University of Colorado Anschutz Medical Campus

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Pilot Grant Program

APPLICATIONS FOR THE FALL 2022 RBI PILOT GRANT PROGRAM ARE CLOSED!

CHECK BACK IN JANUARY 2023 FOR OUR NEXT RFA.

Program Statistics 2016-2021

120 RBI Pilot Grants Awarded

67 projects completed
28 projects are still in progress/have not yet started
4 grants were abandoned due to insurmountable technical difficulties
21 awardees yet to report the outcomes of their award(s)

103 Investigators Participated

3 AMC Schools- School of Medicine, School of Dental Medicine, Skaggs School of Pharmacy
16 Departments
8 Medicine Divisions
5 Pediatric Divisions
2 Surgery Divisions

Total Funds Spent by RBI = $2,488,631

Gross Return by Investigators = $56,540,451

Net Return to AMC = $54,051,820

Fold Increase = 21.72

Return On Investment (ROI) = 2172%

Thanks for the support! It truly was “transformative”. Data from our 2017 RBI Pilot Grant project supported 2 publications and an NIH R35 for $6,904,527 in total costs.

Bruce Appel, PhD

Section Head, Professor of Pediatrics, Diane G. Wallach Chair of Pediatric Stem Cell Biology

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Program Impacts 2016-2021

Financial-

40 new grants funded using data/findings derived from RBI Pilot Grants (28 NIH, 2 NSF, 1 DOD, 9 Other)
6 R01’s PENDING with fundable scores
16 additional grants have been submitted (NIH R01, NIH R21, NSF, DOD, Cancer Center, societies/foundations)
15 grants in preparation

Pilot Funding Graph

Visibility-

34 publications in 27 different journals
7 submitted manuscripts
31 manuscripts in preparation
3 patent applications
Collaborations with CU Boulder, Arizona State, University of Tennessee, University or Iowa, and others

RECIPIENT	DEPARTMENT	AWARD	PROJECT
Santos Franco, PhD	Pediatrics- Developmental Biology	Spatial Transcriptomics	Elucidating the spatiotemporal mechanisms of neural stem cell fate specification in the developing brain
Katharina Hopp, PhD	Medicine- Renal Diseases and Hypertension	Spatial Transcriptomics	Utilizing spatial transcriptomic profiling to understand the heterogenous histopathology of polycystic kidney disease
Eric Clambey, PhD	Immunology & Microbiology	mRNA-LNP	mRNA-LNP vaccination against chronic gammaherpesvirus infection
Heide Ford, PhD	Pharmacology	Ribosome Profiling	Determining the Mechanism of Action of 8430, a Novel Inhibitor of Metastasis
Matthew Gailbraith, PhD	Pharmacology	Nanopore	Long-read RNA-sequencing to discover novel transcriptome features in Down syndrome
Jeffrey Kieft, PhD	BMG	Nanopore	Using long-read NGS to interrogate flavivirus noncoding RNA structure and modifications
Matthew Taliaferro, PhD	BMG	Nanopore	Nanopore-based detection of 8-oxoguanosine for RNA localization analysis

