Immune Monitoring Services


 

As an academic business, our lab integrates a scholarly mission to contribute to the advancement of science and medicine with high quality immune monitoring services. We have assisted numerous academic and industry researchers across the world with consultation, research and development, validation, and data analysis of flow cytometry and immune assays. With access to an array of state of the art instrumentation and staffed by experienced personnel, our lab is able to combine academic expertise with industry standards of efficiency and rigor in our services.​

Immune Monitoring Services:

Multicolor Flow Cytometry Assays

  • Surface Marker Staining
  • Intracellular Cytokine Staining
  • Transcription Factor Staining

 

Ex Vivo Assays

  • PMA/Ionomycin, SEB, LPS
  • Cell Proliferation - CFSE, Celltrace
  • Cell Receptor Blockade, i.e. anti-TLR

 

Cell Culture

  • Cell Lines
  • Cell Differentiation

 

Cytokine Production

  • ELISAs, Multiplex MSD
  • ELISPOT

 

In Vitro Assays

  • PCR, qPCR
  • RNA/DNA Extraction

 

Consultation

  • Flow Panel and Assay Development
  • Data Analysis, Reporting, and Interpretation

 

We offer standard, previously validated flow cytometry panels, as well as custom built panels most relevant to your desease model or field of interest. For inquiries, please contact Sharon Sen at Sharon.Sen@ucdenver.edu

       

Mouse Tissue Processing:

  • Spleen
  • Lymph Nodes
  • Ileum and Colon Tissue
  • Lung
  • Blood
  • Kidney
  • Bone Marrow
  • Paws, Ears

 

Mouse Models we have previously worked with:

  • Collagen-Induced Arthritis
  • Adoptive Transfer IBS
  • Xenograft GVHD
  • NZM and MRL/lpr Lupus Models
  • Retroviral Infection
  • Gnotobiotic FMT

 

Sample Validated Flow Cytometry Panels

 

​Flow Cytometry panels shown below are examples that have been validated for previous projects. All panels are modifiable and can be optimized for various tissues and disease models. New panels can also be designed and validated.

Collagen Model

Adoptive Model

Xenograft Lupus Model

Sample Flow Plots

Lupus NZB Model - Spleen B Cell Panel

Lupus NZB Model - Spleen B Cell Panel

Lupus NZB Model - Spleen T Cell Activation Panel

Lupus NZB Model - Spleen T Cell Activation Panel

Adoptive Transfer IBS Model - Colon ICCS

Adoptive Transfer IBS Model - Colon ICCS

Human Tissue Processing:

  • Blood
  • Small and Large Intestine
  • Bronchoalveolar Lavage
  • Lymph Node/Tonsils

Sample Flow Plots

​Human Jejunum Lamina Propria Cells - T Cell Activation​ 

​Human Jejunum Lamina Propria Cells - T Cell Activation​

Human PBMC, PMA/Lonomycin Activated, Intracellular Cytokine Staining​
Human PBMC, PMA/Lonomycin Activated, Intracellular Cytokine Staining​