Immune Monitoring Services


As an academic business, our lab integrates a scholarly mission to contribute to the advancement of science and medicine with high quality immune monitoring services. We have assisted numerous academic and industry researchers across the world with consultation, research and development, validation, and data analysis of flow cytometry and immune assays. With access to an array of state of the art instrumentation and staffed by experienced personnel, our lab is able to combine academic expertise with industry standards of efficiency and rigor in our services.​

Immune Monitoring Services:

Multicolor Flow Cytometry Assays

  • Surface Marker Staining
  • Intracellular Cytokine Staining
  • Transcription Factor Staining


Ex Vivo Assays

  • PMA/Ionomycin, SEB, LPS
  • Cell Proliferation - CFSE, Celltrace
  • Cell Receptor Blockade, i.e. anti-TLR


Cell Culture

  • Cell Lines
  • Cell Differentiation


Cytokine Production

  • ELISAs, Multiplex MSD


In Vitro Assays

  • PCR, qPCR
  • RNA/DNA Extraction



  • Flow Panel and Assay Development
  • Data Analysis, Reporting, and Interpretation


We offer standard, previously validated flow cytometry panels, as well as custom built panels most relevant to your desease model or field of interest. For inquiries, please contact Brent Palmer at


Mouse Tissue Processing:

  • Spleen
  • Lymph Nodes
  • Ileum and Colon Tissue
  • Lung
  • Blood
  • Kidney
  • Bone Marrow
  • Paws, Ears


Mouse Models we have previously worked with:

  • Collagen-Induced Arthritis
  • Adoptive Transfer IBS
  • Xenograft GVHD
  • NZM and MRL/lpr Lupus Models
  • Retroviral Infection
  • Gnotobiotic FMT


Sample Validated Flow Cytometry Panels


​Flow Cytometry panels shown below are examples that have been validated for previous projects. All panels are modifiable and can be optimized for various tissues and disease models. New panels can also be designed and validated.

Collagen Model

Adoptive Model

Xenograft Lupus Model

Sample Flow Plots

Lupus NZB Model - Spleen B Cell Panel

Lupus NZB Model - Spleen B Cell Panel

Lupus NZB Model - Spleen T Cell Activation Panel

Lupus NZB Model - Spleen T Cell Activation Panel

Adoptive Transfer IBS Model - Colon ICCS

Adoptive Transfer IBS Model - Colon ICCS

Human Tissue Processing:

  • Blood
  • Small and Large Intestine
  • Bronchoalveolar Lavage
  • Lymph Node/Tonsils

Sample Flow Plots

​Human Jejunum Lamina Propria Cells - T Cell Activation​ 

​Human Jejunum Lamina Propria Cells - T Cell Activation​

Human PBMC, PMA/Lonomycin Activated, Intracellular Cytokine Staining​
Human PBMC, PMA/Lonomycin Activated, Intracellular Cytokine Staining​


 NHP Tissue Processing:

    • Blood
    • Bone Marrow
    • Spleen
    • Lymph nodes (different lymph nodes and Fine needle aspiration lymph node)
    • Gut tissues (jejunal and rectal)
    • Liver
    • Lung
    • Brochoalveolar Lavage Fluid
    • Nasal Wash Fluid
    • Cervical Cytobrush Fluid
    • Cervicovaginal Lavage Fluid

Flow Core


Cell Analyzers

Cell Sorters

Immune Monitoring Services

Biorepository and Cell Banking

Clinical Testing

Our Team

Forms, Policies and Procedures


Hours of Operation

Staffed: Monday to Friday (9am to 5pm)

24/7 for Trained Users for analyzers



University of Colorado Anschutz Medical Campus

Allergy & Clinical Immunology/School of Medicine

Research Complex 2 (10th Floor)

12700 E. 19th Ave, Rm # 10232, Aurora, CO  80045


Contact Information

Brent Palmer, PhD



Henry Chu

Flow Core Manager 




CMS Login