RECIPIENT	DEPARTMENT	AWARD	PROJECT
David Bentley	BMG	RNA-seq	Determination of nascent RNA folding in vivo by psoralen cross-linking
James Maloney	Medicine- Pulmonary Sciences and Critical Care	RNA-seq	Gene expression changes in human T-regs in response to the SARS-CoV-2 nsp15 protein
Tania Reis	Medicine- Endocrinology, Metabolism, and Diabetes	RNA-seq	Dissecting m6A's role in metabolism
Chandra Tucker	Pharmacology	RNA-seq	Probing RNA transcriptional responses to sudden appearance or dissolution of biomolecular condensates
Ethan Hughes	CDB	scRNA-seq	Determine the transcriptomics profile of mature oligodendrocytes participating in remyelination
Elena Hsieh	Pediatrics-Allergy/Immunology	scRNA-seq	Understanding SARS-CoV-2 B cell responses derived from COVID-19 immunization
Suja Jagannathan	BMG	scRNA-seq	Singe cell long-read CaptureSeq to characterize isoform structure of DUX4 transcripts
Matt Kennedy	Pharmacology	scRNA-seq	Profiling amyloid-beta binding cells of the central nervous system
Tem Morrison	Immunology and Microbiology	scRNA-seq	Mechanisms of chronic chikungunya virus infection and joint disease
Holger Russ	BDC; Immunology and Microbiology	scRNA-seq	Cellular heterogeneity and function in thymi of patients with Down syndrome
Charles Sagerstrom	Pediatrics-Developmental Biology	scRNA-seq	Adaptation of single-cell calling card technology to test TF function in zebrafish embryogenesis
Rebecca Schweppe	Medicine- Endocrinology, Metabolism, and Diabetes	scRNA-seq	Deregulation of the transcriptome promotes the transformation of anaplastic thyroid cancer
Lori Sussel	BDC	scRNA-seq	Identification of pancreatic islet cell-type specific splicing events
Benjamin Bitler	OBGYN	Spatial Transcriptomics	Spatial analysis of the ovarian cancer tumor microenvironment
Nicholas Foreman	Pediatrics-Hematology, Oncology, and Bone Marrow Transplantation	Spatial Transcriptomics	Spatial lineage trajectory analysis in childhood brain tumor medulloblastoma
Wendy Macklin	CDB	Spatial Transcriptomics	Spatiotemporal analysis of microglial contribution to immune-mediated demyelinating lesion formation
Rebecca McCullough	Pharmaceutical Sciences	Spatial Transcriptomics	The neuroimmune landscape of the aging brain: Dissecting the impact of alcohol
Julie Siegenthaler	Pediatrics- Developmental Biology	Spatial Transcriptomics	Spatial transcriptomics to study mechanisms of meninges development
Carmen Sucharov	Medicine- Cardiology	Spatial Transcriptomics	Spatial transcriptomics in the failing pediatric heart
Kelly Sullivan	Pediatrics- Developmental Biology	Spatial Transcriptomics	Defining the impact of interferon receptor copy number on neuroinflammation in a mouse model of Alzheimer’s disease in Down syndrome

RECIPIENT	DEPARTMENT	AWARD	PROJECT
Kristin Artinger	Craniofacial Biology	RNA-seq	Using RNA-seq to identify molecular interactions among different cell populations during neural crest cell migration
Adela Cota-Gomez	Pulmonary Sciences and Critical Care	RNA-seq	Nrf2 activator PB125 as a potential therapeutic agent against coronavirus (COVID related)
Tom Evans	CDB	RNA-seq	Discovering direct RNA targets of novel ncRNAs
Wendy Macklin	CDB	RNA-seq	Developmental RNAseq analysis of the translatome in axons with conditional neuronal Raptor deletion
Nidia Quillinan	Anesthesiology	RNA-seq	Distinct firing properties of thalamic neurons receiving cerebellar input
Olivia Rissland	BMG	RNA-seq	COVID genomic sequencing and tracking
Rebecca Schweppe	Pathology	RNA-seq	Nuclear FAK alters the transcriptome to promote thyroid cancer growth and survival
Linda van Dyk	Barbara Davis Center	RNA-seq	Identification of host and viral ncRNA interatcing with La and RIF-I during gammaherpesvirus infection
Natalia (Maria) Vergara	Opthamology	RNA-seq	Unraveling the molecular basis of retinal abnormalities in Down syndrome
Quentin Vicens	BMG	RNA-seq	Using RNA-seq to determine the biological role of the Z-RNA binding domain of ADAR1 during the interferon response
Rocky Baker	Immunology and Microbiology	scRNA-seq	Combined TCR and gene expression profile of HIP-reactive T cells in islet grafts after induction of tolerance with 2.5HIP PLG-NPs
David Beckham	Infectious Disease	scRNA-seq	Defining innate immune responses to SARS-CoV2 with single cell resolution
Melanie Koenigshoff	Pulmonary Sciences and Critical Care	scRNA-seq	Unraveling the molecular mechanisms of distal lung epithelial stem cell impairment in COPD
Liyen Loh	Immunology and Microbiology	scRNA-seq	Deciphering the development of innate T cells in the human thymus
James Nichols	Craniofacial Biology	scRNA-seq	Clonal dominance in appendage development
Matthew Sikora	Pathology	scRNA-seq	Functional heterogeneity in estrogen receptor co-regulator activity
Mary Weiser-Evans	Renal Diseases and Hypertension	scRNA-seq	SMC-derived AdvSca-SM cell contribution to cardiac fibrosis
Timothy McKinsey	Cardiology	scRNA-seq	Exploring the therapeutic potential of HDAC inhibition in HFpEF
Howard Davidson	Barbara Davis Center	scTech Development	Monitoring alternative splicing in single human T cells by ScISOr-Seq plus
Peter Dempsey	Gastroenterology, Hepatology, and Nutrition	scTech Development	Population-based analysis of intestinal organoid heterogeneity using CellTagging and scRNAseq
James Dylewski	Renal Diseases and Hypertension	scTech Development	The role of FcRn in antigen presentation by glomerular endothelial cells in immune mediated kidney disease
Rachel Friedman	Barbara Davis Center	scTech Development	Myeloid cells in the islets
Kirk Hansen	BMG	scTech Development	Development of an ECM atlas to guide regenerative medicine therapies using MULTI-seq
Elena Hsieh	Pediatric Allergy and Immunology	scTech Development	Understanding IL-15/IL-2RB interactions in a hypomorphic IL-2RB mouse model
Katja Kiseljak-Vassiliades	Endocrinology, Metabolism, and Diabetes	scTech Development	In situ reconstruction of distinct cell populations within the human adrenal gland
Christian Mosimann	Pediatrics	scTech Development	Charting cardiovascular progenitor clonality and developmental trajectories in toto
Eric Pietras	Hematology	scTech Development	Clonal tracking and expression analysis in a mouse model of human clonal hematopoiesis of indeterminate potential (CHIP)
Stephen Santoro	Peds-Developmental Biology	scTech Development	Lineage tracing the odorant receptor fates of olfactory sensory neurons
Eszter Vladar	Pulmonary Sciences	scTech Development	WNT4 signaling specifies ciliated cell fate in the airway epithelium
Tuoqi Wu	Immunology and Microbiology	scTech Development	Identify the transcriptional and protein expression signatures of CAR T cells with long-term antitumor immunity using endogenous TCR sequences as molecular barcodes

