Program

Tuesday, August 1

6:30 AM - 8:00 AM

Breakfast (Included with Embassy Suites Reservation)

Breakfast Buffet

Session 1

8:00 AM - 10:00 AM

Retroviral Integrases: Structure and Function

Chairperson: Monica Roth, Rutgers University

East End/West End Conference Room

15

Robert Craigie

NIH, Bethesda, MD, USA.

HIV Intasomes: Where we are and where we are going.

16

Dmitry Lyumkis

The Salk Institute for Biological Studies, La Jolla, CA, USA.

Implications for Integrase Functional Plasticity from the Structure of the HIV-1 Integrase Tetramer.

17

Krishan Pandey

Saint Louis University, MO, USA.

Molecular determinants of the Rous sarcoma virus intasome assembly.

18

Kushol Gupta

University of Pennsylvania, Philadelphia, PA, USA.

Structural Consequences of Resistance Mutations on the Formation of ALLINI-Induced Branched Polymers of HIV-1 Integrase.

10:00 AM - 10:20 AM

Coffee Break

Foyer

10:20 AM - 12:20 PM

19

Kristine Yoder

The Ohio State University, Columbus, OH, USA.

DNA Strand Breaks and Gaps Target PFV Intasome Binding and Catalysis.

East End/West End Conference Room

20

Chandravanu Dash

Meharry Medical College, Nashville, TN, USA.

An optimal substrate for HIV-1 preintegration complex mediated viral DNA integration.

21

Stephen Goff

Columbia University Medical Center, NY, USA.

Working overtime: Jump-starting provirus transcription, redirecting sites of integration, and activating DNA damage repair pathways.

22

Wesley Sundquist

University of Utah School of Medicine, Salt Lake City, UT, USA.

Reconstitution and characterization of a cell-free system for HIV-1 capsid-dependent replication and integration.

12:20 PM - 1:30 PM

Lunch

Brickstones Kitchen and Bar

Session 2

1:30 PM - 3:30 PM

Integrase-Host Factor Interactions

Chairperson: Marina Lusic, University Hospital Heidelberg

East End/West End Conference Room

23

Monica Roth

Robert Wood Johnson Medical School, Rutgers, Piscataway, NJ, USA. 

Studies of the common binding motif BRD3 ET domain: polymorphic structural interfaces with host/viral proteins and small molecules. 

24

Goedele Maertens

Imperial College London, United Kingdom.

Investigating the role of PP2A-B56 in establishing HTLV-1 infection. 

25

Ganjam Kalpana

Albert Einstein College of Medicine, New York, NY, USA. 

INI1/SMARCB1 IN binding domain mimicry to TAR RNA and its influence on viral late events and particle morphogenesis: Development of novel class of INI1-derived inhibitors

26

Marina Lusic

University Hospital Heidelberg, Germany.

HIV-1 integration into R-loop enriched genomic regions is mediated by Aquarius helicase of the Intron Binding Complex.

3:30 PM - 3:50 PM

Coffee Break

Foyer

3:50 PM - 5:05 PM

27

Henry Levin

Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD. 

The role of LEDGF in transcription is intertwined with its function in HIV-1 integration. 

East End/West End Conference Room

28

Selected Short Talks

Joshua Hope

The Francis Crick Institute, London, UK. 

The rules of engagement between lentiviral integration machinery and chromatin. 

29

Ross Larue

The Ohio State University, Columbus, OH, USA.  

Single molecule visualization of intasome assembly. 

30

Arpa Hudait

University of Chicago, IL, USA. 

Multiscale simulations of HIV-1 capsid nuclear entry and host factor interactions. 

5:05 PM - 6:30 PM

Evening Reception (included with Embassy Suites Reservation)

Brickstones Kitchen and Bar

6:30 PM - 9:00 PM

Networking Event at Avanti: Outside Flatiron Rooftop

Participants should walk or Uber/Lyft

1401 Pearl Street, Boulder, CO 80302 

Program

Wednesday, August 2

6:30 AM - 8:15 AM

Breakfast (Included with Embassy Suites Reservation)

Breakfast Buffet

8:30 AM - 1:00 PM

Excursion to the Rocky Mountain National Park

Busses depart from the Embassy Suites at 8:30 AM 

Session 3

2:00 PM - 3:00 PM

Nuclear Import of HIV-1 Core S/Preintegration Complexes

Chairperson: Melissa Kane, University of Pittsburgh

East End/West End Conference Room

31

Hans-Georg Kräusslich

Heidelberg University, Heidelberg, Germany. 

