Muscle tissue obtained at biopsy is prepared for frozen sectioning. Frozen sections of muscle tissue are routinely prepared using numerous different histochemical stains. The standard staining battery on frozen tissue includes:
- H&E
- Modified trichrome
- Verhoeff Van Gieson
- Acid Phophatase
- Esterase
- ATPase (pH 9.4 and 4.6)
- NADH-TR
- PAS
- Oil Red O
- Cytochrome oxidase
Additional muscle tissue is routinely archived for future analyses.