Gene Editing

The core utilizes the CRISPR/Cas9 system to achieve gene editing in iPSCs and somatic cells.


​​Experimental Design, Vector Assembly, Validation
​​Targeting strategy and design​1 week
​Vector Construction​1-4 weeks
​gRNA synthesis​1 week
​Transfection into target cells​1-2 weeks
​Genome editing verification​1-2 weeks
Transfection into cells, INDEL confirmation
​Transfection into target cells​1-2 weeks​
​Genome editing verification​​1-2 weeks
Single-cell expansion, genome editing confirmation
​Picking/expanding clones​2-4 weeks
​Colony screening (T7E1, ddPCR, etc.)​2-3 weeks
​Sanger Sequencing​2-3 weeks
iPSC clone characterization, expansion, biobanking
​Cell recovery, culturing, expansion​2-4 weeks
​Sequencing Targeted Region​1-2 weeks
​Pluripotency Staining​1 week
​Karyotyping​2 weeks
​Mycoplasma​1 week
​Freezing​1 week
Total​(20-35 weeks)​