IBS
- Xenograft GVHD
- NZM and MRL/lpr Lupus Models
- Retroviral Infection
- Gnotobiotic FMT
Sample Validated Flow Cytometry Panels
Flow Cytometry panels shown below are examples that have been validated for previous projects. All panels are modifiable and can be optimized for various tissues and disease models. New panels can also be designed and validated.
Sample Flow Plots
Lupus NZB Model - Spleen B Cell Panel
Lupus NZB Model - Spleen T Cell Activation Panel
Adoptive Transfer IBS Model - Colon ICCS
Human Tissue Processing:
- Blood
- Small and Large Intestine
- Bronchoalveolar Lavage
- Lymph Node/Tonsils
Sample Flow Plots
Human Jejunum Lamina Propria Cells - T Cell Activation
NHP Tissue Processing:
- Blood
- Bone Marrow
- Spleen
- Lymph nodes (different lymph nodes and Fine needle aspiration lymph node)
- Gut tissues (jejunal and rectal)
- Liver
- Lung
- Brochoalveolar Lavage Fluid
- Nasal Wash Fluid
- Cervical Cytobrush Fluid
- Cervicovaginal Lavage Fluid
Flow Core
Scheduler
Cell Analyzers
Cell Sorters
Immune Monitoring Services
Biorepository and Cell Banking
Clinical Testing
Our Team
Forms, Policies and Procedures
Accreditation
Hours of Operation
Staffed: Monday to Friday (9am to 5pm)
24/7 for Trained Users for analyzers
Location
University of Colorado Anschutz Medical Campus
Allergy & Clinical Immunology/School of Medicine
Research Complex 2 (10th Floor)
12700 E. 19th Ave, Rm # 10232, Aurora, CO 80045
Contact Information
Brent Palmer, PhD
Director
(303)-724-7203
Dillon Flebbe
Flow Core Manager
303-724-7211
DILLON.FLEBBE@CUANSCHUTZ.EDU