Olympus FV1000MPE upright multiphoton (DIVER) 

 (room P15-7251, RC2)

• Laser scanning confocal (filter-based) and multi-photon
• Lasers lines: 458nm, 488nm, 514nm, 543nm, 633nm, multiphoton SpectraPhysics Insight X3, tunable 680-1300nm and 1045nm
• DIVER detection for FLIM, SHG, THG.

Olympus FV1000 FCS/RICS

 (room P15-7251, RC2)

• confocal laser scanning imaging
• lasers: 405nm, 458nm, 488nm, 515nm, 543nm, 635nm
• CO2/humidity/temperature controlled chamber
• non-descanned detectors for two-photon, SHG and CARS
• capable of FCS, RICS and CARS

 note: RESTRICTED USE for CARS, only with ALMC personnel assistance

Leica DM6000B CTR6000 Slide Scanner

(room P18-7402A2, RC1)

• fluorescence widefield and brightfield imaging of the whole slide
• motorized stage with 4 slides holder
• high sensitivity imaging with DFC365FX camera
• 3 channels: dapi, fitc, tritc

ISS K2 Photon Counting Spectrofluorometer 

 (room P15-7244, RC2)

• corrected emission and excitation spectra
• fast and slow kinetics
• polarization and ratiometric measurements
• temperature control of sample

OLYMPUS IX81 inverted widefield/brightfield 

 (room P15-7243, RC2)

• brightfield (DIC) and widefield fluorescence
• 5MP color camera
• uses AmScope software for image acquisition

OLYMPUS IX81 inverted motorized Microscope 

 (room P18-7402C, RC1)

• Olympus OBJECTIVES- U PLAN APO 10X oil N.A. 0.40, UAPO 40X oil N.A. 1.35, TIRFM PLAN APO 60X oil N.A. 1.45 WD 0.15mm, U PLAN APO 100X oil N.A. 1.35.
• EXCITATION SOURCE: 100 watt Hg lamp.
• CAMERA; Hamamatsu ORCA IIER monochromatic CCD camera 1344X1024 full chip, 6.45X6.45 um pixel .
• FILTER SETS: DAPI EX360-70 EM420-60, FITC EX450-80 EM535, TRITC EX535 EM635; CFP/YFP FRET filter set 8600 2v2(JP4)
• PLATFORM: Dell P4 3.2 GHz, Windows 2000 .
• DATA: ethernet, CD-W or DVD-W 16X.
• Acquisition SOFTWARE: Intelligent Imaging Slidebook v.4.067.

 Super Resolution 3D PALM/STORM and TIRF, Zeiss Elyra P.1  

 (room P18-7402A1, RC1)

• lasers: 488nm 200 mW OPS Laser, 405nm Diode Laser 50mW, 561nm Laser 200 mW OPS and 640nm Diode Laser 150 mW
• camera: Andor 897 EMCCD
• software: ZEN2012 with MultiEmitter functionality (allows for the fitting of overlapping events)
• Zeiss Axio Observer Z1 inverted microscope with environmental chamber and Definite Focus for drift correction during live cell TIRF imaging
• 3D super-resolution (20nm laterally and 50nm axially) via Phase Ramp Localization Imaging Microscopy (PRLIM) and Monte Carlo simulation for signal/noise axial localization

3I MARIANAS inverted Spinning Disk

(room P18-7402A2, RC1)

• fast confocal imaging on Zeiss Axio Observer Z1 with Yokogawa CSU-X1
• lasers: 405, 445, 488, 515, 561 nm lines with millisecond 3I mSwitcher
• 3I mSAC millisecond spherical aberation correction
• 3I live specimen Vector Scanner for photo-manipulation
• Photometrics Evolve 16-bit EMCCD camera for confocal imaging
• Photometrics CoolSNAP HQ2 camera for wide-field imaging and FURA2 ratiometric Ca imaging
• Okolab cage incubator with temperature, CO2, air and humidity control
• Definite Focus for drift correction during live cell imaging
• uses 3I Slidebook 5.5 and/or 6.0 software

3I VIVO Upright Spinning Disk with 2P

(room P18-7206, RC1)

• fast confocal imaging on Zeiss Axio Examiner D1 withYokogawa CSU-X1
• lasers: 405, 488, 561 nm lines with millisecond 3I mSwitcher
• Photometrics Evolve 16-bit EMCCD camera
• 3I live specimen Vector Scanner for photo-manipulation and two-photon imaging
• two-photon imaging via GaAsP non-descanned detection using MIRA900 (Coherent) tunable laser (700-900nm)
• available 4.5mm long working distance objective LD Scale Plan-Apochromat 20X NA1.0 for deep imaging of "Scale" cleared tissue
• uses 3I Slidebook 5.5 and/or 6.0 software

Two-color STED Microscope

(room L18-11216, RC1-South)

• Super-resolution microscope using STimulated Emission Depletion (STED) that can allow resolution down to ~40-50 nm.
• Two-color channel super-resolution imaging using Atto 590 (STAR 580) and Atto 647N (STAR 635 or STAR 635P) fluorophores.
• Built around an Olympus IX-71 microscope with bright field, DIC and epi-fluorescence imaging of the same field of view as STED imaging.
• Must have Dominik Stich present to do imaging.

Zeiss LSM780 spectral, FLIM, 2P, SHG, FCS/RICS

(room P15-7244, RC2)

• Spectral unmixing confocal and two-photon imaging
• 34-Channel GaAsP QUASAR Detection Unit and non-descanned detectors for two-photon fluorescence and second harmonic generation
• Visible lasers lines: 458nm, 488nm, 514nm, 561nm, 633nm
• Tunable infrared Coherent Chameleon Ultra II laser (680nm–1080nm)
• FLIM (lifetime imaging)
• Temperature controlled stage
• Definite Focus for drift correction during live cell imaging
• ZEN2011 software with FCS/RICS package