The quantitative PCR core facility in the division of Endocrinology at UCDenver has been in operation since 1998 as a service facility to more than 100 investigators on and off campus. The core facility uses a QuantStudio 7 Flex Real time PCR instrument. Acknowledged as the gold standard in q-PCR, the QuantStudio 7 Flex system combined with the TaqMan genomic assays provides high throughput and flexibility, allowing users to pursue projects beyond the scope of any other Real time instrument. In collaboration with the Animal facility on campus, highly experienced core personnel have designed and optimized assays to detect sensitive levels of Corynebacterium bovis. The dedicated core lab space utilizes sterile equipment and aseptic techniques to provide quality data with a level of certainty that is useful for diagnostic research. Furthermore, an efficient turnaround time of 3-4 business days avoids potential delays in data progression.
I. Quantitated riboprobe (absolute standard) or invitro transcribed RNA
II. RNA from source with known expression of gene (relative standard)
III. Plasmid-DNA (absolute PCR standard). If you are not sure about a positive control, then we can place the order for the amplicon as a single stranded DNA and use it for std curve.
|RNA Extraction - 5 samples||$223|
|RNA Extraction - 10 samples||$334|
|RNA Extraction - 15 samples||$416|
|RNA Extraction - 20 samples||$553|
PCR Assay - C. Bovis
PCR Assay - MPV & MVM