Services Price List


Primary Cell Lines Isolation/Reprogramming

The core uses RNA-based and episomal-based reprogramming technology to reprogram somatic cells into induced pluripotent stem cells (iPSCs).

ServiceDescriptionPrice
Fibroblast IsolationIncludes isolation, expansion, and freezing of fibroblasts from skin biopsy (mycoplasma tested)$692.11
Fibroblast reprogrammingRNA‐based reprogramming
Deliver 2‐4 clonally expanded iPSC lines (2‐4 frozen vials per iPSC line, identity and mycoplasma tested)
$2,927.18
Renal epithelial cell (REC) isolationIncludes isolation, expansion, and freezing of RECs from urine sample (mycoplasma tested)$755.81
REC ReprogrammingRNA‐based reprogramming
Deliver 2‐4 clonally expanded iPSC lines (2‐4 frozen vials per iPSC line, identity and mycoplasma tested)
$3,155.41
Peripheral blood mononuclear cell (PBMC) isolationIncludes isolation and freezing of PBMCs (1‐2 vials)$369.73
PBMC ReprogrammingEpisomal reprogramming
Deliver 2‐4 clonally expanded iPSC lines (2‐4 frozen vials per iPSC line, identity and mycoplasma tested, loss of episomes confirmed by PCR)
$2,859.58

iPSC Characterization Services and Quality Control

The core provides services to analyze newly reprogrammed (or client provided) iPSC lines to provide a complete characterization profile and demonstrate pluripotency.

ServiceDescriptionPrice
Thaw iPSCsThaw vial into two wells of a 6‐well plate$151.84
Expand and Freeze iPSCsFreeze 8‐12 vials$331.28
Mycoplasma TestingAll samples are tested for mycoplasma contamination$83.18
Identity testingSTR analysis$110.00
Pluripotency AnalysisImmunofluorescence for TRA‐1‐60, NANOG, OCT 3/4$287.02
 qPCR for OCT4, NANOG, DNMT3B$283.77
G‐Band KaryotypingChromosome analysis, optimized for human iPSCs$679.83

Gene Editing

  • Investigator can provide iPSCs or use a SCBDMC control iPSC line (+$434)
  • Includes design of gene modification strategy and purchase of all reagents
  • iPSCs are modified with Cas9 mRNA and synthesized gRNA
ServiceDescriptionPrice
Gene knock‐out by INDELModified iPSCs are clonally expanded and the INDEL mutation is confirmed by Sanger sequencing
Deliver 4‐6 frozen vials (identity and mycoplasma tested)
$9,848
SNP correction or introductionModified iPSCs are clonally expanded and the desired mutation is confirmed by Sanger sequencing
Deliver 4‐6 frozen vials (identity and mycoplasma tested)
$13,031
Targeted fluorescence knock‐inUp to 24 genetically modified iPSC lines are clonally expanded 
Deliver 2 frozen vials (identity tested)
 IMPORTANT: We cannot functionally validate successful targeting for genes that require differentiation. Reporter validation and clonality must be confirmed by the investigator.
$11,442
Constitutive fluorescence knock‐inmTagBFP2, mWasabi, tdTomato, iRFP670
Most FPs can be localized to the nucleus (NLS) or membrane (CAAX)
Clonally expanded iPSC line (3‐4 frozen vials, identity and mycoplasma tested)
$3,573

Modified mRNA

Subject to availability

ServiceDescriptionPrice
Modified mRNAIn stock modified mRNAs (per µg); bulk discounts up to 30%$12.16
RNA Reprogramming KitSufficient mRNA/miR cocktail and transfection buffer to reprogram a full 6‐well plate$677.71
Custom modified mRNACloning cDNA template and mRNA synthesisInquire

Biorepository

The core is equipped with a top of the line setup for cell cryopreservation and storage. Our Isothermal Liquid Nitrogen Freezers are safe; provide no cross-contamination and the best in the industry for temperature uniformity.

ServiceDescriptionPrice
Vial StoragePrice per vial per month$0.06
Vial of wild‐type iPSCsDerived from fibroblasts, reprogrammed with RNA$434.41
Vial of modified iPSCsConstitutively fluorescent iPSCs (mWasabi; strong CAGG promoter, targeted to AAVS safe)
Core generated genetically modified iPSCs
$720.13

Other Services

ServiceDescriptionPrice
iPSC TrainingHands‐on training includes thawing, culture, passaging, and freezing of human iPSCs$1,197.53
ConsultingExample expertise includes but is not limited to: CRISPR design, custom assay design (eg ddPCR), viral vector design, custom mRNA production, training, etc)$111.51/h

Listed prices are for the researchers affiliated with the University of Colorado. External clients need to contact the core to inquire about external prices.

 

Click here for PDF of our full price list.