RECIPIENT	DEPARTMENT	AWARD	PROJECT
Howard Davidson	Barbara Davis Center	Bioinformatics Support	Using CITE-seq to define immune biomarkers of the honeymoon period in type 1 diabetes
Joshua Black	Pharmacology	RNA Biology	Enrichment detection and discovery of repeat element-gene mRNA fusion transcripts
Tom Evans	Cell and Developmental Biology	RNA Biology	Control of RNP co-assembly dynamics in early development
Tania Reis	Endocrinology, Metabolism, and Diabetes	RNA Biology	Mechanisms of control of Drosophila fat storage by conserved RNA-binding protein Nito
Douglas Shepherd & Diego Restrepo	Pharmacology	RNA Biology	High-throughput spatial quantification of RNA expression in mouse olfaction after exposure to viral agents
Jianbin Wang	BMG	RNA Biology	Transcription and transcription-replication collision and R-loop formationduring programmed DNA elimination
Diana Cittelly	Pathology	RNA-seq	Transcriptional profile of reactive astrocytes at early and late stages of brain metastatic progression
Katherine Fantauzzo	Craniofacial Biology	RNA-seq	Srsf3-mediated alternative RNA splicing downstream of PDGFRalpha signaling in the palatal mesenchyme
Jeffrey Kieft	BMG	RNA-seq	Identification of cellular exoribonuclease-resistant RNAs by xrRNA-seq
Traci Lyons	Oncology	RNA-seq	Investigating SEMA7A-regulated mechanisms of endocrine resistance in ER+ breast cancer
Michael McMurray	CDB	RNA-seq	Defining the maternal contribution of RNAs in yeast gametes
Chad Pearson	CDB	RNA-seq	Identifying centriolar RNAs in Tetrahymena thermophila
Rytis Prekeris	CDB	RNA-seq	Identification of midbody-associated RNA
Eszter Vladar	Pulmonary Sciences	RNA-seq	Molecular, physiological and health outcome impacts of triple combination therapy in cystic fibrosis airway disease
Trevor Williams	Craniofacial Biology	RNA-seq	Functional analysis of differential splicing by Rbm10 – the gene mutated in human TARP Syndrome
Rocky Baker	Immunology and Microbiology	scRNA-seq	Monitor phenotype and TCR of HIP-reactive T cells over time in a subject at risk for T1D
Joseph Brzezinski	Opthamology	scRNA-seq	Regulation of human cone photoreceptor development
Eric Clambey	Anesthesiology	scRNA-seq	CD4 T cell lineage tracing by scRNA-seq
Nicholas Foreman	Pediatrics	scRNA-seq	To investigate the feasibility of single-nuclei RNAseq analysis of pediatric brain tumors
Dany Gaillard	CDB	scRNA-seq	Single cell RNA-seq of irradiated mouse CVP
Ross Kedl	Immunology and Microbiology	scRNA-seq	In search of Tvacs: single cell RNAseq comparison between vaccine-elicited and infection elicited CD8+ T cell responses
T. Rajendra Kumar	OB/GYN	scRNA-seq	Single cell RNA-seq analysis of pituitary gonadotropes
Wendy Macklin	CDB	scRNA-seq	scRNAseq of oligodendrocytes during development with conditional rictor deletion in neurons
Karen Moulton	Cardiology	scRNA-seq	Translating vascular cell phenotypes of human coronary artery disease
Holger Russ	Barbara Davis Center	scRNA-seq	Elucidating AIRE dependent expression of tissue-restricted antigens in stem cell derived organotypic thymus cultures from healthy and autoimmune patients
Daniel Sherbenou	Hematology	scRNA-seq	Tracking resistant multiple myeloma cells in residual disease by single-cell RNA-seq
Tamara Terzian	Oncology	scRNA-seq	Molecular determinants of lymphangiogenesis

