Modified mRNA

n vitro-transcribed mRNA offers several distinct advantages for ectopic gene expression compared to more common plasmid- or viral-based expression systems:
  • Non-integrating - Eliminates the possibility of insertional mutagenesis
  • Efficient Delivery – mRNA only needs to cross a single plasma membrane to enter the cytoplasm, where translation occurs. mRNA can have significantly higher transfection efficiencies than plasmids in some cell lines
  • Controlled expression – Dosage can be finely tuned by simply transfecting more or less mRNA
  • Rapid expression – Functional protein is translated quicker since transcription/polyadenylation/nuclear export does not need to occur
  • Transient expression – Plasmid- and viral-based expression can be detrimentally stable, or even permanent if random DNA integration occurs

 

Features of our modified mRNA:

  • 100% modified using 5-methyl-cytosine (5mC) and pseudouridine (ψ) to better mimic endogenous mRNA and reduce innate-immunity stimulation
  • Poly(A) tail introduced via transcription template so all Poly(A) tails are identical in length
  • Capped using anti-reverse cap analog (ARCA) for increased mRNA translation efficiency
  • DNase treated to remove DNA transcription template
  • Phosphatase treated to further reduce innate immune response
  • 5’ UTR includes Kozak consensus sequence for optimal translation efficiency
  • 3’ UTR derived from human β-globin gene, shown to increase mRNA stability
  • Highly-competitive price
  • Bulk discounts are available (up to 30% off)

The SCB&DM core has a growing library of mRNAs for purchase. We can also provide custom cloning and synthesis services for your gene of interest. Contact us​​ to inquire about custom orders to suit your experimental needs.​

 
Reprogramming mRNAs
​mRNA​Notes
Pre-Mixed Reprogramming mRNAs 3:1:1:1:1:1 mix of
M3O​: SOX2 : KLF4 : cMYC : LIN28A : NANOG
M3O​​​​​Human OCT4 fused to MyoD transactivation domain
SOX2Human
KLF4Human
cMYC​Human​
LIN28AHuman
NANOG​Human
OCT4Human
 
Cas9 variants
​mRNA​Notes
​​SpCas9wild-type Cas9​​
eSpCas9(1.1)High Fidelity Cas9
HypaCas9High Fidelity Cas9
SpCas9nCas9 nickase
SpCas9-hGEMCas9 fused to Geminin for enhanced HDR
dCas9-VPRDead SpCas9 fused to transcriptional activator

Other useful cDNAs
​mRNA​Notes​
PuroR​​Puromycin Resistance​​
CreCre Recombinase
BlastR​Blasticidin Resistance
 
Fluorescent proteins
​​ ​mRNA​Excitati​on ​​​​(nm)​Emmision ​(nm)​Estimated ​Brightness​ ​Tags​​ ​Notes
mTagBFP2P2​​
​399​454​32.4
​mTagBFP2​399​454​32.4​3xFlag, nls​localized to nucleus
​T-Sapphire​399​​511​26.4​Long stokes shift
​mCerulean3​433​475​32
​d2EGFP​488​507​33.6​Destabilized EGFP
​mWasabi​493​509​56
​mWasabi​​493​50956​3xFlag, nls​​localized to nucleus
​Clover​505​515​84
​Clover-P2A- ​PuroR​505 ​​515 ​​ ​​ ​​Bicistronic expression of Clover and Puromycin resistance ​
​nls-Clover​505​515​84​3xFlag, nls​localized to nucleus
​YPET​517​530​80
​tdTomato​554​581​95.2​3xFlag, nlslocalized to nucleus
​tdTomato-P2A- ​Blast​554 ​​581 ​​ ​​Bicistronic expression of tdTomato and Blasticidin resistance
​nls-tdTomato​554​581​95.2​3xFlag, nls​localized to nucleus
​mRuby2​559​600​43
​mRuby2-P2A ​Blast​559 ​​600 ​​ ​​ ​​Bicistronic expression of mRuby2 and Blasticidin resistance
​iRFP670​643​670​12.5
​​iRFP670​643​670​12.5​​3xFlag, CAAX​​localized to membrane​​

*Brightness = extinction coefficient x quantum yield.
For reference, EGFP brightness = ~34.