Capsid as key orchestrator of early HIV-1 replication.

32

Vinay Pathak

HIV DRP, NCI-Frederick, MD, USA. 

Mechanisms of HIV-1 Core Uncoating, Nuclear Import Kinetics, and Integration Site Selection. 

3:00 PM - 3:20 PM

Coffee Break

Foyer

3:20 PM - 5:20 PM

33

Kate Bishop

The Francis Crick Institute, London, UK.

HIV-1 requires capsid remodelling at the nuclear pore for nuclear entry and CPSF6 binding.

East End/West End Conference Room

34

Edward Campbell

Loyola University Chicago, IL, USA. 

Distinct utilization of nuclear import pathways allows HIV-1 integration into transcriptionally active regions of the chromatin.

35

Ashwanth Francis

Florida State University, Tallahassee, FL, USA. 

Live-cell imaging of HIV-1 nuclear transport and association with nuclear speckles.

36

Selected Short Talks

João Mamede

Rush University Medical Center, Chicago, IL, USA. 

Fluorescent labeled CA correlates progressive uncoating from the cytoplasm to the nucleus to productive HIV infection in primary cells.

37

Melissa Kane

University of Pittsburgh, Pittsburgh, PA, USA. 

Effects of the cyclophilin homology domain of RanBP2 on HIV-1 infection and Mx2 activity. 

5:20 PM - 6:30 PM

Evening Reception (included with Embassy Suites Reservation)

Brickstones Kitchen and Bar

6:30 PM - 8:00 PM

Gala Dinner at the Embassy Suites Courtyard

Outdoor Courtyard

8:00 PM - 9:00 PM

"Where Do You See Your Future?"

The discussion panel:

Goedele Maertens, Imperial College London (moderator)

Stephen Yant, Gilead Sciences

Suzanne Sandmeyer, University of California Irvine

Mark Underwood, ViiV Healthcare

Wesley Sundquist, University of Utah

East End/West End Conference Room

Program

Thursday, August 3

6:30 AM - 8:00 AM

Breakfast (Included with Embassy Suites Reservation)

Breakfast Buffet

Session 4

8:00 AM - 10:00 AM

HIV-1 Integrase Inhibitors and Novel Antiretroviral Compounds

Chairperson: Daniel Adu-Ampratwum, The Ohio State University

East End/West End Conference Room

38

Kyungjin Peter Kim

ST PHARM, Seoul, Republic of Korea. 

The Fellowship of the Ring: Quest to develop Pirmitegravir, a novel potent and safe HIV-1 allosteric integrase inhibitor (ALLINI). 

39

Jacques Kessl

University of Southern Mississippi, Hattiesburg, MS, USA. 

Optimizing the binding of substituted quinoline ALLINIs within the HIV-1 integrase oligomer. 

40

Stephen Yant

Gilead Sciences, Inc., Foster City, CA, USA. 

Lenacapavir: A First-in-Class, Long-Acting HIV Capsid Inhibitor for Treatment and Prevention. 

41

Daniel Adu-Ampratwum

The Ohio State University, Columbus, OH, USA. 

Developing novel small molecules as inhibitors targeting HIV-1 Integrase and capsid proteins. 

10:00 AM - 10:20 AM

Coffee Break

Foyer

10:20 AM - 12:20 PM

42

Eric Gillis

Discovery Chemistry, ViiV Healthcare, Branford, CT, USA. 

Potent long-acting inhibitors targeting HIV-1 capsid based on a versatile quinazolin-4-one scaffold. 

East End/West End Conference Room

43

Mark Underwood

ViiV Healthcare, Durham, North Carolina, USA. 

Second Generation Integrase Inhibitor Resistance in the Clinic: Dolutegravir Resistance Mechanisms and Structural Underpinnings. 

44

Selected Short Talks

Yuta Hikichi

HIV Dynamics and Replication Program, NCI-Frederick, MD, USA. 

Mutations outside integrase lead to high-level resistance to integrase strand transfer inhibitors. 

45

Jose Dekker

Amsterdam UMC - University of Amsterdam, Amsterdam, The Netherlands.