RECIPIENT	DEPARTMENT	AWARD	PROJECT
Lori Sussel	Barbara Davis Center	Bioinformatics Support	Identification of IncRNAs associated with obesity-induced diabetes
Kristin Artinger & David Bentley	Craniofacial Biology & BMG	RNA-seq	Developmental control of co-transcriptional splicing in zebrafish
Sue Kinnamon	Otolaryngology	RNA-seq	Transcriptome analysis of GAD65-expressing taste receptor cells
Suchitra Rao	Infectious Diseases	RNA-seq	Measures of immune responses to influenza infection in children
Lori Sussel	Barbara Davis Center	RNA-seq	Determine the effect of zinc deficiency on the islet transcriptome
Yael Aschner	Pulmonary Sciences and Critical Care Medicine	scRNA-seq	Analysis of ARDS BAL
Matthew Burchill	Gastroenterology and Hepatology	scRNA-seq	Dissection of the T lymphocyte activation landscape in NASH
David Clouthier	Craniofacial Biology	scRNA-seq	A transcriptional code coordinates jaw development
Howard Davidson	Barbara Davis Center	scRNA-seq	Profiling T cell responses to low affinity polyclonal stimuli
Jonathan Kurche & David Schwartz	Pulmonary and Critical Care	scRNA-seq	Single nuclei RNA sequencing in idiopathic pulmonary fibrosis (IPF)
Hong Li and Eriv Van Otterloo	Craniofacial Biology	scRNA-seq	Redefining the ameloblast lineage
Martin McCarter	Surgical Oncology	scRNA-seq	Analysis of tumor infiltrating MDSCs in human melanoma
Joanna Poczobutt & Oliver Eickelberg	Pulmonary and Critical Care	scRNA-seq	The role of MZB1-positive plasma B cells in idiopathic pulmonary fibrosis
Beth Tamburini	Immunology	scRNA-seq	Transcriptional profiling of lymphatic endothelial cells and dedritic cells during antigen archiving exchange
Ivana Yang	Pulmonary and Critical Care	scRNA-seq	Characterization of bronchoalveolar lavage cell populations from patients with sarcoidosis

