Rates and Fee Structures
Pre-clinical Human Immune System Mice Share Resource (PHISM) Services and Fee Structure for users within the CU system. Prices below are subsidized by the Human Immunology and Immunotherapy Dean’s Transformation Initiative (CU School of Medicine).
Further reduced rates are available for preliminary data that will be used in future grant applications, please inquire if your study qualifies.
Users outside the CU system, please inquire about non-academic, non-subsidized
pricing.
EXPERIMENTAL DESIGN SUPPORT
| Service | Description | Pricing |
| Initial Project Support | Discussion of research project needs, a high-level view of experimental design and timeline, and associated analyses and services | Free |
| Experimental Design Support | Design of experiment including number of mice, treatments, time-course and flow cytometry panel design for analysis of Human Immune System mouse tissue | $406 |
MICE
| Service | Description | Pricing |
| BRGS/NBSGW | BRGS (BALB/c Rag2-/- IL2Rγ-/- SIRPαNOD mice) or NBSGW (NOD.CgKitW41J Tyr+ Prkdcscid Il2rgtm1Wjl/ThomJ) | $17 + cage cost |
| HIS-CB-BRGS | Human Immune System mice generated from CD34+ HSC’s from cord blood at birth verified by two bleeds to be >25% human, >14 weeks | $364/mouse |
| • With full flow immune analysis (tissue harvest and Stains A-D, see below) | $1,394/mouse | |
| • With customized flow immune analysis | Contact for pricing | |
| HIS-PBMC BRGS | Human Immune System mice generated from peripheral blood mononuclear cells (PBMC) from adult blood or cord blood and injected into adult BRGS immunodeficient mice. Human chimerism verified at 5-14 days post injection. Mice develop GVHD and should be used between 2-4 weeks and typically reconstitute with a predominant activated T cell population and approximately 10% B cells. Model is validated for Cytokine Release Syndrome assays testing inflammatory markers in blood following treatments. | $261/mouse |
| HIS-FLT-BRGS | Human Immune System mice using CD34+ fetal liver (FL) hematopoietic stem cells (HSC) and co-implantation of matched thymus. Human Immune System mice are verified for >25% human chimerism in blood. | Contact for pricing |
| HIS-BM-BRGS | Human Immune System mice using CD34+ from bone marrow (BM) hematopoietic stem cells (HSC) at birth and verified for >5% human chimerism in blood | Contact for pricing |
CUSTOM FLOW ANALYSIS
| Service | Pricing |
| Flow Time | $150/hour |
| Antibody cost | $4/sample or list price |
| Panel design and staining | $133/hour + antibodies + flow time |
Stain A (EC only) (human immune subsets) | $261/mouse |
Stain B (EC only) (HLA, inhibitor receptor expression on tumor and immune cells) | $173/mouse |
Stain C (IC) Tregs, Tem/cm, Granzyme B | $202/mouse |
Stain D (IC) Cytokies (IFNγ, TNFα) | $196/mouse |
HARVEST/Misc
| Service | Pricing |
| HIS Mouse lymph node and spleen harvest | $126/mouse |
| HIS Mouse Tissue (eg tumor, lung, liver) harvest | $152/mouse |
| Tumor injections | $12/mouse |
| Tumor measurement | $12/mouse |
| Drug Dosing | $6/mouse |
| CD34+ isolation from cord blood | $1,029 per CB |
Contact
Julie Lang, PhD
Assistant Director, Human Immune System Mouse Core
303-724-8705
Julie.Lang@cuanschutz.edu