HIV-1 3'-polypurine tract mutations confer dolutegravir resistance by switching to an integration-independent replication mechanism via 1-LTR circles. 

46

Roberto DiSanto

Sapienza University of Rome, Rome, Italy.

New small molecule derivatives as dual inhibitors of the HIV-1 integrase catalytic site and integrase-RNA interactions. 

47

Szu-Wei Huang

University of Colorado, AMC, Aurora, CO, USA. 

Sub-stoichiometric drug to HIV-1 capsid ratio enables ultra-potent antiretroviral activity of lenacapavir. 

12:20 PM - 1:30 PM

Lunch

Brickstones Kitchen and Bar

Session 5

1:30 PM - 3:30 PM

Poster Presentations

61-83

Outdoor Courtyard

Session 6

3:30 PM - 5:30 PM

Retrotransposons and Serine Integrases

Chairperson: Kate Bishop, The Francis Crick Institute

East End/West End Conference Room

48

Susan Sandmeyer

University of California, Irvine, CA, USA.

Ty3: We should have known it wouldn't be random.

49

 David Garfinkel

University of Georgia, Athens, GA.

Ty1 Gag stories: mechanism of copy number control, domestication of a restriction factor, and an inerchangeable prion-llike domain. 

50

Phoebe Rice

The University of Chicago, IL, USA.

Large serine integrases: how do they know which way to go?

51

Selected Short Talks

Eric Arts

Western University, London, Ontario, Canada.

Evidence of significantly reduced HIV proviral integrants within genes and increased integration into transcriptionally silent elements in HIV-1 infected individuals failing an INSTI treatment regimen with or without INSTI resistance mutations. 

52

Ariberto Fassati

Institute of Immunity and Transplantation, London, UK.

Functional mapping of integration sites connected to latent HIV-1 infection. 

5:30 PM - 6:30 PM

Evening Reception (included with Embassy Suites Reservation)

Brickstones Kitchen and Bar

6:30 PM -

Special Dinner Reception at The Flagstaff House

Busses depart from Embassy Suites at 6:30 PM.

Program

Friday, August 4

6:30 AM - 8:00 AM

Breakfast (Included with Embassy Suites Reservation)

Breakfast Buffet

8:00 AM - 8:10 AM

Awards for best poster presentations and short talks presented by Kristine Yoder

East End/West End Conference Room

Session 7

8:10 AM - 10:10 AM

Retroviral Integration Site Selectivity

Chairperson: Mary Kearney, HIV Dynamics & Replication Program

53

Frederic Bushman

University of Pennsylvania, Philadelphia, PA, USA.

Retroviral DNA Integration: Target Site Selection and Genomic Consequences. 

54

Charles Bangham

Imperial College London, London, United Kingdom.

HTLV-1 integration site: impact on viral persistence and host chromatin structure and expression.

55

Alan Engelman

Dana-Farber Cancer Institute, Boston, MA, USA. 

CPSF6 Liquid-Liquid Phase Separation Determines Higher-Order Capsid Binding, Nuclear Core Incursion, and HIV Integration Targeting.

56

Zeger Debyser

KU Leuven, Flanders, Belgium.

The chromatin landscape of the HIV provirus determines its transcriptional state. Implications for a functional block-and lock cure strategy.

10:10 AM - 10:30 AM

 Coffee Break

Foyer

Session 8

10:30 AM - 12:30 PM

Latency

Chairperson: Eric Arts, Western University

East End/West End Conference Room

57

Mary Kearney

HIV Dynamics & Replication Program, NCI-Frederick, MD, USA.

Divergent Propulations of Infected Naive and Memory CD4+ T Cells Clones in Children on ART.

58

Mathias Lichterfeld

Harvard Medical School, Boston, MA, USA.

Chromosomal Integration sites as biomarkers of HIV-1 reservoir cell selection.

59

Frank Maldarelli

HIV DRP, NCI-Frederick, MD, USA. 

Anatomic Distribution of HIV-Infected Cells After Long Term Antiretroviral Therapy.

60

Duane Grandgenett

Saint Louis University, St. Louis, MO. 

Concluding remarks: retrovirus integrase, integration, HIV-1 integrase inhibitors.

12:30 PM - 2:00 PM

Lunch & Departure

Brickstones Kitchen and Bar