RECIPIENT	DEPARTMENT	AWARD	PROJECT
Steve Anderson	Pathology	Bioinformatics Support	Analysis of the role of glucose metabolism upon tumor-initiating cells
David Bentley	BMG	Bioinformatics Support	The development of a bioinformatics pipeline to characterize nascent RNA structure
Nicholas Foreman	Hematology	Bioinformatics Support	Single-cell mRNA seq analysis of pedatric brain tumors
Bernice Gitomer	Renal Medicine	Bioinformatics Support	Identification of the etiology of the bone defect in patients with autosomal dominant polycystic kidney disease (ADPKD)
Eva Grayck	Cardiovascular and Pulmonary	Bioinformatics Support	Bioinformatic analysis of RNA sequencing data to interrogate the redox regulation of alveolar macrophage populations in acute lung injury
Craig Jordan	Hematology	Bioinformatics Support	Identification and characterization of drug-resistant subpopulations in AML
Elizabeth Wellberg	Pathology	Bioinformatics Support	Using gene expression profiles to predict obesity-associated breast cancer progression
Bruce Appel	Pediatrics, Stem Cell Biology	scRNA-seq	Single cell RNA-seq investigation of glial cell heterogeneity
Kristin Artinger	Craniofacial Biology	scRNA-seq	Single cell profiling of neural crest derived cancers
Linda Barlow	CDB	scRNA-seq	Single cell RNA-seq of Sox2-GFP lingual epithelial cells
Dick Davis	BMG	scRNA-seq	Single cell RNA-seq analysis of ascarsis development
Peter Dempsey	Gastroenterology, Hepatology, and Nutrition	scRNA-seq	Reversion of Defa4Cre-expressing Paneth cells to a stem cell state in response to intestinal injury
Robert Doebele	Oncology	scRNA-seq	Single cell analysis of oncogene-driven lung adenocarcinoma to determine mechanisms of residual disease following oncogene-targeted therapy
Patricia Ernst	Pharmacology	scRNA-seq	Enhancing hematopoietic stem cell emergence from pluripotent embryonic stem cells using MLL1 induction
James Nichols	Craniofacial Biology	scRNA-seq	Understanding jaw evolution by comparing craniofacial progenitor cells in lamprey and zebrafish
Holger Russ	Barbara Davis Center	scRNA-seq	Elucidating human beta cell subtype regulation and interconversion in health and disease
Howard Davidson	Barbara Davis Center	scRNA-seq	Single cell profiling in type 1 diabetes
Joan Hooper	CDB	scRNA-seq	Analysis of craniofacial development
Lori Sussel	Barbara Davis Center	scRNA-seq	Analysis of pancreatic progenitors

RECIPIENT	DEPARTMENT	AWARD	PROJECT
Erin Baschal	Orthopedics	Clinical	Comparison of facet joint cartilage RNA from adolescent idiopathic scoliosis patients and control individuals
Kathleen Connell	Urogynecology and Reconstructive Surgery	Clinical	Epigenetic and RNA expression profiles in women with pelvic organ prolapse
James Costello	Pharmacology	Clinical	RNA sequencing analysis of bladder cancer cell lines that localize to the lung and liver
Tom Evans	CDB	Seed Grant	Identification of novel RNA targets of sm-rings
Bernice Gitomer	Renal Diseases and Hypertension	Seed Grant	Prediction of the rate of autosomal dominant polycystic kidney disease progression based on circulating miRNA levels
Aaron Johnson	BMG	Seed Grant	m6A RNA modification in long noncoding RNA matchmaking and heterochromatin formation
Sandy Martin	CDB	Seed Grant	Investigating RNA editing in the brain of mammalian hibernator and Investigating transcript isoform dynamics in a hibernator
Lee Niswander	Molecular, Cellular & Developmental Biology	Seed Grant	An alternative splicing mechanism in the lincRNA-1354 locus commits embryonic stem cells to neural differentiation
Eric Pietras	Hematology	Seed Grant	Role of RNA splicing factor U2AF1 mutations in regulating hematopoietic stem cell inflammation response and myelodysplastic syndrome
Jennifer Richer	Pathology	Seed Grant	Targeting the long non-coding RNA MALAT1 in ovarian cancer
Mario Santiago	Infectious Diseases	Seed Grant	Noncoding RNAs in mucosal HIV-1 persistence and pathogenesis
Carol Sartorius & Peter Kabos	Pathology & Oncology	Seed Grant	Single cell analysis of organ specific metastasizing cells in estrogen receptor positive breast cancer
Lori Sussel	Barbara Davis Center	Seed Grant	lncRNA discovery in motor neurons
Trevor Williams	Craniofacial Biology	Seed Grant	Probing the consequences of the disruption of epigenetic bookmarking in human Branchio-oculo-facial Syndrome using single-cell sequencing

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