2021-2022 NTC HighlightsIn 2022 the NTC consolidated a number of the operations for Optogenetics and Neural Engineering (ONE) Core and the Advanced Light Microscopy Core (ALMC), in the newly renovated space located on the northern 1st floor interior corridor of building RC1 North.
-Total usage of the core: 4461 hours
Advanced Light Microscopy (ALMC) Core Highlights
-Total number of laboratories that have used the core: 90
-Number of new users of the core: 85
-Number of papers published that acknowledge usage of the core: 11
-New microscope purchased: STEDYCON by Abberior, arriving next fiscal year
-Submitted NIH S10 grant for new STED super-resolution microscope system
-Served 18 research laboratories working on ~40 projects.
Optogenetics and Neural Engineering (ONE) Core Highlights
-Developed an automated system for objectively quantifying motor deficits in patients.
-Developed software to obtain timestamped recordings of multiple, hardware-independent videos for human subject and animal research behavior tasks.
-Engineered methods for synchronization of multiple concurrent behavioral tasks including data pipelines for defining unique timelines across all systems, utilizing commonly used communication systems.
-Designed and fabricated electronic circuits for generation of novel predefined analog signals for simultaneous optogenetic stimulation and electrophysiological recording.
-Served 8 research laboratories.
Innovation and Design for Experimentation and Analysis (IDEA) Core Highlights
-Co-authored three peer-reviewed publications and one book chapter.
-LocoMouse - Visual assessment and quantification of mouse ataxia
-RotoRodent - Quantifying optomotor response of mice and rats in the Animal Behavior Core
-Jump-Reach - Variable reach distance apparatus for head-fixed mice
-Vari-Reach - Real-time repositioning of target for head-fixed mouse reach
-Angular stereotax - Novel apparatus for positioning up to eight mouse brain probes
-The shop serviced over 110 projects last year from 47 different departments.
Neuroscience Machine Shop Highlights
-These projects included new fabrication of research equipment like innovative hexagonal maze systems, maintenance of existing equipment, and repair projects for damaged equipment and animal enclosures.
-Fabricated an intricate tungsten array for Radiation Oncology.
-Designed and fabricated two large acoustical isolation sound chambers.
-Produced a novel behavioral observation rig with probe commutators and cameras.
-Helped research teams design, plan, and perform long-term and short-term behavioral studies.
Animal Behavior Core (ABC) Highlights
-Instituted new behavioral testing paradigms (Dynamic Weight Bearing, Gait Analysis, Home Cage Monitoring)
-Added Zebra Fish Behavior subcore in R2 vivarium
-Total number of laboratories that used the ABC: 25 (67% increase)
-School of Medicine departments/divisions served: 10
-4 published papers and 5 papers submitted to journals.
-Continued to develop and sciatic nerve recording and analysis services.
In Vivo Neurophysiology Core (IVNC) Highlights
-Created custom made visual evoked potential stimulus control boxes and Python code for data analysis
-Continued to refine electrode placement verification service in collaboration with the ALMC
-Trained new PIs on mouse stereotaxic surgery and stereotaxic-guided injections
-Added new EEG recording data analysis service
-Total number of laboratories that used the IVNC: 9 (no change)
-School of Medicine departments/divisions served: 7 (17% increase)